Dielectrophoretic manipulation of particles on a microfluidics platform with planar tilted electrodes

Dalili, Arash; Montazerian, Hossein; Sakthivel, Kabilan; Tasnim, Nishat; Hoorfar, Mina

doi:10.1016/j.snb.2020.129204

Cited by 26 publications

(22 citation statements)

References 36 publications

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“…When the particles enter the DEP separation region, they are displaced by this force according to their size and sorted in the outlets. The design of the DEP separation region is based on the findings of our previous studies [8,14,29]. Although these studies did not show explicitly the DEP‐based separation of a mixture of particles (which is the subject of this paper), their results revealed the criteria required in designing high‐throughput LOC devices for separation of 5, 10, and 15 μm particles from each other.…”

Section: Methodsmentioning

confidence: 98%

“…1B) is 12 μm and 60 μm, respectively, which can apply a strong DEP force on microparticles in the proposed 25 μm tall channel. The electrodes are slanted at 10° (

θ = 10^{\circ}

) with respect to the flow direction, since it is reported that the size‐based separation of microparticles is enhanced at this angle [14]. The length of the DEP region is 7 mm as our experiments with single‐size particles suggested that 5, 10, and 15 μm particles can be positioned at separate regions along the width of the channel [14].…”

Section: Methodsmentioning

confidence: 99%

“…Among the active methods, dielectrophoresis (DEP) has been widely used for the on‐chip manipulation of cells/particles as it is a label‐free and cost‐effective method compared to fluorescently activated cell sorters and magnetically activated cell sorters [2,13]. Furthermore, the elimination of cell labeling makes the DEP preliminary steps less labor‐intensive and costly compared to fluorescence‐activated cell sorting and magnetic cell sorting methods [14].…”

Section: Introductionmentioning

confidence: 99%

“…Since the focusing and separation regions use the same method, the fabrication is easy and cost effective. Also, the effective throughputs for both regions are in the same order and can be combined [14]. So far several studies have used the DEP force for focusing the cells/particles [26–28], among which some have separated cells or particles by the DEP force on the same chip [26,27].…”

Section: Introductionmentioning

confidence: 99%

“…Our recent investigation revealed that the DEP‐induced displacement of the particles increases with the length of the DEP region and decreases with the volumetric throughput. Also, we reported that the electrodes slanted at 10° (with respect to the flow direction) lead to a larger displacement of the particles compared to the electrodes deflected at 45° [14].…”

Section: Introductionmentioning

confidence: 99%

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Sheath‐assisted versus sheathless dielectrophoretic particle separation

Dalili

Hoorfar

2021

Electrophoresis

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Lab‐on‐chip devices are widely being used for binary and ternary cell/particle separation applications. Among the lab‐on‐chip methods, dielectrophoresis (DEP) is a cost‐effective and label‐free method, with great capabilities for size‐based separation of cells and particles, which is mostly performed in sheath‐assisted forms. However, the elimination of the sheath flows offers advantages such as ease of operation and higher sample throughput. In this work, we present a comparison of sheath‐assisted and sheathless DEP separation of three sizes of microparticles using tilted electrodes. The sheath‐assisted design was capable of separating the 5, 10, and 15 μm particles with a separation efficiency as high as 98.0% for 15 μm particles. By adding a DEP focusing region, a sheathless DEP separator was proposed, which offered higher throughputs (up to 10 times) at the cost of lowering the separation efficiency (a reduction up to 10.3% for 15 μm) compared to the sheath‐assisted design. To enhance the separation efficiency, a combination of the DEP focusing accompanied by weak sheath flows from both sides was proposed. This design achieved the highest sample separation yield in the outlets (as high as 98.7% for 15 μm) with a sample throughput of more than 4.2 μL/min. This study provides insights into the choice of an appropriate platform for any application in which the yield, purity, throughput, and portability must be considered.

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Section: Methodsmentioning

confidence: 98%

“…1B) is 12 μm and 60 μm, respectively, which can apply a strong DEP force on microparticles in the proposed 25 μm tall channel. The electrodes are slanted at 10° (

θ = 10^{\circ}

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sheath‐assisted versus sheathless dielectrophoretic particle separation

Dalili

Hoorfar

2021

Electrophoresis

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In vitro dielectrophoresis of HEK cell migration for stimulating chronic wound epithelialization

et al. 2021

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This article describes a dielectrophoresis (DEP)‐based simulation and experimental study of human epidermal keratinocyte (HEK) cells for wounded skin cell migration toward rapid epithelialization. MyDEP is a standalone software designed specifically to study dielectric particles and cell response to an alternating current (AC) electric field. This method demonstrated that negative dielectrophoresis (NDEP) occurs in HEK cells at a wide frequency range in highly conductive medium. The finite element method was used to characterize particle trajectory based on DEP and drag force. The performance of the system was assessed using HEK cells in a highly conductive EpiLife suspending medium. The DEP experiment was performed by applying sinusoidal wave AC potential at the peak‐to‐peak voltage of 10 V in a tapered aluminum microelectrode array from 100 kHz to 1 MHz. We experimentally observed the occurrence of NDEP, which attracted HEK cells toward the local electric field minima in the region of interest. The DIPP‐MotionV software was used to track cell migration in the prerecorded video via an automatic marker and estimate the average speed and acceleration of the cells. The results showed that HEK cell migration was accomplished approximately at 6.43 μm/s at 100 kHz with 10 V, and FDEP caused the cells to migrate and align at the target position, which resulted in faster wound closures because of the application of an electric field frequency to HEK cells in random locations.

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High‐efficiency extraction of target particles in viscoelastic contraction–expansion microchannels

Wu,

Liu,

Jiang

et al. 2023

Electrophoresis

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The efficient and precise extraction of target particles is a crucial prerequisite for achieving accurate detection and analysis in microfluidic cell analysis. In this study, a symmetrical contraction–expansion microchannel with sheath flow was designed, aiming to extract target larger particles from particles of different sizes within the channel. This paper conducted numerical simulations to investigate the three‐dimensional migration mechanisms of particles and performed experimental studies to examine the separation performance of particles with different sizes under varying flow rate ratios and different numbers of contraction–expansion structures. The experimental results indicate that at moderate sample flow rates and higher flow rate ratios, microchannels with fewer contraction–expansion structures are likely to achieve better performance in extracting target particles compared to microchannels with a greater number of these structures. Our work advances the application of viscoelastic contraction–expansion microchannels in particle separation. This device is easy to set up in parallel and significantly enhances throughput, providing an accurate and efficient solution for future particle separation applications.

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Dielectrophoretic manipulation of particles on a microfluidics platform with planar tilted electrodes

Cited by 26 publications

References 36 publications

Sheath‐assisted versus sheathless dielectrophoretic particle separation

Sheath‐assisted versus sheathless dielectrophoretic particle separation

In vitro dielectrophoresis of HEK cell migration for stimulating chronic wound epithelialization

High‐efficiency extraction of target particles in viscoelastic contraction–expansion microchannels

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