Diet shapes the gut microbiota of the omnivorous cockroach Blattella germanica

Pérez‐Cobas, Ana Elena; Maiques, Elisa; Angelova, Alexandra; Carrasco, Purificación; Moya, Andrés; Latorre, Amparo

doi:10.1093/femsec/fiv022

Cited by 110 publications

(150 citation statements)

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“…The predominant archaeal taxon was Methanomicrococcus blatticola, a methanogen associated most commonly with cockroaches (34). These results agree well with those from previously published studies of cockroaches (17)(18)(19) all, most identified microbes were typical of those found in the guts of omnivores, including the human gut (13,35). However, many of the microbes found were unclassifiable above the class or family level, suggesting that they may belong to poorly characterized, insect-specific lineages.…”

Section: Resultssupporting

confidence: 89%

mentioning

confidence: 99%

“…As a result, we sought to determine the extent to which the response of the cockroach gut microbiota to dietary shifts resembles those identified in mammals. Several studies were conducted that examined the effect of diet in various cockroach species (15)(16)(17)(18)(19). These studies found a variety of results, with Schauer et al (18) reporting a highly stable core microbiome in Shelfordella lateralis, Bertino-Grimaldi et al (17) reporting a small but significant response to dietary shifts in P. americana, and Pérez-Cobas et al (19) reporting a strong response in Blattella germanica.…”

mentioning

confidence: 99%

“…Several studies were conducted that examined the effect of diet in various cockroach species (15)(16)(17)(18)(19). These studies found a variety of results, with Schauer et al (18) reporting a highly stable core microbiome in Shelfordella lateralis, Bertino-Grimaldi et al (17) reporting a small but significant response to dietary shifts in P. americana, and Pérez-Cobas et al (19) reporting a strong response in Blattella germanica. However, these studies typically focused on responses to a limited range of substrates, particularly lignocellulosic materials, and all but that reported by Pérez-Cobas (19) (three replicate experiments per treatment) lack replication or characterization of the interindividual variability in microbiome composition.…”

mentioning

confidence: 99%

“…These studies found a variety of results, with Schauer et al (18) reporting a highly stable core microbiome in Shelfordella lateralis, Bertino-Grimaldi et al (17) reporting a small but significant response to dietary shifts in P. americana, and Pérez-Cobas et al (19) reporting a strong response in Blattella germanica. However, these studies typically focused on responses to a limited range of substrates, particularly lignocellulosic materials, and all but that reported by Pérez-Cobas (19) (three replicate experiments per treatment) lack replication or characterization of the interindividual variability in microbiome composition. In this study, we utilized high-throughput 16S rRNA gene sequencing to characterize the hindgut microbiome of P. americana and its response to a wide range of dietary compositions, including high-fat, high-carbohydrate, and highprotein diets.…”

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confidence: 99%

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The Core Gut Microbiome of the American Cockroach, Periplaneta americana, Is Stable and Resilient to Dietary Shifts

Tinker

Ottesen

2016

Appl Environ Microbiol

106

131

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The omnivorous cockroach Periplaneta americana hosts a diverse hindgut microbiota encompassing hundreds of microbial species. In this study, we used 16S rRNA gene sequencing to examine the effect of diet on the composition of the P. americana hindgut microbial community. Results show that the hindgut microbiota of P. americana exhibit a highly stable core microbial community with low variance in compositions between individuals and minimal community change in response to dietary shifts. This core hindgut microbiome is shared between laboratory-hosted and wild-caught individuals, although wild-caught specimens exhibited a higher diversity of low-abundance microbes that were lost following extended cultivation under laboratory conditions. This taxonomic stability strongly contrasts with observations of the gut microbiota of mammals, which have been shown to be highly responsive to dietary change. A comparison of P. americana hindgut samples with human fecal samples indicated that the cockroach hindgut community exhibited higher alpha diversity but a substantially lower beta diversity than the human gut microbiome. This suggests that cockroaches have evolved unique mechanisms for establishing and maintaining a diverse and stable core microbiome. IMPORTANCEThe gut microbiome plays an important role in the overall health of its host. A healthy gut microbiota typically assists with defense against pathogens and the digestion and absorption of nutrients from food, while dysbiosis of the gut microbiota has been associated with reduced health. In this study, we examined the composition and stability of the gut microbiota from the omnivorous cockroach Periplaneta americana. We found that P. americana hosts a diverse core gut microbiome that remains stable after drastic long-term changes in diet. While other insects, notably ant and bee species, have evolved mechanisms for maintaining a stable association with specific gut microbiota, these insects typically host low-diversity gut microbiomes and consume specialized diets. In contrast, P. americana hosts a gut microbiota that is highly species rich and consumes a diverse solid diet, suggesting that cockroaches have evolved unique mechanisms for developing and maintaining a stable gut microbiota. Most insects host simple gut microbial communities, with only a few unique species represented; the reed beetle, honey bee, fruit fly, and gypsy moth all have fewer than 10 species of bacteria in their guts (1). The low complexity of these communities has been attributed to selective pressures dictated by host physiology (2) and the lack of extensive parental contact with offspring in many insects, which offers few opportunities for vertical and social transmission of gut microbes (1, 3). However, certain social and/or gregarious insect species, including cockroaches and their close relatives, the termites, host complex gut communities comprising hundreds of species (1,4,5).The cockroach gut is composed of three compartments: the foregut, midgut, and hindgut. Of the three, ...

