Dietary chromium effects on tissue chromium concentrations and chromium absorption in rats

Abstract

Chromium (Cr) absorption is low (<1%) and there is a need to find Cr compounds that are absorbed better than inorganic Cr salts. Therefore, the incorporation of nine different chromium (Cr) compounds on tissue Cr concentration of 6‐week male Wistar rats was investigated. Chromium compounds tested were Cr chloride (Cr chloride), Cr acetate (Cr acetate), Cr potassium sulfate (CrAlum), Cr trihistidine (Cr histidine), Cr triglycine (Cr glycine), Cr trinicotinic acid (CrNA), Cr dinicotinic acid dihistidine (CrNA‐HIS), Cr tripicolinic acid (Cr picolinate), and Cr dinicotinic acid diglycine cysteine glutamic acid (CrNA‐AA). Complexes were fed to weanling rats for 3 weeks at 5,000 ng of Cr/g of diet. Basal control diet was a cornstarch‐based diet containing 30 ng Cr/g. Chromium incorporation into the kidney was greatest for CrNA‐AA complex (850 ng/g dry wt) followed by CrAlum (407 ng/g), Cr acetate (397), CrNA‐HIS (394), Cr picolinate (368), Cr glycine (343), Cr nicotinate (166), Cr chloride (74), CrHIS (49), and control (23 ng/g). Chromium concentration of the liver was greatest for the Cr picolinate compound (50 ng/g) followed by CrNA‐AA and Cr acetate. Liver Cr concentrations of remaining complexes were not significantly different from those of the control animals that received no added Cr. Chromium concentrations were significantly greater in the kidney than those for the liver, spleen, heart, lungs, and gastrocnemius muscle. Supplemental Cr did not affect tissue zinc and copper but did alter tissue iron concentrations. Absorption of radioactive forms of Cr did not explain the differences in tissue Cr concentrations. Chromium absorption after 4 hours and retention after 24 hours were not significantly different for the forms of Cr tested. These data demonstrate that Cr concentrations are greatest in the kidney and that the form of dietary Cr significantly affects tissue Cr concentrations. Absorption of Cr does not correlate with tissue Cr concentrations and blood Cr is not in equilibrium with tissue Cr stores. © 1996 Wiley‐Liss, Inc. This article is a U.S. Government work and, as such, is in the public domain in the United States of America.

Effects of chromium nanoparticle dosage on growth, body composition, serum hormones and tissue chromium in Sprague-Dawley rats

“…In this study, Cr contents in liver and kidney were significantly increased with the addition of Cr as CrNano in the dosage of from 150 ppb to 1200 ppb. This is consistent with Anderson et al(1996), who reported that there was an approximate doubling of kidney Cr concentration and roughly 50% increased liver Cr concentration with the supplementation of 300 ppb Cr as Cr picoliante in the diets of pigs. Their results from rats (Anderson et al, 1996) showed that increase in tissue Cr following Cr supplementation were greatest for the kidney, followed by the liver, with considerably smaller changes in the heart and skeletal muscle tissues.…”

“…Previous studies indicated that supplemental Cr had effects on Cr content in tissues, but the results varied (Chang and Mowat, 1992;Anderson et al, 1996). In this study, Cr contents in liver and kidney were significantly increased with the addition of Cr as CrNano in the dosage of from 150 ppb to 1200 ppb.…”

“…Tissue Cr content was determined by the method of Anderson et al(1996) with atomic absorption spectrometry (AA6510, SHIMADZU, Japan).…”

Trivalent chromium (CrIII) as a trace element essential for animals and humans

“…Dowiedziono m.in. braku konsekwencji zdrowotnych związanych z bioakumulacją dużych dawek pikolinianu chromu (Cr +3 ) u szczurów (6). Nie zauważono również efektów genotoksycznych po propionianie Cr (III) (94).…”

“…Wykazano w badaniach na szczurach, że absorpcja Cr w formie nikotynianu i pikolinianu jest wyższa niż w formie chlorku (6). Podobną prawidłowość wykazano u ludzi, u których absorpcja Cr w formie chlorku była niższa względem pikolinianu i Cr podawanego w postaci drożdży.…”