Dietary acidification enhances phosphorus digestibility but decreases H+/K+-ATPase expression in rainbow trout

Sugiura, Shozo; Roy, P.K.; Ferraris, Ronaldo P.

doi:10.1242/jeb.02436

Cited by 68 publications

(66 citation statements)

References 32 publications

(37 reference statements)

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“…Due to carbonate salts, the pH of the intestine is alkaline in nature, creating a pH of about 8.5 which supports our findings [27]. Alkaline nature of extracellular protease and amylase produced by different bacterial strains has also been reported by many authors [13,28].…”

Section: Characterization Of Purified Protease and Amylasesupporting

confidence: 92%

Purification and Characterization of Extracellular Protease and Amylase Produced by the Bacterial Strain, Corynebacterium alkanolyticum ATH3 Isolated from Fish Gut

et al. 2015

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The use of enzymes in different industrial sectors increased significantly due to huge industrialization. Different types of protease and amylase are randomly used in industries including food, textile, and paper. For this purpose, purification of extracellular protease and amylase produced by the bacterium, Corynebacterium alkanolyticum ATH3 (Acc. No. JX656749) isolated from the distal intestine of a freshwater fish, Anabas testudineus, was carried out using column chromatography. The specific activity of protease and amylase significantly increased with each step of purification and finally became 93.73 and 88.1 U/mg protein with a purification fold 26.03 and 44.94, respectively. The sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed molecular weight of purified protease and amylase was ∼17 and ∼28 kDa, respectively. To the authors' knowledge, this is the first report of ∼17 kDa protease and ∼28 kDa amylase from the bacterial strain C. alkanolyticum ATH3. Further, enzyme activity was also evidenced by zymography analysis. The enzymes acted optimally at pH 7.5-8.0 and temperature 35-45 • C, respectively. So, due to cheapest source, these two enzymes are very important for various purposes in industrial sectors.Keywords Fish gut bacteria · Protease and amylase purification · SDS-PAGE · Zymography analysis · pH and temperature sensitivity B Arun K. Ray

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Section: Characterization Of Purified Protease and Amylasesupporting

confidence: 92%

Purification and Characterization of Extracellular Protease and Amylase Produced by the Bacterial Strain, Corynebacterium alkanolyticum ATH3 Isolated from Fish Gut

et al. 2015

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“…Upon stimulation, these cells secrete HCl into the stomach lumen to facilitate digestion, as well as HCO 3 -into the extracellular fluid compartment in order to maintain intracellular pH. A K + -stimulated, H + -ATPase is responsible for the apical H + secretion, and has been identified in oxynticopeptic cells of elasmobranchs (Smolka et al, 1994) as well as those of the rainbow trout (Sugiura et al, 2006). Although there is a vigorous secretion of gastric acid at this time, the pH of the stomach fluid actually increases substantially due to the buffering action of the ingested food (Sugiura et al, 2006;Bucking and Wood, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…A K + -stimulated, H + -ATPase is responsible for the apical H + secretion, and has been identified in oxynticopeptic cells of elasmobranchs (Smolka et al, 1994) as well as those of the rainbow trout (Sugiura et al, 2006). Although there is a vigorous secretion of gastric acid at this time, the pH of the stomach fluid actually increases substantially due to the buffering action of the ingested food (Sugiura et al, 2006;Bucking and Wood, 2008). As a Cl -/HCO 3 -exchanger is believed to be responsible for the basolateral HCO 3 -export and Cl -entry, the net transfer of HCl to the stomach can lead to a reduction in plasma [Cl -] that has been correlated with an alkaline tide in toads (Busk et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

The alkaline tide and ammonia excretion after voluntary feeding in freshwater rainbow trout

