Diet influences the intake target and mitochondrial functions of Drosophila melanogaster males

Pichaud, Nicolas; Messmer, Marie C.; Correa, Carolina; Ballard, J. William O.

doi:10.1016/j.mito.2013.05.008

Cited by 41 publications

(52 citation statements)

References 30 publications

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“…Importantly, Pichaud et al. () demonstrated that genetically based effects occurred only when dietary restrictions were in place, that is, the conditions of seasonally low food abundances were mimicked. The work by Moreno‐Loshuertos et al.…”

Section: Discussion and Interpretationmentioning

confidence: 99%

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Review and meta-analysis of natural selection in mitochondrial complex I in metazoans

Garvin

Bielawski

Sazanov

et al. 2014

J Zoolog Syst Evol Res

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Variation in mitochondrial DNA is often assumed to be neutral and is used to construct the genealogical relationships among populations and species. However, if extant variation is the result of episodes of positive selection, these genealogies may be incorrect, although this information itself may provide biologically and evolutionary meaningful information. In fact, positive Darwinian selection has been detected in the mitochondrial‐encoded subunits that comprise complex I from diverse taxa with seemingly dissimilar bioenergetic life histories, but the functional implications of the selected sites are unknown. Complex I produces roughly 40% of the proton flux that is used to synthesize ATP from ADP, and a functional model based on the high‐resolution structure of complex I described a unique biomechanical apparatus for proton translocation. We reported positive selection at sites in this apparatus during the evolution of Pacific salmon, and it appeared this was also the case in published reports from other taxa, but a comparison among studies was difficult because different statistical tests were used to detect selection and oftentimes, specific sites were not reported. Here we review the literature of positive selection in mitochondrial genomes, the statistical tests used to detect selection, and the structural and functional models that are currently available to study the physiological implications of selection. We then search for signatures of positive selection among the coding mitochondrial genomes of 237 species with a common set of tests and verify that the ND5 subunit of complex I is a repeated target of positive Darwinian selection in diverse taxa. We propose a novel hypothesis to explain the results based on their bioenergetic life histories and provide a guide for laboratory and field studies to test this hypothesis.

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Section: Discussion and Interpretationmentioning

confidence: 99%

“…Most recently, they have demonstrated that the complex I activity of the ND2‐specific haplotype is reduced depending on dietary intake (Pichaud et al. ).…”

Section: Reviewmentioning

confidence: 99%

Review and meta-analysis of natural selection in mitochondrial complex I in metazoans

Garvin

Bielawski

Sazanov

et al. 2014

J Zoolog Syst Evol Res

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“…and are presented with the abbreviation(s) of the complex(es) contributing to the electron flux, followed by the state of respiration (complex-STATE, see Pichaud et al, 2013). All substrate concentrations were optimized to stimulate maximum rates of mitochondrial oxygen consumption.…”

Section: Substrate-uncoupler-inhibitor Titration (Suit) Protocol For mentioning

confidence: 99%

Dynamic changes in cardiac mitochondrial metabolism during warm acclimation in rainbow trout

Pichaud

Ekström

Hellgren

et al. 2017

Journal of Experimental Biology

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Although the mitochondrial metabolism responses to warm acclimation have been widely studied in fish, the time course of this process is less understood. Here, we characterized the changes of rainbow trout (Oncorhynchus mykiss) cardiac mitochondrial metabolism during acute warming from 10 to 16°C, and during the subsequent warm acclimation for 39 days. We repeatedly measured mitochondrial oxygen consumption in cardiac permeabilized fibers and the functional integrity of mitochondria (i.e. mitochondrial coupling and cytochrome c effect) at two assay temperatures (10 and 16°C), as well as the activities of citrate synthase (CS) and lactate dehydrogenase (LDH) at room temperature. LDH and CS activities significantly increased between day 0 (10°C acclimated fish) and day 1 (acute warming to 16°C) while mitochondrial oxygen consumption measured at respective in vivo temperatures did not change. Enzymatic activities and mitochondrial oxygen consumption rates significantly decreased by day 2, and remained stable during warm acclimation (days 2-39). The decrease in rates of oxygen between day 0 and day 1 coincided with an increased cytochrome c effect and a decreased mitochondrial coupling, suggesting a structural/ functional impairment of mitochondria during acute warming. We suggest that after 2 days of warm acclimation, a new homeostasis is reached, which may involve the removal of dysfunctional mitochondria. Interestingly, from day 2 onwards, there was a lack of differences in mitochondrial oxygen consumption rates between the assay temperatures, suggesting that warm acclimation reduces the acute thermal sensitivity of mitochondria. This study provides significant knowledge on the thermal sensitivity of cardiac mitochondria that is essential to delineate the contribution of cellular processes to warm acclimation.

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“…Pichaud et al . () observed that a small set of known mutational differences influence OXPHOS complex I activity when adult flies are fed with the more stressful 1 : 12 P : C diet, but not when they are fed the 1 : 3 diet that is more common in most laboratories. Certainly, the availability of nutritional resources will vary geographically and seasonally.…”

Section: The Genotype By Environment Effectmentioning

confidence: 99%

Mitochondrial DNA: more than an evolutionary bystander

2013

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Summary1. The vast majority of studies employing mtDNA in evolutionary biology and ecology have used it as a means to infer demographic and historical patterns without pondering the underlying functional implications. In contrast, the biochemical and medical communities often aim to understand the influence of specific mtDNA mutations on mitochondrial functions, but rarely consider the evolutionary and ecological implications. 2. Ongoing research has shown that mtDNA mutations can profoundly affect mitochondrial function in humans and other animals. If the mutation (or set of mutations) is pathogenic, mitochondrial malfunction may be detected from early age. In nature, however, most mutations are not highly deleterious and may exist at intermediate frequency in populations. 3. In this review, we suggest that knowledge of the underlying biochemistry and functions of mitochondria can facilitate a more complete determination of the evolutionary dynamics of mtDNA and its influence on the life-history traits of organisms. With this approach, it is possible to use biochemistry to link the genotype with the phenotype. 4. After reviewing the literature, we conclude that there can be physiological and evolutionary trade-offs in the way that mitochondrial mutations can affect age classes and/or fitness components and that these effects may depend on the environment. Through these trade-offs, it may be possible for specific mtDNA mutations to have unequal fitness in different nuclear genetic backgrounds and also in different environments.

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Diet influences the intake target and mitochondrial functions of Drosophila melanogaster males

Cited by 41 publications

References 30 publications

Review and meta-analysis of natural selection in mitochondrial complex I in metazoans

Review and meta-analysis of natural selection in mitochondrial complex I in metazoans

Dynamic changes in cardiac mitochondrial metabolism during warm acclimation in rainbow trout

Mitochondrial DNA: more than an evolutionary bystander

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