Dietary Curdlan Increases Proliferation of Bifidobacteria in The Cecum of Rats

Shimizu, Jun; Tsuchihashi, Noboru; Kudoh, Keita; Wada, Masahiro; Takita, Toshichika; Innami, Satoshi

doi:10.1271/bbb.65.466

Cited by 29 publications

(19 citation statements)

References 13 publications

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“…In vitro and animal trials support that high barley ␤-glucan consumption is associated with a significant increase in the synthesis of SCFA (46,49,50). This study confirmed the previous GC-MS data showing a noticeable increase in 2-methyl-propanoic acid, acetic acid, butanoic (butyric) acid, and propanoic (propionic) acid after the diet intervention with durum wheat flour and whole-grain barley pasta.…”

Section: Discussionsupporting

confidence: 88%

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Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome

Angelis

Montemurno

Vannini

et al. 2015

Appl Environ Microbiol

131

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In this study, we compared the fecal microbiota and metabolomes of 26 healthy subjects before (HS) and after (HSB) 2 months of diet intervention based on the administration of durum wheat flour and whole-grain barley pasta containing the minimum recommended daily intake (3 g) of barley ␤-glucans. Metabolically active bacteria were analyzed through pyrosequencing of the 16S rRNA gene and community-level catabolic profiles. Pyrosequencing data showed that levels of Clostridiaceae (Clostridium orbiscindens and Clostridium sp.), Roseburia hominis, and Ruminococcus sp. increased, while levels of other Firmicutes and Fusobacteria decreased, from the HSB samples to the HS fecal samples. Community-level catabolic profiles were lower in HSB samples. Compared to the results for HS samples, cultivable lactobacilli increased in HSB fecal samples, while the numbers of Enterobacteriaceae, total coliforms, and Bacteroides, Porphyromonas, Prevotella, Pseudomonas, Alcaligenes, and Aeromonas bacteria decreased. Metabolome analyses were performed using an amino acid analyzer and gas chromatography-mass spectrometry solidphase microextraction. A marked increase in short-chain fatty acids (SCFA), such as 2-methyl-propanoic, acetic, butyric, and propionic acids, was found in HSB samples with respect to the HS fecal samples. Durum wheat flour and whole-grain barley pasta containing 3% barley ␤-glucans appeared to be effective in modulating the composition and metabolic pathways of the intestinal microbiota, leading to an increased level of SCFA in the HSB samples.

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Section: Discussionsupporting

confidence: 88%

“…On the contrary, oat ␤-glucans did not favor the growth of Clostridium sp. in SHIME and C57BL/6J mouse models (21,24,49). However, several discrepancies between the different studies might be due to the different models and methods used.…”

Section: Discussionmentioning

confidence: 99%

Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome

Angelis

Montemurno

Vannini

et al. 2015

Appl Environ Microbiol

131

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“…Fermentation of b-glucan fractions from barley and oats by human faecal microbiota induced high clostridial cluster IX populations and a significant increase in Clostridium histolyticum subgroup (Hughes et al, 2008). On the contrary, oat bran feeding in SHIME model and curdlan diets in rat experiments did not favor the growth of Clostridium spp., respectively Shimizu et al, 2001).…”

Section: Tablementioning

confidence: 99%

Prebiotic potential of barley derived β-glucan at low intake levels: A randomised, double-blinded, placebo-controlled clinical study

Mitsou

Panopoulou

Turunen

et al. 2010

Food Research International

116

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“…Bifidobacteria have been cultured from the cecal contents of chickens (31,35,51), rats (43), rabbits (20,35,46), pigs (37), and humans (21). Isolates were confirmed as belonging to the genus Bifidobacterium in all but the rat and pig studies, through the detection of fructose-6-phosphate phosphoketolase (F-6-PPK) (41).…”

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confidence: 99%

Genomic Diversity and Relatedness of Bifidobacteria Isolated from a Porcine Cecum

et al. 2003

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This study initially involved the isolation of a number of bifidobacteria from either the lumen or the epithelium of a porcine cecum. A total of 160 isolates were selected at random on MRS plates containing cysteine hydrochloride (0.5 g/liter) and mupirocin (50 mg/liter). All were identified as bifidobacteria based on fructose-6-phosphate phosphoketolase activity. Following genomic digestion with the restriction enzyme XbaI and pulsed-field gel electrophoresis (PFGE), the isolates produced 15 distinct macro-restriction patterns. Several of the PFGE patterns differed by only 1, 2, or 3 DNA fragments and were grouped as related patterns into seven PFGE types, termed A through G. The related patterns appeared to show genomic plasticity within the isolates arising from chromosomal mutations or possibly horizontal transfer of plasmids. The relative frequency of each PFGE type was maintained within each cecal sample, with PFGE type E representing approximately 50% of the isolates. Randomly amplified polymorphic DNA PCR, cell morphology, whole-cell protein profiling, 16S ribosomal DNA sequencing, and DNA-DNA hybridization were used to determine if the seven apparently unrelated PFGE types represented genetically distinct isolates. Four groups were identified: PFGE types A, C/D/G, B/E, and F, and these appeared to represent Bifidobacterium minimum, Bifidobacterium pseudolongum subsp. pseudolongum, and Bifidobacterium pseudolongum subsp. globosum and two new species, respectively. The data demonstrate the presence of considerable genomic diversity within a relatively simple bifidobacteria population, consisting of 15 distinct strains representing four groups, which was maintained throughout the porcine cecal contents and epithelial layer.

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Dietary Curdlan Increases Proliferation of Bifidobacteria in The Cecum of Rats

Cited by 29 publications

References 13 publications

Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome

Effect of Whole-Grain Barley on the Human Fecal Microbiota and Metabolome

Prebiotic potential of barley derived β-glucan at low intake levels: A randomised, double-blinded, placebo-controlled clinical study

Genomic Diversity and Relatedness of Bifidobacteria Isolated from a Porcine Cecum

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