Bucking

Wood

2008

Journal of Experimental Biology

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SUMMARYWe investigated the potential acid-base and nitrogenous waste excretion challenges created by voluntary feeding in freshwater rainbow trout, with particular focus on the possible occurrence of an alkaline tide (a metabolic alkalosis created by gastric HCl secretion during digestion). Plasma metabolites (glucose, urea and ammonia) were measured at various time points before and after voluntary feeding to satiation (approximately 5% body mass meal of dry commercial pellets), as was the net flux of ammonia and titratable alkalinity to the water from unfed and fed fish. Arterial blood, sampled by indwelling catheter, was examined for post-prandial effects on pH, plasma bicarbonate and plasma CO 2 tension. There was no significant change in plasma glucose or urea concentrations following feeding, whereas plasma ammonia transiently increased, peaking at threefold above resting values at 12 h after the meal and remaining elevated for 24 h. The increased plasma ammonia was correlated with an increase in net ammonia excretion to the water, with fed fish significantly elevating their net ammonia excretion two-to threefold between 12 and 48 h post feeding. These parameters did not change in unfed control fish. Fed fish likewise increased the net titratable base flux to the water by approximately threefold, which resulted in a transition from a small net acid flux seen in unfed fish to a large net base flux in fed fish. Over 48 h, this resulted in a net excretion of 13·867·μmol·kg -1 more base to the external water than in unfed fish. The arterial blood exhibited a corresponding rise in pH (between 6 and 12 h) and plasma bicarbonate (between 3 and 12 h) following feeding; however, no respiratory compensation was observed, as Pa CO 2 remained constant. Overall, there was evidence of numerous challenges created by feeding in a freshwater teleost fish, including the occurrence of an alkaline tide, and its compensation by excretion of base to the external water. The possible influence of feeding ecology and environmental salinity on these challenges, as well as discrepancies in the literature, are discussed.

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“…The main form of dietary P in fish diet is hydroxyapatite or bone phosphate which requires strong acidity to be solubilized in the stomach for subsequent absorption in the intestine (Sugiura et al, 2006). This may be the reason why P in fishmeal is well-digested by mammals (Soares Jr, 1995) which have very low post-prandial stomach pH of 2 or less (Benne & Levy, 2000) and poorly digested by fish (Cho & Bureau, 2001).…”

Section: Fluctuation Of Post-prandial Plasma Mineral Level (Asda mentioning

confidence: 99%

FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION

Laining

Rachmansyah

Lideman³

et al. 2010

Indonesian Aquaculture Journal

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In order to investigate the phytic acid degradation in the gut of post juvenile Japanese flounder, indirect method was carried out by measuring the pre-prandial and postprandial plasma mineral and alkaline phosphatase (ALP) level as well as liver phosphorus content. The experiment was designed into a Randomized Block in which experiment units were grouped according to sampling days at 10, 20 and 30 days of feeding time. Experimental diets contained three levels of dietary inorganic phosphorus at 0.0; 0.25 and 0.5% combined with two levels of dietary phytase at 0 and 2000 FTU/kg diet. Juvenile Japanese flounder (IBW = 36.2 g) were randomly distributed into 6 tanks of a 200 L capacity with density of 15 fish/tank. Blood sampling was carried out at 0 hour (before feeding or pre-prandial) and at 1, 3, 6 and 12 hour post feeding (post-prandial) time in three sampling days, respectively. Plasma was measured for mineral and ALP levels, while liver was analyzed for P content. The observation showed that fish fed without both dietary IP and phytase supplements had the lowest postprandial plasma IP, Mg and ALP levels during 12-h postprandial period. Plasma IP level at 6-h post-feeding in groups fed dietary 0.25 and 0.5% IP were significant higher when diet supplemented with phytase than those without phytase supplement. Peak level of plasma IP in fish fed 0.25% IP was similar to fish fed 0.5% with the presence of dietary phytase. At 1 and 3-h post-feeding, plasma Ca level increased in all groups, but significant difference was only observed between group fed diet without both dietary IP and phytase and other groups. Similar to plasma IP level, peak of plasma Mg and ALP concentration occurred in fish fed 0.25% IP together with phytase, and did not significantly differ from fish fed with 0.5% IP even when phytase was included in diet.

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Dietary acidification enhances phosphorus digestibility but decreases H+/K+-ATPase expression in rainbow trout

Cited by 68 publications

References 32 publications

Purification and Characterization of Extracellular Protease and Amylase Produced by the Bacterial Strain, Corynebacterium alkanolyticum ATH3 Isolated from Fish Gut

Purification and Characterization of Extracellular Protease and Amylase Produced by the Bacterial Strain, Corynebacterium alkanolyticum ATH3 Isolated from Fish Gut

The alkaline tide and ammonia excretion after voluntary feeding in freshwater rainbow trout

FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION

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