Abstract:Tomato (Solanum lycopersicum L.) is one of the most important vegetables in the world. Its extensive cultivation has made this plant the target of many viral, fungal, and bacterial diseases. Among them, the bacterial canker of tomato caused by Clavibacter michiganensis (Cm) has been named one of the most devastating diseases affecting the tomato industry worldwide. It can significantly reduce the yields and profitability of this crop. One of the big challenges we found when working with Cm and trying to charac… Show more
Bacterial canker caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is a worldwide bacterial disease affecting tomato plants. Very few control methods exist and their efficacy is limited. In recent years, plant extracts were studied for their potential as a safe and eco‐friendly alternative to the use of chemical pesticides to control plant diseases. Recent work performed by our group revealed the antibacterial activity of an ethanolic sugar maple autumn‐shed leaf (SMASL) extract against bacterial plant pathogens. To further investigate the antibacterial and prophylactic potential of SMASL against bacterial canker, assays were performed (a) to determine the polyphenol content and the in vitro antibacterial activity of sugar maple leaf extracts against Cmm, (b) to evaluate the potential of SMASL extracts as a seed treatment against Cmm and (c) as a foliar application to control bacterial canker development in greenhouse‐ and field‐grown tomato plants. Variations in polyphenol content and antibacterial activity of sugar maple leaf extracts were studied monthly for a period of 2 years. Although polyphenol contents varied significantly, minimum inhibitory concentrations were constant between 1.56 and 3.13 mg/mL and minimum bactericidal concentrations between 12.5 and 25 mg/mL. SMASL extract at 25 mg/mL completely eliminated the pathogen from tomato seeds without negatively impacting on germination. SMASL extract foliar spray applications using concentrations of 6.25 and 12.5 mg/mL significantly repressed disease development under greenhouse and field conditions, showing better efficacy than copper octanoate. The antibacterial activity of SMASL extracts against Cmm shows great potential to control Cmm and bacterial canker in tomato.
Bacterial canker caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is a worldwide bacterial disease affecting tomato plants. Very few control methods exist and their efficacy is limited. In recent years, plant extracts were studied for their potential as a safe and eco‐friendly alternative to the use of chemical pesticides to control plant diseases. Recent work performed by our group revealed the antibacterial activity of an ethanolic sugar maple autumn‐shed leaf (SMASL) extract against bacterial plant pathogens. To further investigate the antibacterial and prophylactic potential of SMASL against bacterial canker, assays were performed (a) to determine the polyphenol content and the in vitro antibacterial activity of sugar maple leaf extracts against Cmm, (b) to evaluate the potential of SMASL extracts as a seed treatment against Cmm and (c) as a foliar application to control bacterial canker development in greenhouse‐ and field‐grown tomato plants. Variations in polyphenol content and antibacterial activity of sugar maple leaf extracts were studied monthly for a period of 2 years. Although polyphenol contents varied significantly, minimum inhibitory concentrations were constant between 1.56 and 3.13 mg/mL and minimum bactericidal concentrations between 12.5 and 25 mg/mL. SMASL extract at 25 mg/mL completely eliminated the pathogen from tomato seeds without negatively impacting on germination. SMASL extract foliar spray applications using concentrations of 6.25 and 12.5 mg/mL significantly repressed disease development under greenhouse and field conditions, showing better efficacy than copper octanoate. The antibacterial activity of SMASL extracts against Cmm shows great potential to control Cmm and bacterial canker in tomato.
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