Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck

Qin, Simeng; Zhang, Keying; Applegate, T. J.; Ding, Xuemei; Bai, Shiping; Luo, Yuheng; Wang, Jianping; Peng, Huanwei; Su, Zuowei; Xuan, Yue; Zeng, Qiufeng

doi:10.1017/s0007114519002319

Cited by 29 publications

(48 citation statements)

References 62 publications

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“…The symbiotic bacteria compete to inhibit pathogens from contacting the surface of epithelial cells of the intestine and maintain the integrity of the barrier [ 17 ]. At the same time, some special symbiotic bacteria can produce SCFAs to maintain intestinal health [ 18 ]. The mycotoxins can destroy the barrier function of intestinal epithelial cells in both animals and humans and can cause intestinal lesions [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Astaxanthin Alleviates Ochratoxin A-Induced Cecum Injury and Inflammation in Mice by Regulating the Diversity of Cecal Microbiota and TLR4/MyD88/NF-κB Signaling Pathway

Chen

Zhao

Jiao

et al. 2021

Oxidative Medicine and Cellular Longevity

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Ochratoxin A (OTA) is a common environmental pollutant found in a variety of foods and grains, and excessive OTA consumption causes serious global health effects on animals and humans. Astaxanthin (AST) is a natural carotenoid that has anti-inflammatory, antiapoptotic, immunomodulatory, antitumor, antidiabetes, and other biological activities. The present study is aimed at investigating the effects of AST on OTA-induced cecum injury and its mechanism of action. Eighty C57 mice were randomly divided into four groups, including the control group, OTA group (5 mg/kg body weight), AST group (100 mg/kg body weight), and AST intervention group (100 mg/kg body weight AST+5 mg/kg body weight OTA). It was found that AST decreased the endotoxin content, effectively prevented the shortening of mouse cecum villi, and increased the expression levels of tight junction (TJ) proteins, consisting of occludin, claudin-1, and zonula occludens-1 (ZO-1). AST increased the number of goblet cells, the contents of mucin-2 (MUC2), and defensins (Defa5 and β-pD2) significantly, while the expression of mucin-1 (MUC1) decreased significantly. The 16S rRNA sequencing showed that AST affected the richness and diversity of cecum flora, decreased the proportion of lactobacillus, and also decreased the contents of short-chain fatty acids (SCFAs) (acetate and butyrate). In addition, AST significantly decreased the expression of TLR4, MyD88, and p-p65, while increasing the expression of p65. Meanwhile, the expression of inflammatory factors including TNF-α and INF-γ decreased, while the expression of IL-10 increased. In conclusion, AST reduced OTA-induced cecum injury by regulating the cecum barrier function and TLR4/MyD88/NF-κB signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Astaxanthin Alleviates Ochratoxin A-Induced Cecum Injury and Inflammation in Mice by Regulating the Diversity of Cecal Microbiota and TLR4/MyD88/NF-κB Signaling Pathway

Chen

Zhao

Jiao

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…At the same crude fiber levels, the starter broilers fed fiber from soy hulls had better growth performance, intestinal histomorphology, and nutrient digestibility compared with the birds fed fiber from purified cellulose (Tejeda and Kim, 2020). Recently, when ducks were fed with diets containing high levels of resistant starch, the gut barrier was improved by enhancing intestinal morphology and barrier markers expression, modulating the microbiota composition, and attenuating inflammatory markers (Qin et al, 2019(Qin et al, , 2020). In the aforementioned two studies, soy hulls contained many fibrous components out of category of crude fiber, and resistant starch was out of category of crude fiber.…”

Section: Introductionmentioning

confidence: 99%

Effects of Total Dietary Fiber on Cecal Microbial Community and Intestinal Morphology of Growing White Pekin Duck

Hao

Shen

et al. 2021

Front. Microbiol.

