Dietary Baicalin Zinc Supplementation Alleviates Oxidative Stress and Enhances Nutrition Absorption in Deoxynivalenol Challenged Pigs

Abstract

Background: Deoxynivalenol contamination is increasing worldwide, presenting great challenges to food security and causing great economic losses in the livestock industry. Objective: This study was conducted to determine the protective effect of baicalin zinc as a dietary supplement on pigs fed with a deoxynivalenol-contaminated diet. Method: A total of 40 weaned pigs (21 d of age; 6.13 ± 0.42 kg average BW) were randomly assigned (10 pigs/group) to 4 dietary treatments: basal diet (Con group), basal diet + 4 mg/kg DON (DON group), basal diet + 5 g/kg BZN (BZN group), and basal diet + 5 g/kg BZN + 4 mg/kg DON (DBZN group) for a 14-d period. Seven randomly selected pigs from each treatment were killed for blood and tissue sampling. Results: The results showed that piglets challenged with DON exhibited significantly reduced levels of ADG, ADFI, and F/G (p <0.05). BZN supplemented diets significantly suppressed the protein expression of p-Nrf2, p-NF-kB, and HO-1 in the intestine of DON challenged piglets (p < 0.05). In liver, DON markedly increased the mRNA expression of P70S6K and HSP70 in piglets fed the basal diet, but significantly reduced that of HO-1, NQO-1, NF-kB, AMPKα2 and HSP70 in piglets fed the BZN supplemented diet (p < 0.05). Dietary supplementation with BZN markedly increased the T-AOC level of serum in weaned piglets (p < 0.05). In jejunum, dietary supplementation with BZN activated the mRNA expression of ZIP4 in piglets (p < 0.05), BZN supplementation significantly suppressed the activity of sucrose and increased the protein concentration in chyme (p < 0.05). Conclusion: BZN can play a protective role by reducing oxidative stress and enhancing nutrition absorption in pigs fed DON-contaminated diets.

Lactobacillus salivarius, a Potential Probiotic to Improve the Health of LPS-Challenged Piglet Intestine by Alleviating Inflammation as Well as Oxidative Stress in a Dose-Dependent Manner During Weaning Transition

“…Upregulation of antioxidant enzymes (SOD, CAT, and glutathione S-transferase) resulted from L. plantarum AS1 in the colon of cancer-bearing subjects suggests an anticancer benefit of this probiotic through its antioxidant attributes (57). In case of piglets, since previous studies have shown that weaning or diet contamination could cause the disordered intestine of piglets where the occurrence and the development of oxidative stress are common (58,59). Dietary supplement including some chemical complexes or microorganisms have been reported to have positive influence on the oxidative stress (59).…”

“…In case of piglets, since previous studies have shown that weaning or diet contamination could cause the disordered intestine of piglets where the occurrence and the development of oxidative stress are common (58,59). Dietary supplement including some chemical complexes or microorganisms have been reported to have positive influence on the oxidative stress (59). For instance, Baicalin-zinc or -copper is capable of exerting antioxidant capability in deoxynivalenol-challenged piglets, which is reflected in the reduction of HO-1, NQO-1, NF-κB (60, 61).…”

Baicalin inhibits Salmonella typhimurium‐induced inflammation and mediates autophagy through TLR4/MAPK/NF‐κB signalling pathway

“…It was indicated that Baicalin alleviates macrophage damage caused by bacteria. Zha had been reported that Baicalin Zinc or Baicalin‐Copper has anti‐oxidant effects in Deoxynivalenol Challenged Pigs 23,24 . Baicalin is a key flavonoid found in the root of Scutellaria baicalensis Georgi , and it is known to have different biological activities.…”

“…Zha had been reported that Baicalin Zinc or Baicalin-Copper has anti-oxidant effects in Deoxynivalenol Challenged Pigs. 23,24 Baicalin is a key flavonoid found in the root of Scutellaria baicalensis Georgi, and it is known to have different biological activities. Combined with the results of the current study, it can be fully confirmed that Baicalin has anti-oxidant effects.…”

“…It has poor water solubility (91 μg/mL) and low oral bioavailability (2.2 ± 0.2%) 20,21 . Baicalin and its flavonoids have been reported to have various pharmacological activities, such as anti‐oxidant, 22‐24 antimicrobial, 25,26 anti‐inflammatory and anticancerous effects 27,28 . Baicalin has a strong ability to scavenge reactive oxygen species (ROS) and protects cells from inflammatory damage induced by ultraviolet B (UVB) through TLR pathway and inhibits autophagy induced by influenza A virus subtype H3N2 29,30 .…”