Dietary boron supplementation enhances sperm quality and immunity through influencing the associated biochemical parameters and modulating the genes expression at testicular tissue

Krishnan, Binsila B.; Selvaraju, Sellappan; Gowda, N. K. S.; Subramanya, Karthik Bhat; Pal, D. K.; Archana, Santhanahalli Siddalingappa; Bhatta, Raghavendra

doi:10.1016/j.jtemb.2019.05.004

Cited by 14 publications

(8 citation statements)

References 54 publications

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“…26,27 Dietary boron supplementation at 40 ppm increased sperm motility, sperm output as well as immune and antioxidant defense capacity in male goats. 28 It has been shown before that 100, 200, and 400 mg boric acid supplement can improve semen quality and has a positive effect on physiological condition in rabbits. 29 In spite of its advantages in this field, the toxic effect of high doses of boron on reproduction has been reported.…”

Section: Introductionmentioning

confidence: 99%

“…The mean safe daily intake of boron for adults was limited and endorsed (1–13 mg/day) by the World Health Organization (WHO) which intimates that intakes <1.0 mg/day is insufficient for optimal boron beneficial activity in both animal and human 26,27 . Dietary boron supplementation at 40 ppm increased sperm motility, sperm output as well as immune and antioxidant defense capacity in male goats 28 . It has been shown before that 100, 200, and 400 mg boric acid supplement can improve semen quality and has a positive effect on physiological condition in rabbits 29 .…”

Section: Introductionmentioning

confidence: 99%

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Combination of trehalose and low boron in presence of decreased glycerol improves post‐thawed ram sperm parameters: A model study in boron research

et al. 2021

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Background: Sperm cryopreservation has been widely used in the field of reproductive biotechnology. It applies to certain males of economic and scientific values, including livestock breeds or endangered animal species. The development of a semen extender with a low cryoprotectant concentration and an appropriate amount of trehalose and boron can prevent the deterioration of sperm parameters. Objective:The main goal of this study is to establish a suitable ram extender model, by examining different combinations of high (5%) and low (3%) glycerol concentrations (to reduce its toxic effects on sperm freezing), a fixed amount of trehalose and an increased dose of boron to prevent the deterioration of sperm parameters, and investigate the levels of gene expressions. Materials and methods:The Merino ram ejaculates were collected. The collected ejaculates providing the defined criteria were pooled. The pooled ejaculates were divided into eight aliquots and diluted with the Tris extender including different combinations of glycerol (5% and 3%) and boron (0.25, 0.5, and 1 mM) concentrations and a fixed amount of trehalose, then frozen. After freeze-thawing process, sperm motility, mitochondrial membrane activity, plasma membrane integrity, acrosomal membrane integrity, DNA damage (single cell gel electrophoresis (COMET) and TUNEL assays)

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combination of trehalose and low boron in presence of decreased glycerol improves post‐thawed ram sperm parameters: A model study in boron research

et al. 2021

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“…For instance, boric acid, one of the major boron compounds, has been categorized as a “Category 1B” compound in terms of developmental toxicity and fertility by the European Union (Bolt et al, 2020). Also, addition of boron to the diet of male goats was reported to cause some improvement in sperm movement and yield, as well as in immune and antioxidant systems (Krishnan et al, 2019). However, certain boron compounds may cause genotoxic effects in rats (Marat et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…. Also, addition of boron to the diet of male goats was reported to cause some improvement in sperm movement and yield, as well as in immune and antioxidant systems (Krishnan et al, 2019).…”

