Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Hamidi, Negin; Meamar, Ahmad Reza; Akhlaghi, Lame; Rampisheh, Zahra; Razmjou, Elham

doi:10.3855/jidc.9643

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…In the present study, when we compared age, gender and common GI symptoms between the four groups, no statistically significant difference was noted. A number of studies reported no relation between gender and Blastocystis infection, as well as D. fragilis infection, supporting our findings (33,34). A case control study reported that GI symptoms were more common in cases without D. fragilis or Blastocystis.…”

Section: Discussionsupporting

confidence: 90%

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The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings

Malatyalı

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Tileklioğlu

et al. 2021

Journal of Health Sciences and Medicine

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Aim: Blastocystis and Dientamoeba fragilis (D. fragilis) are among the most common protozoon species in human faecal samples. The cross-sectional studies have reported the frequencies in a variety of populations. However, we have very limited information about the co-existence rate of those protozoans. The study aimed to compare D. fragilis frequency in Blastocystis positive and negative faecal samples in order to determine the co-existence rate. The secondary objective was to analyse demographic characteristics and gastrointestinal (GI) symptoms in relation to both infections. Material and Method: In the present study, we defined a study group that included 100 Blastocystis positive faecal samples and a control group that included 100 Blastocystis negative samples. The frequency of D. fragilis in samples was determined with a PCR assay specific to the small-subunit ribosomal RNA (SS rRNA) gene. A positive control of D. fragilis was used and the samples with amplification of the expected size (863 bp) were considered as positive. In addition to the statistical comparison of frequencies, the descriptive and clinical findings of cases were analysed retrospectively with Pearson chi-square or ANOVA tests. Results: The frequency of D. fragilis was 21% in Blastocystis positive group and it was 10% in Blastocystis negative group. There was statistically significant difference in terms of D. fragilis positivity between the groups (p <0.05). Age, gender and GI symptoms did not reveal a significant difference between the following groups: only Blastocystis infected (n=77), only D. fragilis infected (n=11), infected with both protozoans (n=34) and non-infected individuals (n=89) (p >0.05). Conclusion:Our study highlighted the high co-existence of D. fragilis and Blastocystis in human faecal samples. A possible explanation for this finding may be the faecal-oral transmission of these protozoans. In addition, analysis of clinical findings was supported common asymptomatic colonisation of Blastocystis and D. fragilis.

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Section: Discussionsupporting

confidence: 90%

“…A study reported frequency of D. fragilis with direct smear, formalin-ether concentration, culture, permanent staining and amplification of SSU rRNA and 5.8S rRNA genes. The positive rates with the methods were as follows: 0%, 0%, 1%, 5%, 6% and 13.5%, respectively (33).…”

Section: Discussionmentioning

confidence: 92%

The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings

Malatyalı

Yıldız

Tileklioğlu

et al. 2021

Journal of Health Sciences and Medicine

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“…Permanently staining methods or cultivation procedures are often required to achieve a correct parasitological diagnosis. This fact confirms that the nucleic acidbased assays have been the diagnostic method of choice for D. fragilis in feces [10].…”

Section: Introductionsupporting

confidence: 72%

Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study

et al. 2020

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In most developing countries, Dientamoeba fragilis infection is an obscure protozoan infection. We aimed to determine a frequency and clinical importance of D. fragilis infection in Taif, Saudi Arabia. A 1-year case control study included patients with gastrointestinal (cases, n=114) or non-gastrointestinal symptoms (controls, n=90). The fecal samples were examined with the classical parasitological methods for intestinal protozoa, and by real time PCR for D. fragilis. The infection by D. fragilis was detected in 5.8% by PCR and in 4.4% patients by microscopy. The infection was identified more in control group (n=9) than in cases (n=3); a sole infection in 11 patients and mixed with Giardia in 1 patient. The other enteric parasites detected were Blastocystis sp. (8.3%), Giardia sp. (5.3%), Cryptosporidium sp. (2.9%), Entamoeba histolytica (1.4%), Entamoeba coli (0.9%) and Hymenolepis nana (0.4%). Our results tend to reinforce the need to increase awareness of D. fragilis infection in Saudi Arabia.

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“…[ 14 ] On the contrary, Dientamoeba was not found by direct smear or formalin-ether concentration methods as stated by Hamidi et al . [ 24 ] Previous studies reported that identification and differentiation of Dientamoeba by direct smear and formalin-ether concentration techniques lack accuracy and are laborious and time-consuming compared to molecular assays. [ 25 26 ]…”

Section: Discussionmentioning

confidence: 99%

Evaluation of microscopy and PCR for detection of Dientamoeba fragilis

et al. 2022

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Introduction: Dientamoeba fragilis ( D. fragilis ) diagnosis is an intestinal protozoan parasite globally found in rural and urban areas and is attracting a growing interest. Its prevalence in stool varies from 0.2% to more than 19% depending upon the population studied. Materials and Methods: This study was based on the examination of 100 stool samples of randomly referred cases in a rural area in Motobus district, Kafr El-Sheikh governorate, Egypt. Our aim was to investigate the presence of D. fragilis in stool of the examined individuals using conventional polymerase chain reaction (PCR) compared to wet mount and trichrome stain with confirmation of infection by transmission electron microscopy. Results: D. fragilis was detected in 13/100 of the stool samples examined using wet mount smears, while trichrome stain detected 17/100. Conventional PCR diagnosed 41 cases of D. fragilis in the studied group. A very good agreement was found between wet mount and trichrome stain for diagnosing D. fragilis , while there was fair agreement between conventional PCR and both microscopy methods. Transmission electron microscope was performed on pooled positive samples that revealed the internal structures of D. fragilis trophozoite with its characteristic nucleus. Conclusions: PCR technique was superior to microscopy for the detection of D. fragilis . Trichrome stain remains vital for microscopic diagnosis.

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Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Cited by 6 publications

References 40 publications

The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings

The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings

Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study

Evaluation of microscopy and PCR for detection of Dientamoeba fragilis

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