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The current study was to investigate the effects of total dietary fiber (TDF) on growth performance, cecal structure, cecal microbial community, and short-chain fatty acids (SCFAs) profiles in the cecum of growing White Pekin ducks. A total of 108 male Pekin ducks of 14-days-old were randomly allocated and fed diets containing 12.4, 14.7, and 16.2% TDF for 35 days. Each dietary treatment consisted of six replicates with six birds each. The results showed that 14.7 and 16.2% TDF treatments promoted growth performance relative to 12.4% TDF treatments (P < 0.05). A total of 14.7 and 16.2% TDF treatments significantly elevated villus height, the ratio of villus height to crypt depth and muscle layer thickness of cecum, and lowered crypt depth compared with 12.4% TDF treatment (P < 0.05). Simultaneously, 14.7 and 16.2% TDF treatments up-regulated Claudin-1 mRNA expression of barrier genes in the cecum compared with 12.4% TDF (P < 0.05). Butyrate-producing bacteria like Oscillopiraceae affiliating to the phyla Firmicutes were observed as a biomarker in the 16.2% TDF. Higher concentration of butyrate in the cecum was obtained in the 14.7% TDF compared with 12.4 and 16.2% TDF (P < 0.05). The concentrations of isobutyrate, valerate, and isovalerate in the cecum were significantly increased in the 16.2% TDF compared with 12.4 and 14.7% TDF (P < 0.05). Meanwhile, the abundance of genus UCG-005 and Enterococcus was positive correlations with isobutyrate and valerate (P < 0.05). However, the concentration of propionate in the cecum significantly decreased in 14.7 and 16.2% TDF treatments relative to 12.4% TDF treatments (P < 0.05). In summary, increasing TDF levels improved growth performance, cecal histomorphology, and barrier function of meat ducks and it might be mediated by the changes of microbiota communities, especially bloom of SCFAs-producing bacteria, which facilitated the interaction between intestinal mucosa and microbiota.

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“…The enzyme activities of phytase and cellulase in cecal digesta were determined spectrophotometrically with commercial kits purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, People's Republic of China) as per the instructions of the manufacturer. The cecal SCFA content was determined using the method described in our previous study ( Qin et al., 2020 ). Briefly, approximately 0.5 g of cecal digesta was diluted with 2 mL ultrapure water, mixed and centrifuged (3,000 × g , 15 min).…”

Section: Methodsmentioning

confidence: 99%

“…In Pekin ducks, previous studies by Qin et al. (2019 , 2020) found ducks can ferment dietary resistant potato starch ( RPS ) at 12% dosage to produce SCFA by modulating cecal microbiota, which subsequently enhanced the intestinal barrier function, as well as improved the growth performance. Yin et al.…”

Section: Introductionmentioning

confidence: 99%

Dietary resistant potato starch improves growth performance and feather development in Pekin ducks fed a low phosphorus diet

Zhang

Bai

et al. 2021

Poultry Science

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This study investigated whether dietary resistant potato starch ( RPS ) inclusion could ameliorate the negative impact of a low nonphytate phosphorus ( nPP ) diet on growth performance, feather growth, feather follicles ( FF ) development, and carcass traits by improving nutrient utilization and cecal microbiome fermentation capacity in Pekin ducks. The experiment was performed with a 2 × 2 randomized block design with 2 levels of RPS (0 or 12%) and 2 levels of nPP (low or normal, low: 0.22% at 1–14 d and 0.18% at 15–35 d of age; normal: 0.40% at 1–14 d and 0.35% at 15–35 d of age) for a total of 4 treatments, each with 8 replicate pens per treatment of 12 birds per pen. As regards growth performance and carcass traits, RPS inclusion markedly increased ( P < 0.05) BW of 14 and 35 d, BWG and FI of 1–14 d, 15–35 d, and 1–35 d as well as abdominal fat and breast meat percentage of 35 d in ducks fed low nPP diets; moreover, RSP inclusion significantly reduced ( P < 0.05) mortality in ducks fed low nPP diets. As regards feather growth and follicles development of 35 d, RPS inclusion significantly increased ( P < 0.05) the fourth primary feather length, absolute feather weight, and the density of primary FF in the back skin in ducks fed low nPP diets. In regard to nutrition utilization, RPS supplementation significantly increased ( P < 0.05) the availability of DM, CP, and energy, as well as dietary AME at 35 d of age in ducks fed low nPP diets. However, RPS supplementation had no effect ( P > 0.05) on the concentration of cecal short-chain fatty acids and the activities of cecal phytase and cellulase in ducks fed low nPP diets. These results indicate that RPS can improve nutrient availability to ameliorate the negative effects on performance and feather development caused by a low nPP diet in Pekin ducks.

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Dietary administration of resistant starch improved caecal barrier function by enhancing intestinal morphology and modulating microbiota composition in meat duck

Cited by 29 publications

References 62 publications

Astaxanthin Alleviates Ochratoxin A-Induced Cecum Injury and Inflammation in Mice by Regulating the Diversity of Cecal Microbiota and TLR4/MyD88/NF-κB Signaling Pathway

Astaxanthin Alleviates Ochratoxin A-Induced Cecum Injury and Inflammation in Mice by Regulating the Diversity of Cecal Microbiota and TLR4/MyD88/NF-κB Signaling Pathway

Effects of Total Dietary Fiber on Cecal Microbial Community and Intestinal Morphology of Growing White Pekin Duck

Dietary resistant potato starch improves growth performance and feather development in Pekin ducks fed a low phosphorus diet

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