Dielectrophoretic separation of platelet cells in a microfluidic channel and optimization with fuzzy logic

Ertugrul, İshak; Ülkir, Osman

doi:10.1039/d0ra06271e

Cited by 19 publications

(11 citation statements)

References 31 publications

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“…In COMSOL simulation, we chose the boundaries as a wall with no specific material property for the microchip. Water was selected as fluid [25], and solid particles have a 5µm diameter as an MK.…”

Section: Comsol Multiphysics Implementationmentioning

confidence: 99%

Optimization of megakaryocyte trapping for platelet formation in microchannels

Baydar-Atak

İnsel

Oruc

et al. 2022

CI&CEQ

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Platelets (PLTs) are responsible for stopping the bleeding. They are small cell fragments produced from megakaryocytes (MKs) in the bone marrow. Low platelet count is a significant health problem for a patient. PLTs can usually be stored for up to 5 days prior to transfusion. Instantaneous production of PLTs from isolated and stored MKs is crucial for the patient?s health. Thanks to microfluidic platforms, PLTs can be produced instantaneously from MKs. Herein, we have computationally studied fluid dynamics in the microchannels with slit structures and different inlet geometries. Analysis of the flow dynamics was performed by the commercial analysis software. The effects of flow rates and the angle between the inlet channels on the MKs trapping were investigated. The optimization of the angle between inlet channels and flow rates of main and pressure flows was done with Response Surface Methodology (RSM) by counting the trapped MKs. The optimum conditions lead to the percentage of trapped MKs were 100 with a relative deviation of <1%. We also concluded that flow rates to trapping a higher amount of MKs are as important as the angle between the inlet channels.

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Section: Comsol Multiphysics Implementationmentioning

confidence: 99%

Optimization of megakaryocyte trapping for platelet formation in microchannels

Baydar-Atak

İnsel

Oruc

et al. 2022

CI&CEQ

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“…If we develop a technique that can manipulate multiple target pieces individually and combine them with identification meanings (e.g., by visible and infrared light), most of the complexities involving sorting by physical properties can be removed. Applications of electrostatic forces other than plastic sorting include dust control on solar panels on the earth [2][3][4] and lunar surface [5], trapping plenty of particles onto insulator surfaces [6], sorting, trapping, traveling of living cells, and microparticles in the liquid phase [7][8][9][10][11][12][13][14][15][16][17][18][19]. Representative device structures are (a) a plane substrate with interdigitated electrodes on it [3][4][5][6]8,14,16,18], (b) a micro channel with multiple electrodes along it [7,9], and (c) a micro chamber surrounded by a set of electrodes on a plane [19].…”

Section: Introductionmentioning

confidence: 99%

“…Applications of electrostatic forces other than plastic sorting include dust control on solar panels on the earth [2][3][4] and lunar surface [5], trapping plenty of particles onto insulator surfaces [6], sorting, trapping, traveling of living cells, and microparticles in the liquid phase [7][8][9][10][11][12][13][14][15][16][17][18][19]. Representative device structures are (a) a plane substrate with interdigitated electrodes on it [3][4][5][6]8,14,16,18], (b) a micro channel with multiple electrodes along it [7,9], and (c) a micro chamber surrounded by a set of electrodes on a plane [19]. They apply phase-controlled AC voltage to treat target pieces not individually but as a mass.…”

Section: Introductionmentioning

confidence: 99%

Foundation of the Manipulation Technology for Tiny Objects Based on the Control of the Heterogeneity of Electric Fields

Shirota

Yoshida

2022

Energies

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Effective sorting and extraction of tiny plastic objects is becoming increasingly important for manufacturing high-quality recycled plastics. Herein, we designed a manipulation device for tiny objects that can drive multiple target objects individually. This type of device has a potential to sort tiny pieces of a wide variety of materials, not strongly depending on their physical properties, by combining different detection meanings. In this study, two types of devices were tested as the basic components of the proposed device. One of them had a single object-holding point and the other had two of them. These holding points consisted of strip-shaped electrodes facing each other. The high voltage applied to the facing electrodes created forces heading toward the object-holding points caused by the heterogeneity of the electric field in the devices. The forces created in these devices were determined from the motion analysis of a glass sphere, which is a model for target objects, and a numerical simulation. The results indicate that dielectrophoretic forces are dominant at locations that are sufficiently remote from the holding point, and the Coulombic force caused by dielectric barrier discharge is dominant near the high-voltage electrodes with the holding point. Moreover, the transfer of a glass sphere from one holding point to an adjacent point was successfully demonstrated.

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“…An active microdevice requires an external force to isolate the target cells from the mixture of cells. On the contrary, a passive microdevice does not need any external force, it rather utilizes biophysical laws, geometrical, and hydrodynamic effects for the isolation of target cells. ,, Several active − and passive − microfluidic devices are available in the literature for the isolation of platelets or pure plasma from blood; whereas, only a few microdevices have demonstrated the isolation of a suspension of platelets in the plasma. Among these, most of the reported microdevices are for the enrichment of platelets in the plasma sample ,, and not for the depletion of platelets.…”

Section: Introductionmentioning

confidence: 99%

Biophysical Phenomenon-Based Separation of Platelet-Poor Plasma from Blood

Laxmi

Joshi

Agrawal

2021

Ind. Eng. Chem. Res.

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The extraction or isolation of biological entities on a microfluidic platform has gained considerable attention in disease diagnostics, biomedical research, and point-of-care applications. The isolation of platelet-poor plasma from blood is the first and an essential step for applications in coagulation studies. The widely known clinical methods of platelet-poor plasma separation are based on centrifugation, which require a long processing time, high power, skilled personnel, and are incompatible with point-of-care devices. The microdevice utilized in this study facilitates the employment of various biophysical phenomena, hydrodynamic forces acting on cells, and geometrical features of the microdevice for its operation. The developed microdevice is compact in size, easy to fabricate, reliable, and exhibits clog-free operation. The microdevice isolates platelet-poor plasma with a purity of 94.7 ± 1.90%, having a platelet count of 968 ± 15 per μL plasma, while operating at an inlet flow rate of 0.4 mL/min. Furthermore, biological assessment of the platelet-poor plasma extracted from the microdevice confirms that the sample is completely free from white blood cells and have no impact on the shape and size of the platelets after microfluidic processing.

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Dielectrophoretic separation of platelet cells in a microfluidic channel and optimization with fuzzy logic

Abstract: It is the aim to develop optimization techniques to separate platelets from Red Blood Cells (RBCs) after designing and analyzing a microfluidic chip in this study.

Cited by 19 publications

References 31 publications

Optimization of megakaryocyte trapping for platelet formation in microchannels

Optimization of megakaryocyte trapping for platelet formation in microchannels

Foundation of the Manipulation Technology for Tiny Objects Based on the Control of the Heterogeneity of Electric Fields

Biophysical Phenomenon-Based Separation of Platelet-Poor Plasma from Blood

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