Diet-induced obesity impairs endometrial stromal cell decidualization: a potential role for impaired autophagy

Rhee, Jongeun; Saben, Jessica; Mayer, Allyson L.; Schulte, M.; Asghar, Zeenat; Stephens, Claire; M.‐Y., Maggie; Moley, Kelle H.

doi:10.1093/humrep/dew048

Cited by 123 publications

(92 citation statements)

References 47 publications

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“…Atg7 is required for acrosome biogenesis during spermatogenesis, and germ cell-specific Atg7 -knockout mice display a phenotype similar to human globozoospermia19. It was also reported that the activation of autophagy is indispensable for the process of decidualization, which was confirmed in diet-induced obesity female mice68. Consistent with these facts, we also found that CQ impaired the viability and motility of sperm in vitro and in vivo at baseline, implying that autophagy is also indispensable for the physiological process of spermatogenesis.…”

Section: Discussionmentioning

confidence: 85%

Diet-induced obesity impairs spermatogenesis: a potential role for autophagy

Yan

Yin

et al. 2017

Sci Rep

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Autophagy is an evolutionarily conserved process that plays a crucial role in maintaining a series of cellular functions. It has been found that autophagy is closely involved in the physiological process of spermatogenesis and the regulation of sperm survival and motility. However, the role of autophagy in high-fat diet (HFD)-induced impaired spermatogenesis remains unknown. This study was designed to investigate the role of autophagy in HFD-induced spermatogenesis deficiency and employed chloroquine (CQ) to inhibit autophagy and rapamycin (RAP) to induce autophagy. 3-methyladenine (3-MA) and CQ were administered via intratesticular injection in vivo. The effects of CQ and 3-MA on the parameters of spermatozoa co-cultured with palmitic acid (PA) in vitro were also investigated. Human semen samples from obese, subfertile male patients were also collected to examine the level of autophagy. The results suggested that HFD mice subjected to CQ showed improved spermatogenesis. Inhibiting autophagy with CQ improved the decreased fertility of HFD male mice. Moreover, the in vivo and in vitro results indicated that both CQ and 3-MA could suppress the pathological changes in spermatozoa caused by HFD or PA treatment. Additionally, the excessive activation of autophagy was also observed in sperm samples from obese, subfertile male patients.

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Section: Discussionmentioning

confidence: 85%

Diet-induced obesity impairs spermatogenesis: a potential role for autophagy

Yan

Yin

et al. 2017

Sci Rep

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“…However, we only collected data for mice on pregnant D4. A study carried by Julie S. Rhee reported that a decreased autophagy was observed in a high-fat/highsugar diet-exposed mice decidualization [43]. More research on other pregnant days as well as the decidualization is needed.…”

Section: Discussionmentioning

confidence: 99%

High insulin impaired ovarian function in early pregnant mice and the role of autophagy in this process

et al. 2017

Endocr J

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METABOLIC DISORDERS, such as PCOS (polycystic ovary syndrome) and T2DM (type 2 diabetes mellitus), are reported to be associated with high miscarriage rates, decreased pregnancy rates, and low live birth rates [1]. PCOS and T2DM are characterized by hyperinsulinemia [2]. PCOS is an endocrine and metabolic disorder with a prevalence ranging from 5 to 13% in women of reproductive age. Moreover, PCOS is often accompanied by menstrual dysfunction, anovulaHigh insulin impaired ovarian function in early pregnant mice and the role of autophagy in this process Abstract. Metabolic disorders, such as PCOS (polycystic ovarian syndrome) and T2DM (type 2 diabetes mellitus), are associated with menstrual dysfunction, anovulation, infertility, and early pregnancy loss. Ovarian dysfunction is not only related to low pregnancy rates but also to the increased risk of miscarriage. Women with PCOS or T2DM, characterized by hyperinsulinemia, commonly experience ovarian dysfunction. In this study, we first explored whether high insulin levels directly affected ovarian functioning during embryo implantation. Mice in the insulin-treated group were given a subcutaneous injection of human recombinant insulin. After insulin treatment, serum levels of E2 (estrogen), PROG (progesterone), LH (luteinizing hormone), and FSH (follicle-stimulating hormone) were obviously lower, and there was a significant decrement of ovarian GDF9 (growth differentiation factor 9) mRNA. H&E (hematoxylin and eosin) staining showed a greater number of immature follicles and less luteinization in the insulin group. Further autophagy was studied in this process. A significant increase of P62 (SQSTM1/Sequestosome1) and a decrease of Cathepsin B, BECN1 (Beclin 1), and ULK1 (Unc-51-like kinase 1) mRNA in ovary was found in the insulin group. Western blot analysis showed that the expressions of LC3 (microtubule-associated protein 1 light chain 3), BECN1, and Cathepsin B proteins in ovaries from insulin group were obviously reduced, while P62 proteins were significantly increased. All these results illustrated that insulin could directly impair ovarian function during embryo implantation and the imbalance of ovarian autophagy due to insulin. Autophagy could enhance the impaired ovarian function results from insulin.

