Diet, habitat environment and lifestyle conversion affect the gut microbiomes of giant pandas

Jin, Lan; Huang, Yan; Yang, Sheng; Wu, Daifu; Li, Caiwu; Deng, Wenwen; Zhao, Ke; He, Yongguo; Li, Bei; Zhang, Guiquan; Xiong, Yaowu; Wei, Rongping; Guo, Li; Wu, Hongning; Zhang, Hemin; Zou, Likou

doi:10.1016/j.scitotenv.2021.145316

Cited by 34 publications

(43 citation statements)

References 97 publications

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“…Although, the overall diversity of gut microbes was unrelated to diet, we found that changes in diet had influences on specific, related microbiota. In human studies, it has been confirmed that dietary fat consumption is positively correlated with abundant Actinobacteria and negatively correlated with abundant Firmicutes (Abecia et al, 2007;Wu et al, 2011;Jin et al, 2021). Compared with non-lactating bats, the lactating bats had a higher relative abundance of Actinobacteria and lower relative abundance of Firmicutes, which would be useful for consuming more fat and reducing fat storage, thus improving the survival rate of offspring (Tarnaud, 2006;Hamady et al, 2008).…”

Section: The Gut Microbiota Changesmentioning

confidence: 95%

Differences in Diet and Gut Microbiota Between Lactating and Non-lactating Asian Particolored Bats (Vespertilio sinensis): Implication for a Connection Between Diet and Gut Microbiota

Chu

Yao

et al. 2021

Front. Microbiol.

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In mammals, lactation is considered the most energetically costly phase for females. To meet nutritional and energy demands, lactating females usually change feeding patterns by eating food that is higher in protein and calories. Their gut microbes respond accordingly to help adapt to the changes in diet. In this study, we examined differences in diet and gut microbial composition between lactating and non-lactating Asian particolored bats (Vespertilio sinensis) using COI and 16S amplicon sequencing. When compared with non-lactating bats, we found that the diversity and composition of lactating bats’ diets differed; the proportion of Diptera increased and Coleoptera and Orthoptera decreased significantly. This could be attributed to the easy availability and high protein content of Diptera. Comparative analysis of the gut microbiota of lactating and non-lactating females showed that although the diversity of gut microbiota did not change, the relative abundance of specific gut microbiota associated with a particular diet did change. For example, when the consumption of Coleoptera decreased in lactating bats, the relative abundance of Lactobacillaceae was also reduced. Lactobacillaceae are thought to be involved in the digestion of Coleopteran exoskeletons. This study suggests that during lactation, Asian particolored bats eat a diet that yields higher levels of protein, and at the same time, the abundance of specific gut microbes change to help their hosts adapt to these changes in diet.

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Section: The Gut Microbiota Changesmentioning

confidence: 95%

Differences in Diet and Gut Microbiota Between Lactating and Non-lactating Asian Particolored Bats (Vespertilio sinensis): Implication for a Connection Between Diet and Gut Microbiota

Chu

Yao

et al. 2021

Front. Microbiol.

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“…Second, adult individuals tend to have more complex diets and living conditions than juveniles, resulting in changes in intestinal microbial diversity. Jin et al (2021) evaluated the effects of dietary changes, habitat changes, and lifestyle shifts on the gut microbiota of giant pandas with high-throughput sequencing and genome-resolved metagenomics. The results showed that high-fiber diets significantly increased the species diversity and decreased the richness of gut bacterial communities ( Jin et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“… Jin et al (2021) evaluated the effects of dietary changes, habitat changes, and lifestyle shifts on the gut microbiota of giant pandas with high-throughput sequencing and genome-resolved metagenomics. The results showed that high-fiber diets significantly increased the species diversity and decreased the richness of gut bacterial communities ( Jin et al, 2021 ). Third, the habitat can also greatly affect bacterial communities.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Intestinal Microflora Between Two Developmental Stages of Rimicaris kairei, a Hydrothermal Shrimp From the Central Indian Ridge

Lian

Zhu

et al. 2022

Front. Microbiol.

