Dietary betaine supplementation in hens modulates hypothalamic expression of cholesterol metabolic genes in F1 cockerels through modification of DNA methylation

Idriss, Abdulrahman A.; Hu, Yun; Hou, Zhen; Hu, Yan; Sun, Qinwei; Omer, Nagmeldin A.; Abobaker, Halima; Ni, Yingdong

doi:10.1016/j.cbpb.2017.12.001

Cited by 20 publications

(14 citation statements)

References 39 publications

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“…Here, we found that addition of methyl donors, betaine, had a similar effect on DNA methylation status on BDNF promoters and the mRNA expression levels among the chicken offsprings. Even within more generations, the maternal effect of betaine on hypothalamic transcription of BDNF is similar to our previous work ( Idriss et al., 2018 ). These findings, including associated with the modification of the cholesterol metabolism in chicken brain, could be a new insight of methyl donor effect on BDNF mRNA transcription.…”

Section: Discussionsupporting

confidence: 88%

“…Additionally, data about NPY mRNA level was agreed with our results that suggested epigenetic modifications could contribute to the altered gene expression of the NPY and POMC in hypothalami leading to development of obesity in rats ( Mahmood et al., 2013 ). Otherwise, a significant upregulation of NPY mRNA expression was observed in our previous chicken model ( Idriss et al., 2018 ). The maternal effect of betaine on hypothalamic transcription of NPY was obviously reversed in the different generations.…”

Section: Discussionmentioning

confidence: 55%

“…Our result was inconsistent with of CRH mRNA, while agreed with increased CRH gene expression in those reported phenomena ( Xu et al., 2014 ). According to our previous work ( Idriss et al., 2018 ), the maternal effect of betaine on hypothalamic transcription of CRH, like BDNF, was sustained through generations. Otherwise, more experimental evidences are really needed to explain these interesting findings.…”

Section: Discussionmentioning

confidence: 86%

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Fetal betaine exposure modulates hypothalamic expression of cholesterol metabolic genes in offspring cockerels with modification of promoter DNA methylation

Idriss

Sun

et al. 2020

Poultry Science

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In documents, maternal betaine modulates hypothalamic cholesterol metabolism in chicken posthatchings, but it remains unclear whether this effect can be passed on by generations. In present study, eggs were injected with saline or betaine at 2.5 mg/egg, and the hatchlings (F1) were raised under the same condition until sexual maturation. Both the control group and the betaine group used artificial insemination to collect sperm from their cockerels. Fertilized eggs were incubated, and the hatchlings of the following generation (F2) were raised up to 64 D of age. F2 cockerels in betaine group showed significantly ( P < 0.05) lower body weight, which was associated with significantly decreased ( P < 0.05) hypothalamic content of total cholesterol and cholesterol ester. Concordantly, hypothalamic expression of cholesterol biosynthetic genes, SREBP2 and HMGCR , were significantly downregulated ( P < 0.05), together with cholesterol conversion-related and excretion-related genes, CYP46A1 and ABCA1 . These changes coincided with a significant downregulation in mRNA expression of regulatory neuropeptides including brain-derived neurotrophic factor, neuropeptide Y, and corticotropin-releasing hormone. Moreover, genes involved in methyl transfer cycle were also modified. Betaine homocysteine methyltransferase ( P < 0.05) was downregulated, yet DNA methyltransferase1 tended to be upregulated ( P = 0.06). S-adenosyl methionine/S-adenosylhomocysteine ratio was higher in the hypothalamus of betaine-treated F2 cockerels, which was associated with significantly modified CpG methylation on the promoter of those affected genes. These results suggested that betaine might regulate central cholesterol metabolism and hypothalamic expression of genes related to brain function by altering promoter DNA methylation in F2 cockerels.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 86%

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Fetal betaine exposure modulates hypothalamic expression of cholesterol metabolic genes in offspring cockerels with modification of promoter DNA methylation

Idriss

Sun

et al. 2020

Poultry Science

Self Cite

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“…Effect of B may be extending to progeny through egg. Idriss et al (2018) indicated that feeding B to the hens modifies hypothalamic expression of genes complicated in cholesterol metabolism and brain tasks in F1 cockerels through modification of promoter DNA methylation. Hu et al (2015) indicated that epigenetic mechanisms including DNA and histone methylations can regulate hepatic cholesterol metabolism in chicks by in ovo injection of B.…”

Section: Resultsmentioning

confidence: 99%

Using Feed Additives to Produce Functional Eggs in Fayoumi Hens.