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confidence: 99%

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Demir

2022

J of Applied Toxicology

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Boron trioxide nanoparticles (B 2 O 3 NPs) have recently been widely used in a range of applications including electronic device technologies, acousto-optic apparatus fields, and as nanopowder for the production of special glasses. We propose Drosophila melanogaster as a useful in vivo model system to study the genotoxic risks associated with NP exposure. In this study, we have conducted a genotoxic evaluation of B 2 O 3 NPs (size average 55.52 ± 1.41 nm) and its ionic form in D. melanogaster. B 2 O 3 NPs were supplied to third instar larvae at concentrations ranging from 0.1-10 mM. Toxicity, intracellular oxidative stress (reactive oxygen species, ROS), phenotypic alterations, genotoxic effect (via the wing somatic mutation and recombination test, SMART), and DNA damage (via Comet assay) were the end-points evaluated. B 2 O 3 NPs did not cause any mutagenic/recombinogenic effects in all tested non-toxic concentrations in Drosophila SMART. Negative data were also obtained with the ionic form. Exposure to B 2 O 3 NPs and its ionic form (at two highest concentrations, 2.5 and 5 mM) was found to induce DNA damage in Comet assay. Additionally, ROS induction in hemocytes and phenotypic alterations were determined in the mouths and legs of Drosophila. This study is the first study reporting genotoxicity data in the somatic cells of Drosophila larvae, emphasizing the importance of D. melanogaster as a model organism in investigating the different biological effects in a concentrationdependent manner caused by B 2 O 3 NPs and its ionic form. The obtained in vivo results contribute to improvement the genotoxicity database on the B 2 O 3 NPs.

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“…By contrast, experimental studies also displayed beneficial effects of boron on reproductive functions. For instance, dietary boron supplementation (40 ppm) in male goats increased sperm output, sperm mobility and enhanced immune and antioxidant defence capacity (Krishnan et al 2019), and a boron-deficient diet produced a negative impact on early embryonic development in rats (Lanoue et al 1998). Inorganic boron compounds may counteract genotoxicity (Marat et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Effects of boron compounds on human reproduction

2020

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Because of the high pKa of boric acid, inorganic borates, when taken up by the human or animal organism, exist in the body almost exclusively in the form of non-dissociated boric acid. Therefore, the variety of inorganic boron compounds is commonly addressed in the toxicological literature as “boron” (B). There is a discussion concerning categorisation of inorganic boron compounds as reproductive toxins. Boron treatment of rats, mice and dogs was dose-dependently associated with testicular toxicity, characterised by inhibited spermiation at lower dose levels and by reduction of epididymal sperm counts at higher dose levels. The NOAEL for such fertility effects of boric acid in male rats (oral feeding, Sprague Dawley strain) was evaluated to be 17.5 mg B/kg bw per day. As far as developmental toxicity is concerned, oral dosing of 9.6 mg B/kg bw daily to female pregnant Sprague Dawley rats remained without effects, with foetal skeletal effects observed at higher doses. Therefore, 9.6 mg B/kg bw (oral dosing) was evaluated as NOAEL for developmental effects of boric acid. The blood level in rats, equivalent to this NOAEL, is 1270 ng B/g. As far as B-exposed humans are concerned, field studies on the effect of boron on human reproduction are possible only in a few boron-rich geographical areas. Published field studies were conducted in China’s Liaoning province, the Argentinian Andes and Western Anatolia/Turkey. Particularly relevant are studies on occupationally B-exposed groups, because the potential exposure to boron is much higher in occupational compared to environmental settings. Comparison of estimated daily B exposure levels in humans and actually measured B blood levels confirms the preference of biomonitoring for exposure assessment in environmental and occupational studies. A boron blood level scaling shows that the levels of high occupational B exposures reported in China and in Turkey are compatible. Compared to the experimental B blood levels at boron-related NOAELs for male fertility and for developmental toxicity in rats, the human blood level means of the highest occupational exposure groups in China and in Turkey are lower by factors of > 4 and > 2, respectively. Basically, concentrations of B within the body that exert reproductive toxicity in humans are not reached under the conditions of human normal handling and use, including conditions of extreme occupational exposures. In consequence, all relevant results of studies into human reproductive toxicity of B are basically negative. Considering the effective doses, there is no scientific contradiction between experimental and human results of B reproductive toxicity.

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Dietary boron supplementation enhances sperm quality and immunity through influencing the associated biochemical parameters and modulating the genes expression at testicular tissue

Cited by 14 publications

References 54 publications

Combination of trehalose and low boron in presence of decreased glycerol improves post‐thawed ram sperm parameters: A model study in boron research

Combination of trehalose and low boron in presence of decreased glycerol improves post‐thawed ram sperm parameters: A model study in boron research

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Effects of boron compounds on human reproduction

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