Dielectrophoretic detection of electrical property changes of stored human red blood cells

Lavi, Edwin D.; Crivellari, Francesca; Gagnon, Zachary

doi:10.1002/elps.202100241

Cited by 8 publications

(11 citation statements)

References 22 publications

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“…Technologies based on the dielectrophoresis effects are effective for the analysis of fatigue, force, and stress at the cellular level. For example, cell manipulation systems have been widely employed to measure cellular biomechanics using microfluidic platforms, including studies on cell stretching and manipulation [135,136], electrical property changes of stored RBC [137], label-free and noninvasive characterization for the viscoelastic properties of RBC [138],benchmarking dielectrophoretic separation metrics of unknown types of RBC (healthy, modified, …) [139], the oxidative stress analysis for RBCs (Figure 9A) [140], dynamic fatigue measurements [141], detecting circadian rhythms in RBCs [142], nonlinear viscoelastic analyses (Figure 9B) [143], biomechanics of erythrocyte membrane failures [144], liquid metal electrode-based dielectrophoretic schemes [145], and a portable system with multiple dielectrophoretic applications for RBC analyses [146].…”

Section: Rbc Dielectrophoretic Analysismentioning

confidence: 99%

Section: Rbc Dielectrophoretic Analysismentioning

confidence: 99%

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Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

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Section: Rbc Dielectrophoretic Analysismentioning

confidence: 99%

Section: Rbc Dielectrophoretic Analysismentioning

confidence: 99%

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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“…The excellent reproducibility, accurate measurements, low cost, and short response time of this paper-based potentiometric device illustrate a promising future for single-use portable biosensors in clinical applications. Reusable systems for the assessment of ions in blood have also been developed, and examples are the CE-based system by Zhao et al [22] and the dielectrophoretic-based sensor used for electrical characterization of stored blood samples by Lavi et al [23].…”

Section: Determination Of Ions Sugars and Metabolites From Bodily Flu...mentioning

confidence: 99%

“…[22] and the dielectrophoretic‐based sensor used for electrical characterization of stored blood samples by Lavi et al. [23].…”

Section: Microfluidics For the Analysis Of Ions And Small Moleculesmentioning

confidence: 99%

Microfluidic systems in clinical diagnosis

Lapizco‐Encinas

Zhang

2022

Electrophoresis

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The use of microfluidic devices is highly attractive in the field of biomedical and clinical assessments, as their portability and fast response time have become crucial in providing opportune therapeutic treatments to patients. The applications of microfluidics in clinical diagnosis and point‐of‐care devices are continuously growing. The present review article discusses three main fields where miniaturized devices are successfully employed in clinical applications. The quantification of ions, sugars, and small metabolites is examined considering the analysis of bodily fluids samples and the quantification of this type of analytes employing real‐time wearable devices. The discussion covers the level of maturity that the devices have reached as well as cost‐effectiveness. The analysis of proteins with clinical relevance is presented and organized by the function of the proteins. The last section covers devices that can perform single‐cell metabolomic and proteomic assessments. Each section discusses several strategically selected recent reports on microfluidic devices successfully employed for clinical assessments, to provide the reader with a wide overview of the plethora of novel systems and microdevices developed in the last 5 years. In each section, the novel aspects and main contributions of each reviewed report are highlighted. Finally, the conclusions and future outlook section present a summary and speculate on the future direction of the field of miniaturized devices for clinical applications.

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“…Sufficient cell focusing is meaningful in all flow cytometers, including microflow cytometers [14]. Various field‐assisted techniques reported utilizable in cell focusing in microchannel, including force [15], electric [16], magnetic [17], ultrasonic [18], and thermal fields [19]. For example, electrokinetically pumped sheath flows were used by Dovichi's group to focus and transfer separated analytes from capillary to mass spectrometry [20].…”

Section: Introductionmentioning

confidence: 99%

Size‐resolved counting of circulating tumor cells on pinched flow‐based microfluidic cytometry

et al. 2022

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Precise cell detecting and counting is meaningful in circulating tumor cells (CTCs) analysis. In this work, a simple cyclic olefin copolymer (COC) microflow cytometer device was developed for size‐resolved CTCs counting. The proposed device is constructed by a counting channel and a pinched injection unit having three channels. Through injection flow rate control, microspheres/cells can be focused into the centerline of the counting channel. Polystyrene microspheres of 3, 9, 15, and 20 µm were used for the microspheres focusing characterization. After coupling to laser‐induced fluorescence detection technique, the proposed device was used for polystyrene microspheres counting and sizing. A count accuracy up to 97.6% was obtained for microspheres. Moreover, the proposed microflow cytometer was applied to CTCs detecting and counting. To mimic blood sample containing CTCs and CTCs mixture with different subtypes, an MDA‐MB‐231 (human breast cell line) spiked red blood cells sample and a mixture of MDA‐MB‐231 and MCF‐7 (human breast cell line) sample were prepared, respectively, and then analyzed by the developed pinched flow‐based microfluidic cytometry. The simple fabricated and easy operating COC microflow cytometer exhibits the potential in the point‐of‐care clinical application.

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Dielectrophoretic detection of electrical property changes of stored human red blood cells

Cited by 8 publications

References 22 publications

Advances in Microfluidics for Single Red Blood Cell Analysis

Advances in Microfluidics for Single Red Blood Cell Analysis

Microfluidic systems in clinical diagnosis

Size‐resolved counting of circulating tumor cells on pinched flow‐based microfluidic cytometry

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