Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil

Abstract

Petroleum based products are the major source of energy for industries and daily life. Leaks and accidental spills occur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleum products. In the present study we isolated the bacteria from diesel contaminated soil and screened them for diesel biodegradation capacity. One monoculture isolate identified by 16S rRNA gene sequence analysis to be Acinetobacter baumannii was further studied for diesel oil biodegradation. The effects of various culture parameters (pH, temperature, NaCl concentrations, initial hydrocarbon concentration, initial inoculum size, role of chemical surfactant, and role of carbon and nitrogen sources) on biodegradation of diesel oil were evaluated. Optimal diesel oil biodegradation by A. baumanii occurred at initial pH 7, 35°C and initial hydrocarbon concentration at 4%. The biodegradation products under optimal cultural conditions were analyzed by GC-MS. The present study suggests that A. baumannii can be used for effective degradation of diesel oil from industrial effluents contaminated with diesel oil.

Isolation and Screening of Diesel Degrading Bacteria from Ship Dismantling Facility at Tanjungjati, Madura, Indonesia

“…Diesel is a seawater and soil contaminant [4]. Worldwide, about 1.7-8.8 million metric tons of diesel are released into the aquatic environment and soil each year [5].…”

Effect of Different Organic and Inorganic Nitrogen Sources on the Kinetics of the Breakdown of Crude Oil Using Pseudomonas

“…18: Graph of the inverse of the rate of breakdown of TPH using Pseudomonas sp. and ammonium salt against the inverse of the substrate concentration sources need to be introduced, since the pollutants (crude oil) acts as a carbon source (Palanisamy et al, 2014). This present study sets out to find the effect of different nitrogen sources on the biodegradation ability of Pseudomonas sp.…”

Isolation and identification of bacterial consortia responsible for degrading oil spills from the coastal area of Yanbu, Saudi Arabia

“…[34] Similar GCÀMS analysis has been performed after degradation of oil by A. baumannii. [12] In another work, isolated bacterial organisms were able to reduce at least 50% of the relative abundance of various hydrocarbons present in crude oil compared to control as evaluated by GCÀMS analysis. [13] Future research should aim at identification and study of other groups of microorganisms that can act on long-chain hydrocarbons, such as soil fungi and actinomycetes, in the Yanbu area.…”

“…can degrade more than 50% of hydrocarbons present in the environment. [12,13] In Saudi Arabia, the petroleum sector accounts for roughly 45% of the budget revenues, 55% of the gross domestic product (GDP) and 90% of the export earnings according the 2014 statistics. On the other hand, Saudi Arabia has an extended coastline on the Arabian Gulf (also known as Persian Gulf) and the Red Sea, where most oil and petrochemical products are exported, viz.…”