Dielectrophoretic trapping and impedance detection of Escherichia coli, Vibrio cholera, and Enterococci bacteria

Kong, Tian Fook; Shen, Xiaojun; Marcos, .; Yang, Chun; Ibrahim, I.H.

doi:10.1063/5.0024826

Cited by 15 publications

(13 citation statements)

References 23 publications

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“…Although surface motility can be a crucial strategy in bacterial growth whereby bacteria seek nutrients and enable new colonies [39], the present results emphasize this mechanism by which the necessity of the affinity of pili to the substrate is required. Overall, the study provides comprehensive understanding of bacterial-SU-8 surface interaction which could lead to design effective strategies for the fabrication of SU-8 based various unique devices for biomedical applications [8][9][10][11]14].…”

Section: B)mentioning

confidence: 99%

“…The SU-8 epoxy polymer is one such material that has great potential for fabrication of high aspect ratio of micro/nanostructured scaffolds for lab-on-a-chip devices [1][2][3][4]. In particular, SU-8 has been used as an impressive platform for the development of various smart biomedical devices including biosensors [5][6][7], bacterial diagnosis [8][9][10], cantilever [11], bioelectrodes [12] and microrobots [13,14] owing to its excellent optical and mechanical properties with chemical stability even against acids [3,15,16]. Moreover, SU-8 nanostructures with tunable high aspect ratios have been fabricated and used for eukaryote cell interface and for simultaneous visualization of the traction force and focal adhesion of eukaryotic cells [17].…”

Section: Introductionmentioning

confidence: 99%

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Physiochemically distinct SU-8 surfaces tailor Xylella fastidiosa cell-surface holdfast and colonization

Anbumani

Silva

Alaferdov

et al. 2021

Preprint

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SU-8 polymer is an excellent platform for diverse applications due to its high aspect ratio of micro/nanostructures fabrication and exceptional optical, chemical, and biocompatible properties. Although SU-8 has been often investigated for a variety of biological applications, how its surface properties influence both the interaction of bacterial cells with the substrate and its colonization is poorly understood. In this work, we tailor SU-8 nanoscale surface properties to investigate single cell motility, adhesion and successive colonization of a phytopathogenic bacteria, Xylella fastidiosa. Different surface properties of SU-8 thin films have been prepared using photolithography processing and oxygen plasma treatment. We found a significant difference in bacterial cell behavior and subsequent colonization on SU-8 as surface property changes. A larger density of carboxyl groups in hydrophilic plasma-treated SU-8 surfaces promotes faster cell motility in the earlier stage of the growth. The hydrophobic nature of pristine SU-8 surfaces has no trackable bacterial motility with 5 to 10 times more single cells adhered to surface than its plasma-treated counterpart. In fact, plasma-treated SU-8 samples suppressed bacterial adhesion, with surfaces showing less than 5% coverage. These results not only showcase that SU-8 surface properties can impact the bacterial behavior in a spatiotemporal manner, but also provide insights on the prominent ability of pathogens to evolve and adapt to different surface properties.

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Section: B)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physiochemically distinct SU-8 surfaces tailor Xylella fastidiosa cell-surface holdfast and colonization

Anbumani

Silva

Alaferdov

et al. 2021

Preprint

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“…The ability to bacterial or human cancer cell pearl chain, down to the single cell level, would enable a robust platform for fundamental studies and clinical applications such as investigation of drug uptake or resistance, on-chip single cell labeling, and cell to cell adhesion strength. One of the applications for bacterium trapping and isolation through DEP was demonstrated to facilitate integration of immunoassay and detection of target microorganism [11,14]. Another intriguing application of cell DEP for single cell isolation of cancer cells through negative DEP was to measure and characterize the cell impedance for identification of the pathological stages of cancer of MDA-MB-231 and Michigan Cancer Foundation-7 (MCF-7) [15].…”

