Diesel exhaust and house dust mite allergen lead to common changes in the airway methylome and hydroxymethylome

Zhang, Xue; Rothenberg, Marc E.; Weirauch, Matthew T.; Zhang, Xiang; Burleson, J D; Brandt, Eric B.; Ji, Hong

doi:10.1093/eep/dvy020

Cited by 41 publications

(34 citation statements)

References 84 publications

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“…37 Also, HDM induces the same epigenetic modifications as diesel exhaust in an ex vivo model of inflammation in human bronchial epithelial cells. 38 These studies suggest that HDM also induces IgE-mediated bronchial inflammation by altering the epigenetic patterns of cells involved in bronchial homeostasis.…”

Section: New Concepts Regarding the Mechanisms Of Action Of Allergensmentioning

confidence: 97%

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

Ansotegui¹,

Melioli²,

Canonica³

et al. 2020

World Allergy Organization Journal

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Precision allergy molecular diagnostic applications (PAMD@) is increasingly entering routine care. Currently, more than 130 allergenic molecules from more than 50 allergy sources are commercially available for in vitro specific immunoglobulin E (sIgE) testing. Since the last publication of this consensus document, a great deal of new information has become available regarding this topic, with over 100 publications in the last year alone. It thus seems quite reasonable to publish an update. It is imperative that clinicians and immunologists specifically trained in allergology keep abreast of the new and rapidly evolving evidence available for PAMD@. PAMD@ may initially appear complex to interpret; however, with increasing experience, the information gained provides relevant information for the allergist. This is especially true for food allergy, Hymenoptera allergy, and for the selection of allergen immunotherapy. Nevertheless, all sIgE tests, including PAMD@, should be evaluated within the framework of a patient's clinical history, because allergen sensitization does not necessarily imply clinical relevant allergies.

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Section: New Concepts Regarding the Mechanisms Of Action Of Allergensmentioning

confidence: 97%

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

Ansotegui¹,

Melioli²,

Canonica³

et al. 2020

World Allergy Organization Journal

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“…Targeted microarray-based approaches are thus still frequently used. DNA methylation subsumes two different chemical modifications with different functional consequences-5-methylcytosine and 5-hydroxy-methylcytosine (Branco et al 2011;Zhang et al 2018). Most published studies do, however, not discriminate between both modifications.…”

Section: Which Omics Layers To Use For Toxicological Research Questionsmentioning

confidence: 99%

Prospects and challenges of multi-omics data integration in toxicology

et al. 2020

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Exposure of cells or organisms to chemicals can trigger a series of effects at the regulatory pathway level, which involve changes of levels, interactions, and feedback loops of biomolecules of different types. A single-omics technique, e.g., transcriptomics, will detect biomolecules of one type and thus can only capture changes in a small subset of the biological cascade. Therefore, although applying single-omics analyses can lead to the identification of biomarkers for certain exposures, they cannot provide a systemic understanding of toxicity pathways or adverse outcome pathways. Integration of multiple omics data sets promises a substantial improvement in detecting this pathway response to a toxicant, by an increase of information as such and especially by a systemic understanding. Here, we report the findings of a thorough evaluation of the prospects and challenges of multi-omics data integration in toxicological research. We review the availability of such data, discuss options for experimental design, evaluate methods for integration and analysis of multi-omics data, discuss best practices, and identify knowledge gaps. Re-analyzing published data, we demonstrate that multi-omics data integration can considerably improve the confidence in detecting a pathway response. Finally, we argue that more data need to be generated from studies with a multi-omics-focused design, to define which omics layers contribute most to the identification of a pathway response to a toxicant.Archives of Toxicology (2020) 94:371-388Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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“…Furthermore, prenatal exposure to either diesel exhaust particulates (DEP) or polycyclic aromatic hydrocarbons (PAHs), which are DEP‐derived toxins, is associated with a greater risk of allergic sensitization, early childhood wheeze, and asthma . Moreover, co‐exposure to DEP and house dust mite (HDM) has been shown to exacerbate allergic sensitization and to induce key characteristics of a more severe asthma . However, the key components in DEPs that are responsible for the aggravation of allergic response and the related underlying molecular mechanisms have not been revealed.…”

Section: Introductionmentioning

confidence: 99%

Benzo(a)pyrene facilitates dermatophagoides group 1 (Der f 1)‐induced epithelial cytokine release through aryl hydrocarbon receptor in asthma

Wang

Liu

et al. 2019

Allergy

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Background Environmental pollutants, which coexist with allergens, have been associated with the exacerbation of asthma. However, the underlying molecular mechanisms remain elusive. We sought to determine whether benzo(a)pyrene (BaP) co‐exposure with dermatophagoides group 1 allergen (Der f 1) can potentiate Der f 1‐induced asthma and its underlying mechanisms. Methods The effect of BaP was investigated in Der f 1‐induced mouse model of asthma, including airway hyper‐responsiveness, allergic inflammation, and epithelial‐derived cytokines. The impact of BaP on Der f 1‐induced airway epithelial cell oxidative stress (ROS) and cytokine release was further analyzed. The role of aryl hydrocarbon receptor (AhR) signaling in BaP‐promoted Der f 1‐induced ROS, cytokine production, and allergic inflammation was also investigated. Results Compared with Der f 1, BaP co‐exposure with Der f 1 led to airway hyper‐responsiveness and increased lung inflammation in mouse model of asthma. Increased expression of TSLP, IL‐33, and IL‐25 was also found in the airways of these mice. Moreover, BaP co‐exposure with Der f 1 activated AhR signaling with increased expression of AhR and CYP1A1 and promoted airway epithelial ROS generation and TSLP and IL‐33, but not IL‐25, expression. Interestingly, AhR antagonist CH223191 or cells with AhR knockdown abrogated the increased expression of ROS, TSLP, and IL‐33. Furthermore, ROS inhibitor N‐acetyl‐L‐cysteine (NAC) also suppressed BaP co‐exposure‐induced expression of epithelial TSLP, IL‐33, and IL‐25. Finally, AhR antagonist CH223191 and NAC inhibited BaP co‐exposure with Der f 1‐induced lung inflammation. Conclusions Our findings suggest that BaP facilitates Der f 1‐induced epithelial cytokine release through the AhR‐ROS axis.

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Diesel exhaust and house dust mite allergen lead to common changes in the airway methylome and hydroxymethylome

Cited by 41 publications

References 84 publications

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

A WAO — ARIA — GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020

Prospects and challenges of multi-omics data integration in toxicology

Benzo(a)pyrene facilitates dermatophagoides group 1 (Der f 1)‐induced epithelial cytokine release through aryl hydrocarbon receptor in asthma

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