Diet-derived vicilins detected in eggs laid by a double-mated female Callosobruchus maculatus originate from nuptial gifts donated by both male partners

Silva, Carlos P.; Kunz, Daniele; Linhares, Ricardo T.; Samuels, Richard Ian; Macedo, Maria Lígia Rodrigues

doi:10.1016/j.jspr.2015.07.003

Cited by 5 publications

(1 citation statement)

References 22 publications

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“…Menurut Bawa et al (2017) serangga akan menempatkan telur-telurnya pada makanan yang sesuai untuk generasinya. Pada sisi lain, serangga memilih biji kacang karena aroma dan kandungan nutrisi dari masingmasing kacang sebagai stumulus (Silva et al, 2015). .…”

Section: Hasil Dan Pembahasanunclassified

Perubahan perilaku Callosobruchus maculatus Fabricius terhadap warna cahaya pada kacang-kacangan di penyimpanan

Hakim

Irhamni²

2019

J. AGRO

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Callosobruchus maculatus (Fab.) adalah salah satu species serangga dari ordo Coleoptera, family Brucidae yang merupakan hama kacang-kacangan di gudang penyimpanan. Kerusakan kacang selama penyimpanan diawali dengan perilaku oviposisi telur serangga betina dewasa pada kotiledon biji kacang. Serangga C. maculatus (Fab.) tidak menyukai tempat dengan cahaya terang. Penelitian ini bertujuan untuk mengamati perubahan perilaku oviposisi dan kopulasi serangga dewasa pada kacang-kacangan dengan penerangan empat warna cahaya pada ruang penyimpanan. Pengamatan dan analisis data menggunakan Rancangan Acak Lengkap Faktorial. Faktor petama menggunakan cahaya lampu merah, kuning, hijau dan putih, sedangkan faktor kedua terdiri dari tiga jenis kacang (Fabaceae) yaitu kacang hijau, kacang kedelai dan kacang merah. Hasil penelitian menunjukkan perilaku oviposisi telur terjadi pada cahaya lampu kuning, sedangkan perilaku kopulasi terjadi pada cahaya lampu merah. Cahaya lampu merah dan kuning dapat memengaruhi perilaku oviposisi dan kopulasi Callosobruchus maculatus (Fab.).ABSTRACTCallosobruchus maculatus (Fab.) is one of the insect species of the order Coleoptera, family Brucidae which is a pest for stored beans. Damage to beans during the storage starts with the behavior of the egg oviposition of adult female insects on bean seed cotyledons. C. maculatus (Fab.) do not like to be in a bright place. This study aimed to observe changes in the behavior of oviposition and copulation of adult insects in beans with four-colour lighting in the storage. The observation and analysis of data used factorial completely randomized design. The first factor were light colors; red, yellow, green, and white light while the second factor consists of three types of beans (Fabaceae); green beans, soybeans and red beans. The results showed that copulation behavior occurred in red light. While the oviposition behavior of eggs occured in yellow light. The red and yellow lights can affect the behavior of oviposistion and copulation of C.maculatus (Fab.).

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Section: Hasil Dan Pembahasanunclassified

Perubahan perilaku Callosobruchus maculatus Fabricius terhadap warna cahaya pada kacang-kacangan di penyimpanan

Hakim

Irhamni²

2019

J. AGRO

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Receptor mediated endocytosis of vicilin in Callosobruchus maculatus (Coleoptera: Chrysomelidae) larval midgut epithelial cells

Kunz

Oliveira

Uchôa

et al. 2017

Comparative Biochemistry and Physiology Part B: Biochemistry an

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The transport of proteins across the intestinal epithelium of insects is still not well understood. There is evidence that vicilin, a major storage protein of cowpea seeds (Vigna unguiculata), is internalized in larvae of the seed-beetle Callosobruchus maculatus. It has been reported that this vicilin interacts with proteins present in the microvillar membranes of columnar cells along the digestive tract of the larvae. In the present work, we studied the cellular pathway involved in endocytosis of vicilin in larval C. maculatus by employing ex vivo experiments. In the ex vivo approach, we incubated FITC-labelled vicilin with isolated midgut wholemounts in the absence or in the presence of endocytosis inhibitors. The fate of labelled or non-labelled globulins was monitored by confocal microscopy and fluorescence measurement. Our results suggest that the internalization of vicilins is due to receptor-mediated endocytosis. Here we report the identity of a microvillar vicilin-binding protein that was purified using affinity chromatography on a vicilin-sepharose column. The putative vicilin receptor showed high homology to proteins with the CRAL-TRIO domain, specifically the Sec14 superfamily member α-tocopherol transfer protein. The precise mechanism involved in vicilin internalization was defined through the use of specific inhibitors of the endocytosis pathway. The inhibitors filipin III and nystatin significantly inhibited the endocytosis of vicilin, while chlorpromazine and phenylarsine oxide had a much lower effect on endocytosis, suggesting that the endocytic pathway is predominantly mediated by caveolin.

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Phaseolin ingestion affects vesicular traffic causing oxidative stress in the midgut of Callosobruchus maculatus larvae

Kunz

Oliveira

Brascher

et al. 2019

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Diet-derived vicilins detected in eggs laid by a double-mated female Callosobruchus maculatus originate from nuptial gifts donated by both male partners

Cited by 5 publications

References 22 publications

Perubahan perilaku Callosobruchus maculatus Fabricius terhadap warna cahaya pada kacang-kacangan di penyimpanan

Perubahan perilaku Callosobruchus maculatus Fabricius terhadap warna cahaya pada kacang-kacangan di penyimpanan

Receptor mediated endocytosis of vicilin in Callosobruchus maculatus (Coleoptera: Chrysomelidae) larval midgut epithelial cells

Phaseolin ingestion affects vesicular traffic causing oxidative stress in the midgut of Callosobruchus maculatus larvae

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