Allah¹

2020

jwpr

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Lately human have become more apprehensive for the health and their food relationship. Egg considered cheap source of animal protein. Eggs are rich in various essential nutrients that contribute to the quality of human diet. But its cholesterol can contributes with some human serious disease. The current study examines the hypothesis that assumed addition of antioxidant such as CAX, SS, B or their mixtures to the diet can produce functional egg from Fayoumi hens at late phase of egg production. A number of 168 Fayoumi hens (46weeks of age) were randomly assigned into 8 dietary groups as follows: Basal diet alone or with CAX (6 ppm), SS (0.5 g/kg), B (1 g/kg), CAX+SS, CAX+B, SS+B, and CAX+SS+B separately. Forty eight eggs (6 per each group) were analyzed for estimating cholesterol and total antioxidant capacity. Egg of hens fed a combination of CAX+SS+B which had the best total antioxidant capacity value, while the CAX group recorded the best lowest cholesterol value compared to other groups (P < 0.05). It could be concluded that basal diet supplemented with CAX, SS, B alone or with mixture of them may have lowering effect on yolk total cholesterol. This could lead to produce functional eggs which have positive effects on human health and favorable for those suffering from heart syndromes.

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“…DNA methylation level was analysis by an multiplex PCR and next-generation sequencing-based Targeted CpG methylation analysis method--MethylTarget™ (Genesky Biotechnologies Inc., Shanghai, China). The validity and reliability of this method has been previously reported (22)(23)(24). Speci cally, CpG islands located in the promoter of genes of interest were selected according to the following criteria: (1) 200 bp minimum length; (2) above 50% GC content; (3) above 0.6 ratio of observed/expected CpG.…”

Section: Dna Methylation Analysismentioning

confidence: 99%

Interaction between early-life pet exposure and methylation pattern of ADAM33 on Allergic Rhinitis among a cohort of kindergartens in China

Zhang

Tan

Qian

et al. 2020

Preprint

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Background Recent research has pointed out the important role of epigenetic modifications in the development and persistence of Allergic Rhinitis (AR), especially DNA methylation. We investigated whether AR susceptibility genes are epigenetically regulated, and whether methylation modulation of these genes in response to early-life environment could be a molecular mechanism underlying the risk of AR in a cohort of kindergartens in China. Methods Peripheral blood mononuclear cell (PBMCs) samples were collected from 130 kindergarten patients, aged 3–6 years with AR and 154 matched healthy controls to detect promoter methylation in 25 AR susceptibility genes with the MethylTarget approach. Methylation level was compared for each CpG site, each amplified region, and each gene. In addition, the relationship among DNA methylation, early-life environment risk factors and AR were assessed. Results Maternal allergic history (P = 0.0390) and exposure to pet (P = 0.0339) were significantly associated with increase AR risk. Differential methylation analyses were successfully performed for 507 CpG sites, 34 amplified regions and 17 genes and significant hypomethylation was observed in the promoter region of ADAM33 in AR patients [multiple test-corrected (FDR) P-value < 0.05]. Spearman correlation analysis revealed that the hypermethylation of ADAM33 was significantly associated with lower serum total eosinophil counts (Spearman's ρ: -0.187, P-value = 0.037). According to the results of the multiple regression analysis, after adjusting for cofounders, the interaction of early-life pet exposure and methylation level of ADAM33 increased the risk of AR 1.423 times in kindergartens (95% CI = 0.0290–4.109, P-value = 0.005). Conclusion This study provides evidence that early-life pet exposure and low methylation level of ADAM33 increase AR risk in kindergartens, and the interaction between pet exposure and methylation level of ADAM33 may play an important role in the development of AR.

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Dietary betaine supplementation in hens modulates hypothalamic expression of cholesterol metabolic genes in F1 cockerels through modification of DNA methylation

Cited by 20 publications

References 39 publications

Fetal betaine exposure modulates hypothalamic expression of cholesterol metabolic genes in offspring cockerels with modification of promoter DNA methylation

Fetal betaine exposure modulates hypothalamic expression of cholesterol metabolic genes in offspring cockerels with modification of promoter DNA methylation

Using Feed Additives to Produce Functional Eggs in Fayoumi Hens.

Interaction between early-life pet exposure and methylation pattern of ADAM33 on Allergic Rhinitis among a cohort of kindergartens in China

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