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“…Class 3 semaphorins (Sema3C) secrete guidance proteins [24] and Fasn, which are involved in the adipose tissue transcriptional response of lipid metabolism [25], were upregulated. Ugt2b, UDP glycosyltransferase 2 family, polypeptide B [26], and Igfbp1 (insulin-like growth factor binding protein 1) [27] were down-regulated. UDP glycosyltransferase (UGT), mainly UDP glucuronosyltransferases, its subfamily UGT 2B enzymes related to xenobiotic-metabolizing enzymes and proteoglycan synthesis [28], and recapitulated dysregulation patterns of major UDP-glucuronosyltransferases (UGTs) were induced by HFD [29].…”

Section: Discussionmentioning

confidence: 99%

Anti-Obesity Effect of Bombus ignitus Queen Glycosaminoglycans in Rats on a High-Fat Diet

Ahn

Kim

et al. 2017

IJMS

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The mechanism of functional insect glycosaminoglycan (GAG) on obesity caused a high fat diet has not yet been elucidated. Therefore, insect glycosaminoglycans derived from Isaria sinclairii, Bombus ignitus (a type of bumblebee) queen, and Gryllus bimaculatus were purified and investigated as a potential functional food. 14-week old male Wistar rats were fed a high-fat diet (HFD) for 6 weeks. There were five groups that received daily intraperitoneal administration of phosphate buffered saline (PBS, control), GbG (GAG from Gryllus bimaculatus) 10 mg/kg, ISG (GAG from Isaria sinclairii) 10 mg/kg, IQG (GAG from Bombus ignites) 10 mg/kg, or Pravastatin (2 mg/kg). All treatments were performed for one month. IQG produced a potential anti-inflammatory effect with the inhibition of c-reactive protein and sero-biochemical parameters of phospholipids and free fatty acids indicative of an anti-hyperlipidemic effect. Abdominal and epididymidal fat weight were reduced in conjunction with a mild increase in body weight. The level of laminin in HMVEC-C cells or fibronectin in HFD rat hepatocytes was significantly affected by these GAG treatments, which regulated adipogenesis and adipocyte function. Compared to the control rats, IQG-treated rats displayed up-regulation of 87 genes (test:control ratio >2.0) including fatty acid synthase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase, with the down-regulation of 47 genes including the uridine diphosphate (UDP) glycosyltransferase 2 families, polypeptidase B, and insulin-like growth factor binding protein 1. The data suggest that IQG could potentially prevent or treat fatty liver or hyperlipidemia.

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Diet-induced obesity impairs endometrial stromal cell decidualization: a potential role for impaired autophagy

Abstract: Grants include NIH 5T32HD040135-12 (J.S.R.), R01 HD065435 (K.H.M.), NIH T32 HD049305 (J.L.S.) and ACOG Research Grant (M.B.S.). The authors report no conflicts of interest.

Cited by 123 publications

References 47 publications

Diet-induced obesity impairs spermatogenesis: a potential role for autophagy

Diet-induced obesity impairs spermatogenesis: a potential role for autophagy

High insulin impaired ovarian function in early pregnant mice and the role of autophagy in this process

Anti-Obesity Effect of Bombus ignitus Queen Glycosaminoglycans in Rats on a High-Fat Diet

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