Diet, Trimethylamine Metabolism, and Mitochondrial DNA: An Observational Study

Bordoni, Laura; Malinowska, Anna M.; Petracci, Irene; Szwengiel, Artur; Gabbianelli, Rosita; Chmurzyńska, Agata

doi:10.1002/mnfr.202200003

Cited by 6 publications

(2 citation statements)

References 107 publications

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“…Thus, it was not possible to adjust results for different dietary patters. Nevertheless, the impact of diet on mtDNA copy number and methylation has been investigated in another study from our group [87].…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA in Visceral Adipose Tissue in Severe Obesity: From Copy Number to D-Loop Methylation

Bordoni¹,

Perugini²,

Petracci³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Peripheral alterations of mitochondrial DNA copy number (mtDNAcn) in obesity and associated co-morbidities have been previously shown. Furthermore, the possibility that methylation could occur in the mtDNA (in particular in the displacement loop, D-Loop) and regulate its functions has been raised. However, limited data about mtDNA methylation in adipose tissue are currently available. Since a strict crosstalk between the nucleus and mitochondria exists, especially in terms of the one-carbon cycle (that supports methylation reactions in the cell), we investigated methylation in selected areas of the mitochondrial and nuclear DNA and their expression in visceral adipose tissue (VAT) samples of patients with severe obesity. Methods: VAT biopsies were collected from surgery patients to isolate DNA and RNA. Gene expression and mtDNAcn were assessed through qPCR. DNA methylation in both nuclear and mitochondrial areas were determined through bisulfite pyrosequencing. Results: Methylation levels of the mtDNA were only marginally associated with the obesity degree (higher D-Loop methylation in severe obesity) and were not correlated with mtDNAcn. A significant correlation between D-Loop methylation and LINE-1 methylation was observed in VAT samples, and this was independent from the obesity degree. A progressive reduction of mtDNAcn and increase in NRF1 expression levels were measured in VAT in severe obesity. NRF1 expression was directly correlated with PPARG and MTHFR expression levels, while mtDNAcn was associated to TFAM expression. The correlation between mtDNAcn and TFAM expression was affected by the obesity status. Conclusions: This evidence supports the hypothesis that mtDNA alterations occur in obesity and a complex dynamic correlation between mitochondrial and nuclear DNA methylation exists, highlighting the need for further investigations.

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Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA in Visceral Adipose Tissue in Severe Obesity: From Copy Number to D-Loop Methylation

Bordoni¹,

Perugini²,

Petracci³

et al. 2022

Front. Biosci. (Landmark Ed)

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“…For this reason, a targeted approach to epigenetic age measurement might facilitate the translation of this course of research, recruitment procedure and inclu-169 sion/exclusion criteria has been previously published 170 [30][31][32]. The mean age of the recruited cohort is 171 38.2 ± 4.9.…”

Section: Introductionmentioning

confidence: 99%

Biological age and diet: Measuring the impact of lifestyle on a 6CpG-epigenetic clock

Bordoni

Malinowska

Petracci

et al. 2022

NHA

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BACKGROUND: Changes in DNA methylation along the life have been documented, and environmental exposures (including diet, physical activity and smoking) can accelerate or decelerate this process. The epigenetic clock estimates the biological age of an individual measuring methylation patterns in specific areas of its genome. Recently, a new epigenetic clock based on 6 CpGs has been proposed, with high potential to become an easy accessible tool able to measure the epigenetic age (EA) of an individual. OBJECTIVES: This study aims to validate the 6 CpG epigenetic clock comparing it with other biomarkers of aging such as telomere length (TL) and methylation in the long interspersed nuclear elements (LINE-1). The impact of life-style associated factors on these molecular marks has been evaluated. METHODS: 200 healthy participants having extreme dietary patterns (healthy vs western diet) were selected. Dietary intakes, body composition, physical activity level and smoking has been assessed. DNA extracted from whole blood was used to measure the 6CpG-EA, TL and LINE-1 methylation levels. RESULTS: 6CpG-EA was positively correlated with chronological age (r = 0.591; p = 7.2*10 - 20) and negatively with TL (r = –0.150; p = 0.040) and LINE-1 methylation (r = –0.240; p = 0.001). Despite no significant associations were detected with the overall diet quality (HEI), 6CpG-EA was correlated with dietary intakes of nutrients involved in the one-carbon (1 C) metabolism, especially in the western diet group. CONCLUSION: These results support the 6CpG epigenetic clock as an easy accessible tool to estimate biological age, in accordance with other molecular markers of aging, and suggest that EA can be modulated by micronutrients involved in the 1 C metabolism.

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The potential role of environmental factors in modulating mitochondrial DNA epigenetic marks

Ryva

Zhou

Goodrich

et al. 2023

Hormones and Epigenetics

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Diet, Trimethylamine Metabolism, and Mitochondrial DNA: An Observational Study

Cited by 6 publications

References 107 publications

Mitochondrial DNA in Visceral Adipose Tissue in Severe Obesity: From Copy Number to D-Loop Methylation

Mitochondrial DNA in Visceral Adipose Tissue in Severe Obesity: From Copy Number to D-Loop Methylation

Biological age and diet: Measuring the impact of lifestyle on a 6CpG-epigenetic clock

The potential role of environmental factors in modulating mitochondrial DNA epigenetic marks

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