Diet supplemented with pancreatic-like enzymes of microbial origin restores the hippocampal neuronal plasticity and behaviour in young pigs with experimental exocrine pancreatic insufficiency

Goncharova, Kateryna; Ушакова, Г. А.; Kovalenko, T. M.; Osadchenko, I.; Skibo, G. G.; Pierzynowski, Stefan

doi:10.1016/j.jff.2015.01.014

Cited by 2 publications

(4 citation statements)

References 28 publications

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“…Treated pigs became more alert, playful, and interactive, suggesting improvement in overall health (data not shown). This behavioral observation was similar to the observed behavior of healthy pigs, and very different from lethargic and cachexic untreated EPI pigs [ 7 , 24 ].…”

Section: Discussionsupporting

confidence: 83%

“…But despite high doses of pancreatic enzymes used during therapy, normalization of digestion does not often occur and only partial corrections of the malnutrition have been reported [ 6 ]. Recent studies on an EPI pig model have shown a strong deteriorative effect of EPI on brain function and morphology [ 7 ]. However, it was also demonstrated that enrichment of the diet with pancreatic-like enzymes of microbial origin (PLEM) restored morpho-functional brain parameters [ 7 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies on an EPI pig model have shown a strong deteriorative effect of EPI on brain function and morphology [ 7 ]. However, it was also demonstrated that enrichment of the diet with pancreatic-like enzymes of microbial origin (PLEM) restored morpho-functional brain parameters [ 7 – 9 ]. Treatment of the malabsorption and gastrointestinal (GI) symptoms in EPI patients using the standard of porcine enzyme replacement therapy (PERT) is highly patient-dependent and only partially corrects the symptoms [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Pancreatic-like enzymes of microbial origin restore growth and normalize lipid absorption in a pig model with exocrine pancreatic insufficiency

Pierzynowska¹,

Piedra²,

Szymańczyk³

et al. 2018

aoms

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IntroductionThe standard therapy for exocrine pancreatic insufficiency (EPI) is porcine-derived pancreatic enzyme replacement therapy (PERT). In the present study we tested a new approach with a mixture of pancreatic-like enzymes of microbial origin (PLEM) in a 1-week efficacy study in EPI pigs. In addition to the conventionally used coefficient of fat and nitrogen absorption (CFA and CNA), parameters that more accurately reflect the nutritional and health status, such as changes in the lipemic index (LI), plasma triglyceride (TG) and non-esterified fatty acid (NEFA) levels, and somatic growth, were determined.Material and methodsA PLEM dose containing 120 000 active lipase units, 80 000 active protease units and 12 000 active amylase units (all from Sigma, St. Louis, MO) was given as a powder, twice daily with a meal (40 g fat/meal) to 8 EPI pigs for 7 days. Ten healthy pigs were used as a comparator.ResultsThe PLEM enhanced fat and protein digestion, and reversed growth impairment in EPI pigs. With treatment, CFA and CNA increased by 59% and 43% (p < 0.05), respectively. Although fat and protein absorption were lower than in the comparator, the postprandial blood lipid profile was normal as in healthy pigs. The mucosal thickness significantly increased by 27%, 50% and 26%, in the proximal, middle, and distal jejunum (p < 0.05) with treatment and resembled that of healthy animals.ConclusionsPancreatic-like enzymes of microbial origin supported somatic growth and normalized the postprandial lipid profile. As a measure of efficacy, postprandial LI, TG and NEFA are viable endpoints to be explored in human trials.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pancreatic-like enzymes of microbial origin restore growth and normalize lipid absorption in a pig model with exocrine pancreatic insufficiency

Pierzynowska¹,

Piedra²,

Szymańczyk³

et al. 2018

aoms

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“…Tissues were homogenized and processed as previously described [ 11 ]. The concentrations of glial fibrillary acidic protein (GFAP) and neural cell adhesion molecule (NCAM) in the fractions were determined using ELISA as previously described [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Difference in Performance of EPI Pigs Fed Either Lipase-Predigested or Creon®-Supplemented Semielemental Diet

Pierzynowski

Socha-Banasiak

Sobol

et al. 2021

BioMed Research International

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Pancreatic enzyme replacement therapy (PERT) and fat predigestion are key in ensuring the optimal growth of patients with cystic fibrosis. Our study attempted to highlight differences between fat predigestion and conventional PERT on body composition of young pigs with exocrine pancreatic insufficiency (EPI). EPI and healthy pigs were fed with high-fat diet for six weeks. During the last two weeks of the study, all pigs received additional nocturnal alimentation with Peptamen AF (PAF) and were divided into three groups: H—healthy pigs receiving PAF; P—EPI pigs receiving PAF+PERT; and L—EPI pigs receiving PAF predigested with an immobilized microbial lipase. Additional nocturnal alimentation increased the body weight gain of EPI pigs with better efficacy in P pigs. Humerus length and area in pigs in groups L and P were lower than that observed in pigs in group H ( p value 0.005-0.088). However, bone mineral density and strength were significantly higher in P and L as compared to that of H pigs ( p value 0.0026-0.0739). The gut structure was improved in P pigs. The levels of neurospecific proteins measured in the brain were mainly affected in P and less in L pigs as compared to H pigs. The beneficial effects of the nocturnal feeding with the semielemental diet in the prevention of EPI pigs’ growth/development retardation are differently modified by PERT or fat predigestion in terms of growth, bone properties, neurospecific protein distribution, and gut structure.

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Diet supplemented with pancreatic-like enzymes of microbial origin restores the hippocampal neuronal plasticity and behaviour in young pigs with experimental exocrine pancreatic insufficiency

Cited by 2 publications

References 28 publications

Pancreatic-like enzymes of microbial origin restore growth and normalize lipid absorption in a pig model with exocrine pancreatic insufficiency

Pancreatic-like enzymes of microbial origin restore growth and normalize lipid absorption in a pig model with exocrine pancreatic insufficiency

Difference in Performance of EPI Pigs Fed Either Lipase-Predigested or Creon®-Supplemented Semielemental Diet

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