Dietary butyrate suppresses inflammation through modulating gut microbiota in high-fat diet-fed mice

Abstract

Butyrate, a key metabolite fermented by gut microbiota mainly from undigested carbohydrates such as dietary fibers is widely used as feed additive. However, mechanisms of its contributions in maintaining host health are relatively poorly revealed. The aim of this study was to investigate how butyrate impacts gut microbiota and immunity response in high-fat diet-fed mice. Gut microbial analysis exhibited that butyrate intervention increased SCFAs-producing bacteria and decreased pathogenic bacteria, such as endotoxin-secreting bacteria. Our result also demonstrated that butyrate intervention enhanced fecal SCFAs concentrations, and inhibited endotoxin levels in feces and serum. Correlation analysis indicated positive relation between endotoxin level and Desulfovibrionaceae abundance. Furthermore, butyrate intervention inhibited expressions of IL-1β, IL-6 and MCP1/CCL2 in liver, as well as TLR4 in adipose tissue. Apart from inhibiting expressions of proinflammatory cytokines, butyrate exerted anti-inflammation effect through selectively modulating gut microbiota, such as increasing SCFAs-producing bacteria and decreasing endotoxin-secreting bacteria, as well as via regulating levels of microbiota-dependent metabolites and components, such as SCFAs and endotoxin.

Primary human colonic mucosal barrier crosstalk with super oxygen-sensitiveFaecalibacterium prausnitziiin continuous culture

“…117 This inhibitory effect of butyrate on TLR4 expression was also observed in mouse adipose tissue in vivo. 118 Sodium butyrate significantly decreased the TLR4 and NFKB signaling pathways in lipopolysaccharide-induced acute lung injury in mice. 119 In addition, butyrate inhibits the NF-kB pathway in the HT-29 colon cancer cell line.…”

Metabolic Responses to Butyrate Supplementation in LF- and HF-Fed Mice Are Cohort-Dependent and Associated with Changes in Composition and Function of the Gut Microbiota

“…Increasing attention has been paid to butyrate for its protective role against the pathophysiological processes of obesity [11]. Butyrate has been shown to have a number of different effects on the host including improvement of gut barrier function [12], suppression of the pro-inflammatory response [13,14], and secretion of gut hormones, such as glucagon-like peptide-1 [15], all of which could contribute to alleviation of obesity and related metabolic abnormalities. Butyrate has also been shown to decrease food intake and increase cholecystokinin (CCK)-induced satiety and reduce hepatic fat accumulation; there is evidence to suggest that these actions are mediated via an effect on the gut-brain neural circuit [16].…”

Nutrient-imbalanced Conditions Shift the Interplay Between Zooplankton and Gut Microbiota

“…This might be due to the effects of decreased microbial synthesis of butyrate in the gut (Fig. 7C), since it has previously been reported that Planctomycetaceae are more abundant in guts containing lower levels of butyrate (Zhai et al 2019). Both the P-and N-limited groups had more Streptococcaceae in their gut; indeed, previous reports indicate that the presence of Streptococcaceae in the gut is highly associated with metabolic disorders of the host (Aran et al 2011, Qiao et al 2014.…”