Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

Abstract

Erythrodiol is a terpenic compound found in a large number of plants. To test the hypotheses that its long-term administration may influence hepatic transcriptome and this could be influenced by the presence of APOA1-containing high-density lipoproteins (HDL), Western diets containing 0.01% of erythrodiol (10 mg/kg dose) were provided to Apoe- and Apoa1-deficient mice. Hepatic RNA-sequencing was carried out in male Apoe-deficient mice fed purified Western diets differing in the erythrodiol content. The administration of this compound significantly up- regulated 68 and down-regulated 124 genes at the level of 2-fold change. These genes belonged to detoxification processes, protein metabolism and nucleic acid related metabolites. Gene expression changes of 21 selected transcripts were verified by RT-qPCR. Ccl19-ps2, Cyp2b10, Rbm14-rbm4, Sec61g, Tmem81, Prtn3, Amy2a5, Cyp2b9 and Mup1 showed significant changes by erythrodiol administration. When Cyp2b10, Dmbt1, Cyp2b13, Prtn3 and Cyp2b9 were analyzed in female Apoe-deficient mice, no change was observed. Likewise, no significant variation was observed in Apoa1- or in Apoe-deficient mice receiving doses ranging from 0.5 to 5 mg/kg erythrodiol. Our results give evidence that erythrodiol exerts a hepatic transcriptional role, but this is selective in terms of sex and requires a threshold dose. Furthermore, it requires an APOA1-containing HDL.

Hepatic galectin-3 is associated with lipid droplet area in non-alcoholic steatohepatitis in a new swine model

“…Supplementary Table S6 lists the most strikingly influenced genes by the steatotic diet (log 2 fold change < − 2.5 and > 4.5). Based on our previous experience, where only transcripts whose levels were higher than 0.3 fragments per kilo base per million mapped reads (FPKM) and readings displayed in more than 75% of samples, were reliably confirmed by RT-qPCR 39 , a meticulous screening of readings was undertaken. As shown in Fig.…”

Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins

“…The resulting 6 samples were sent to the Beijing Genomics Institute (BGI Genomics, Shenzhen, China) service. RNA quality tested, library construction, sequencing reads and posterior clean, genome mapping, analysis, identification, and quantification were realized as previously described [36]. A Bioinformatics flow of about 4.52 Gb per sample had an average genome mapping rate of 93.1%.…”

“…To verify the most relevant changes induced by the absence of TXNDC5 using RNAseq, represented by signal log 2 ratio > 1.0 or <−1.0 and a p value < 0.005 for upregulated and downregulated, respectively, 10 genes fulfilling these criteria were chosen. Primer design (Supplementary Table S1), RT-PCR and cDNA synthesis were carried out as previously described [36]. ViiA7 Real-TIME PCR System (Life Technologies) was used, and relative amount of mRNA was calculated using the comparative 2 −∆∆Cq method and normalized to the reference Ppib expressions.…”

Ursolic Acid and Related Analogues: Triterpenoids with Broad Health Benefits

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