Section: Introductionmentioning

confidence: 99%

Bacteria and cancer cell pearl chain under dielectrophoresis

et al. 2021

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In this work, we aim to observe and study the physics of bacteria and cancer cells pearl chain formation under dielectrophoresis (DEP). Experimentally, we visualized the formation of Bacillus subtilis bacterial pearl chain and human breast cancer cell (MCF-7) chain under positive and negative dielectrophoretic force, respectively. Through a simple simulation with creeping flow, AC/DC electric fields, and particle tracing modules in COMSOL, we examined the mechanism by which bacteria self-organize into a pearl chain across the gap between two electrodes via DEP. Our simulation results reveal that the region of greatest positive DEP force shifts from the electrode edge to the leading edge of the pearl chain, thus guiding the trajectories of free-flowing particles toward the leading edge via positive DEP. Our findings additionally highlight the mechanism why the free-flowing particles are more likely to join the existing pearl chain rather than starting a new pearl chain. This phenomenon is primarily due to the increase in magnitude of electric field gradient, and hence DEP force exerted, with the shortening gap between the pearl chain leading edge and the adjacent electrode. The findings shed light on the observed behavior of preferential pearl chain formation across electrode gaps.

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“…24 The forces utilized to achieve medium exchange are manifold. The most commonly used forces are dielectric, 12,[25][26][27][28] magnetic, 6,9,13,22,23 hydrodynamic, 18,29,30 and acoustic. 16,19,27,[31][32][33][34] However, each of these methods exhibits fundamental limitations, such as the requirement of low flow rates or particle labelling.…”

Section: Introductionmentioning

confidence: 99%

Acoustofluidic Medium Exchange for Preparation of Electrocompetent Bacteria Using Channel Wall Trapping

Gerlt

Ruppen

Leuthner

et al. 2021

Preprint

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Transformation, i.e. reprogramming of bacteria by delivering exogenous genetic material (such as DNA) into the cytoplasm, is a key process in molecular engineering and modern biotechnology in general. Transformation is often performed by electroporation, i.e. creating pores in the membrane using electric shocks in a low conductivity environment. However, cell preparation for electroporation can be cumbersome as it requires the exchange of growth medium (high-conductivity) for low-conductivity medium, typically performed via multiple time-intensive centrifugation steps. To simplify and miniaturize this step, we developed an acoustofluidic device capable of trapping the bacterium Escherichia coli non-invasively for subsequent exchange of medium, which is challenging in acoustofludic devices due to detrimental acoustic streaming effects. With an improved etching process, we were able to produce a thin wall between two microfluidic channels, which, upon excitation, can generate streaming fields that complement the acoustic radiation force and therefore can be utilized for trapping of bacteria. Our novel design robustly traps Escherichia coli at a flow rate of 10 µL minute-1 and has a cell recovery performance of 47 ± 3 % after washing the trapped cells. To verify that the performance of the medium exchange device is sufficient, we tested the electrocompetence of the recovered cells in a standard transformation procedure and found a transformation efficiency of 8∙105 CFU per µg of plasmid DNA. Our device is a viable low-volume alternative to centrifugation-based methods and opens the door for miniaturization of a plethora of microbiological and molecular engineering protocols.

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Dielectrophoretic trapping and impedance detection of Escherichia coli, Vibrio cholera, and Enterococci bacteria

Abstract: Dielectrophoretic trapping and impedance detection of Escherichia coli, Vibrio cholera, and Enterococci bacteria

Cited by 15 publications

References 23 publications

Physiochemically distinct SU-8 surfaces tailor Xylella fastidiosa cell-surface holdfast and colonization

Physiochemically distinct SU-8 surfaces tailor Xylella fastidiosa cell-surface holdfast and colonization

Bacteria and cancer cell pearl chain under dielectrophoresis

Acoustofluidic Medium Exchange for Preparation of Electrocompetent Bacteria Using Channel Wall Trapping

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