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Despite extreme physical and chemical characteristics, deep-sea hydrothermal vents provide a place for fauna survival and reproduction. The symbiotic relationship of chemotrophic microorganisms has been investigated in the gill of Rimicaris exoculata, which are endemic to the hydrothermal vents of the Mid-Atlantic Ridge. However, only a few studies have examined intestinal symbiosis. Here, we studied the intestinal fauna in juvenile and adult Rimicaris kairei, another species in the Rimicaris genus that was originally discovered at the Kairei and Edmond hydrothermal vent fields in the Central Indian Ridge. The results showed that there were significant differences between juvenile and adult gut microbiota in terms of species richness, diversity, and evenness. The values of Chao1, observed species, and ASV rarefaction curves indicated almost four times the number of species in adults compared to juveniles. In juveniles, the most abundant phylum was Deferribacterota, at 80%, while in adults, Campilobacterota was the most abundant, at 49%. Beta diversity showed that the intestinal communities of juveniles and adults were clearly classified into two clusters based on the evaluations of Bray–Curtis and weighted UniFrac distance matrices. Deferribacteraceae and Sulfurovum were the main featured bacteria contributing to the difference. Moreover, functional prediction for all of the intestinal microbiota showed that the pathways related to ansamycin synthesis, branched-chain amino acid biosynthesis, lipid metabolism, and cell motility appeared highly abundant in juveniles. However, for adults, the most abundant pathways were those of sulfur transfer, carbohydrate, and biotin metabolism. Taken together, these results indicated large differences in intestinal microbial composition and potential functions between juvenile and adult vent shrimp (R. kairei), which may be related to their physiological needs at different stages of development.

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“…However, plant protein has certain disadvantages relative to FM as a source of feed protein (Jannathulla et al, 2019). For example, the high content of cellulose and imbalance of amino acids in feed might cause damage to the intestine, thereby destroying the balance of intestinal microbiota (Jin et al, 2021). In this research, we found that when the replacement level of LCSM exceeded 20%, intestinal permeability increased, immune dysfunction and microbiota dysbiosis occurred in PI, MI and DI of juvenile golden pompano.…”

Section: Discussionmentioning

confidence: 99%

Effects of fish meal replacement with low‐gossypol cottonseed meal on the intestinal barrier of juvenile golden pompano ( Trachinotus ovatus )

Qian

Liu

et al. 2021

Aquaculture Research

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The effects of replacing fish meal (FM) with low-gossypol cottonseed meal (LCSM) on the intestinal barrier in different segments (PI, proximal intestine; MI, middle intestine; DI, distal intestine) were studied in juvenile golden pompano (Trachinotus ovatus) using 6-week feeding trials. FM of 0% (diet_1), 20% (diet_2), 40% (diet_3) and 60% (diet_4) was replaced by LCSM. The FM-based diet (diet_1) containing 25% FM was used as control group. The intestinal morphology, mRNA expression of tight junctionrelated genes (ZO-1, Occludin, Claudin-12), antioxidant enzyme-related genes (CAT, GPx, GR, CuZnSOD) and non-specific immune response-related genes (IL-8, TNFα, C4) and microbiota of different intestine segments were detected. The results showed that the number and length of microvilli and intestinal wall thickness decreased significantly in PI of diet_3 and diet_4 groups. Meanwhile, mRNA expression of Occludin, Clauding-12 and C4 was up-regulated in PI and DI of diet_3 group; the expression of GR and CuZnSOD was up-regulated in PI and MI with increasing LCSM replacement; the expression of TNFα was up-regulated in the three segments of diet_3 group. Further, 16S rRNA amplicon sequencing revealed a significant reduction in the richness and diversity of intestinal microbial in diet_3 and diet_4 groups. At the family level and genera level, the abundance of Nocardiaceae and Rhodococcus was up-regulated in the three segments of diet_3 and diet_4 groups respectively. These results suggested that the replacement of LCSM by more than 20% causes the intestinal barrier function of juvenile golden pompano to be affected. These provide a reference for sustainable aquaculture.

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Diet, habitat environment and lifestyle conversion affect the gut microbiomes of giant pandas

Cited by 34 publications

References 97 publications

Differences in Diet and Gut Microbiota Between Lactating and Non-lactating Asian Particolored Bats (Vespertilio sinensis): Implication for a Connection Between Diet and Gut Microbiota

Differences in Diet and Gut Microbiota Between Lactating and Non-lactating Asian Particolored Bats (Vespertilio sinensis): Implication for a Connection Between Diet and Gut Microbiota

Comparative Analysis of Intestinal Microflora Between Two Developmental Stages of Rimicaris kairei, a Hydrothermal Shrimp From the Central Indian Ridge

Effects of fish meal replacement with low‐gossypol cottonseed meal on the intestinal barrier of juvenile golden pompano ( Trachinotus ovatus )

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