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Section: Resultssupporting

confidence: 89%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The Core Gut Microbiome of the American Cockroach, Periplaneta americana, Is Stable and Resilient to Dietary Shifts

Tinker

Ottesen

2016

Appl Environ Microbiol

106

131

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Similar gut bacteria composition in Apriona germari on two preferred host plants

Zhang

Wang

et al. 2022

Arch Insect Biochem Physiol

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Apriona germari is one of the most serious wood‐boring pests that cause damage to economic and landscaping trees and has adapted to a wide range of plants as diet. Gut bacteria play an important role in biology and ecology of herbivores, especially in growth and adaptation. To investigate how plant hosts shape A. germari gut microbiota, A. germari larvae were collected from Populus tomentosa and Malus pumilal, and gut microbiomes were sequenced based on 16S rDNA high‐throughput sequencing technology. A total of 853,424 high‐quality reads were obtained and clustered into 196 operational taxonomic units under a 97% similarity cutoff, which were annotated into 8 phyla, 10 classes, 21 orders, 34 families, 59 genera, and 39 species. Gibbsiella was the most dominant genus of intestinal bacteria, followed by Enterobacter and Acinetobacter. No significant difference was observed in larvae gut bacterial richness and diversity of A. germari collected from two hosts, though alpha diversity showed that the richness of gut bacteria in A. germari larvae collected on P. tomentosa was slightly higher than that in A. germari on M. pumilal, and beta diversity showed little difference between two host plants. The functional abundance analysis of the detected bacteria revealed fermentation, chemoheterotrophy, symbionts, and nitrate relative functions that highly possibly support wood‐boring beetles to feed on woody tissues. Our study provided a theoretical basis for investigating the function of intestinal symbiosis bacteria of A. germari.

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The influence of diet and environment on the gut microbial community of field crickets

Stat

Bunce

et al. 2018

Ecology and Evolution

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The extent to which diet and environment influence gut community membership (presence or absence of taxa) and structure (individual taxon abundance) is the subject of growing interest in microbiome research. Here, we examined the gut bacterial communities of three cricket groups: (1) wild caught field crickets, (2) laboratory‐reared crickets fed cat chow, and (3) laboratory‐reared crickets fed chemically defined diets. We found that both environment and diet greatly altered the structure of the gut bacterial community. Wild crickets had greater gut microbial diversity and higher Firmicutes to Bacteroidetes ratios, in contrast to laboratory‐reared crickets. Predictive metagenomes revealed that laboratory‐reared crickets were significantly enriched in amino acid degradation pathways, while wild crickets had a higher relative abundance of peptidases that would aid in amino acid release. Although wild and laboratory animals differ greatly in their bacterial communities, we show that the community proportional membership remains stable from Phylum to Family taxonomic levels regardless of differences in environment and diet, suggesting that endogenous factors, such as host genetics, have greater control in shaping gut community membership.

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Diet shapes the gut microbiota of the omnivorous cockroach Blattella germanica

Cited by 110 publications

References 53 publications

The Core Gut Microbiome of the American Cockroach, Periplaneta americana, Is Stable and Resilient to Dietary Shifts

The Core Gut Microbiome of the American Cockroach, Periplaneta americana, Is Stable and Resilient to Dietary Shifts

Similar gut bacteria composition in Apriona germari on two preferred host plants

The influence of diet and environment on the gut microbial community of field crickets

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