Dietary black raspberries modulate DNA methylation in dextran sodium sulfate (DSS)-induced ulcerative colitis

Abstract

Our results suggest that dietary BRBs suppress colonic ulceration by correcting promoter hypermethylation of suppressor genes in the colon, as well as in the spleen and bone marrow that systematically regulate inflammation in DSS-induced UC.

The effect of diet on the intestinal epigenome

“…Although the level of supplementation used in these studies was quite high (equivalent to ∼600 g fresh raspberries per day), these results support the conjecture that dietary components can and do modify the colorectal mucosal epigenome in humans, and raise the possibility that dietary modulation of DNA methylation can be harnessed as a strategy for chemoprevention of CRC [83]. In recent studies with animal models, the same group has confirmed that dietary supplementation with black raspberries inhibits colorectal carcinogenesis by partial reactivation of epigenetically silenced Wnt regulatory genes [84,85]. They have also convincingly demonstrated that the anthocyanins, a group of polyphenols present in large quantities in black raspberries, are at least in part responsible for these effects [86].…”

Dietary anthocyanins as a complementary medicinal approach for management of inflammatory bowel disease

“…Male BALB/c mice Oral: 1.62 g/mouse # MDA, # mucosal injury in the ileum [114] Cooked navy and black bean anthocyanins "histological injury score and apoptosis, "serum ORAC, reversed splenic tissue weight increase [164] Black raspberry anthocyanins Fruit DSS-induced UC C57BL/6J male mice Oral: 5% black raspberry diets # Staining of macrophages and neutrophils, # NF-kB p65 nuclear localization in the colon, # ulceration in the mucosa, submucosa and muscularis on day 28, # protein expression of DNMT3B, HDAC1, HDAC2 and MBD2, # HDAC1 mRNA expression in spleen, # mRNA expression of DNMT3B, HDAC1, HDAC2 and MBD2 in bone marrow, "methylation and mRNA expression of dkk3, dkk2 and apc, # b-catenin nuclear localization [155] Black raspberry anthocyanins Dietary anthocyanins as a complementary medicinal approach for management of IBD Review informahealthcare.com from nephropathy [88], antioxidative effects [89,90], antiinflammatory activities [91], antiobesity [92], antidiabetic, vision improvement properties, inhibition of carcinogenesis and mutagenesis [93]. An in vivo research showed that wildblueberry-enriched diet improved the proinflammatory status associated with metabolic syndrome in the obese Zucker rat with decreased liver expression of NF-kB, C-reactive protein, IL-6 and increased adiponectin expression [94].…”

“…Wang et al demonstrated that 5% black raspberry diets possess anti-inflammatory effects by reduction of NF-kB p65 nuclear localization in the colon, staining of macrophages and neutrophils, ulceration in the mucosa, submucosa and muscularis in DSS-induced UC [155]. It also decreased protein expression of deoxyribonucleic acid methyltransferase-3B, histone deacetylase (HDAC)-1, HDAC2 and methyl-binding domain-2, HDAC1 mRNA expression in spleen, mRNA expression of deoxyribonucleic acid methyltransferase 3B, HDAC1, HDAC2 and methyl-binding domain-2 in bone marrow, methylation and mRNA expression of dkk3, dkk2 and apc, and b-catenin nuclear localization [155].…”

“…Wang et al demonstrated that 5% black raspberry diets possess anti-inflammatory effects by reduction of NF-kB p65 nuclear localization in the colon, staining of macrophages and neutrophils, ulceration in the mucosa, submucosa and muscularis in DSS-induced UC [155]. It also decreased protein expression of deoxyribonucleic acid methyltransferase-3B, histone deacetylase (HDAC)-1, HDAC2 and methyl-binding domain-2, HDAC1 mRNA expression in spleen, mRNA expression of deoxyribonucleic acid methyltransferase 3B, HDAC1, HDAC2 and methyl-binding domain-2 in bone marrow, methylation and mRNA expression of dkk3, dkk2 and apc, and b-catenin nuclear localization [155]. Moreover, another in vivo study demonstrated that oral administration of 5 or 10% of freeze-dried black raspberry powder can diminish proinflammatory cytokines such as TNF-a, IL-1b, p-Ikba within the colonic tissue as well as levels of colonic COX-2 and plasma prostaglandin E2, which confirms beneficial effects of black raspberry in DSS-induced UC [156].…”

Linking Immunity, Epigenetics, and Cancer in Inflammatory Bowel Disease

“…Dietary intake of black raspberries may suppress colonic ulceration by correcting promoter hypermethylation of homeostatic genes that systematically regulate inflammation in a model of DSS-induced colitis. 220 Likewise, inhibition of DNA methylation in DSS-induced colitis results in disease exacerbation, whereas folate supplementation to promote methylation partially ameliorates the severity of colitis. 201 Dietary folate did not significantly affect the intestinal microbiome and inflammation in DSS-induced colitis in another study.…”