Dielectrophoretic chromatography with cross-flow injection

Sano, Hironari; Kabata, Hiroyuki; Kurosawa, Osamu; Washizu, Masao

doi:10.1109/memsys.2002.984043

Cited by 10 publications

(14 citation statements)

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“…They have reported numerous studies on the manipulation of DNA by DEP and EOF, where special interest is given to methods for cutting target fragments of DNA ranging from the application of high intensity UV light and laser [9][10][11] and mechanical cutting [12,13], to the employment of immobilized enzymes [14,15]. They have also carried out the DEP chromatography of DNA [16][17][18], measured the collection ratio of DNA and demonstrated the use of DEP enhancers to achieve the separation of molecules of similar size [17]. A review of their work was written by Washizu in 2005 [19].…”

Section: Washizu and Co-workersmentioning

confidence: 99%

“…They utilized microchannels that had 250 electrodes in a corrugated shape. Applying a voltage of 5 V they were able to achieve a clear separation between these two types of DNA that are closer in size, by obtaining clearly separated chromatographic peaks, the 6.6 kbp DNA peak was observed at 100 s and the 48.5 kbp DNA peak was observed at 155 s [18].…”

Section: Washizu and Co-workersmentioning

confidence: 99%

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Dielectrophoresis for the manipulation of nanobioparticles

2007

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Dielectrophoresis (DEP) is a nondestructive electrokinetic mechanism with great potential for the manipulation of bioparticles. DEP is the movement of particles induced by polarization effects in nonuniform electric fields. Since the 1960s, this technique has been successfully used for the manipulation of microbioparticles, such as microorganisms. Moreover, due to the advances in microfabrication techniques, that allowed progressively smaller microstructures to be constructed, DEP can now be used for the manipulation of nanobioparticles. The first research studies on the DEP of nanobioparticles started in the 1990s. Since then, many research groups have carried out outstanding work with DEP of nanobioparticles such as macromolecules, virus, and spores. However, the need of a critical report that integrates these findings is evident. The aim of the present review is to depict the state-of-the-art on the use of DEP for the separation of nanobioparticles and the potential trends of novel applications of this technique. This review compiles and analyzes the significant findings obtained by many researchers. This publication is intended to provide the reader with state-of-the-art information on many research studies focused on DEP to handle nanobioparticles.

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Section: Washizu and Co-workersmentioning

confidence: 99%

Section: Washizu and Co-workersmentioning

confidence: 99%

Dielectrophoresis for the manipulation of nanobioparticles

2007

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“…Apart from established DEP concepts such as field-flow fractionation [17,18], filtration [19], selective trapping (e.g., insulator-based dielectrophoresis) [20], dielectrophoretic particle chromatography (DPC) is a promising concept to achieve high throughput separation of particles. Since DPC was introduced by Washizu et al [5], different approaches were done using selective trapping of particles [21,22], packed bed columns [23], or stepwise change of the frequency [24]. DEP chromatography proved to be very successful in isolating circulating breast tumor cells (CTCs) from blood [25] at a very low concentration.…”

Section: Introductionmentioning

confidence: 99%

Polarizability-Dependent Sorting of Microparticles Using Continuous-Flow Dielectrophoretic Chromatography with a Frequency Modulation Method

et al. 2019

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The separation of microparticles with respect to different properties such as size and material is a research field of great interest. Dielectrophoresis, a phenomenon that is capable of addressing multiple particle properties at once, can be used to perform a chromatographic separation. However, the selectivity of current dielectrophoretic particle chromatography (DPC) techniques is limited. Here, we show a new approach for DPC based on differences in the dielectrophoretic mobilities and the crossover frequencies of polystyrene particles. Both differences are addressed by modulating the frequency of the electric field to generate positive and negative dielectrophoretic movement to achieve multiple trap-and-release cycles of the particles. A chromatographic separation of different particle sizes revealed the voltage dependency of this method. Additionally, we showed the frequency bandwidth influence on separation using one example. The DPC method developed was tested with model particles, but offers possibilities to separate a broad range of plastic and metal microparticles or cells and to overcome currently existing limitations in selectivity.

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“…insulator-based dielectrophoresis) [20], dielectrophoretic particle chromatography (DPC) is a promising concept to achieve high-throughput separation of particles. Since DPC was introduced by Washizu et al [5], different approaches were made using selective trapping of particles [21,22], packed bed columns [23] or stepwise change of the frequency [24]. DEP chromatography proofed to be very successful in isolating circulating breast tumor cells (CTCs) from blood [25] at very low concentration.…”

Section: Introductionmentioning

confidence: 99%

Polarizability-dependent Sorting of Microparticles Using Continuous-flow Dielectrophoretic Chromatography with a Frequency Modulation Method

Giesler

Pesch

Weirauch

et al. 2019

Preprint

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The separation of microparticles with respect to different properties such as size and material is a research field of great interest. Dielectrophoresis, a phenomenon which is capable of addressing multiple particle properties at once, can be used to perform a chromatographic separation. However, the selectivity of current dielectrophoretic particle chromatography (DPC) techniques is limited. Here we show a new approach for DPC based on differences in the dielectrophoretic mobilities and the crossover frequencies of polystyrene particles. Both differences are addressed by modulating the frequency of the electric field to generate positive and negative dielectrophoretic movement to achieve multiple trap and release cycles of the particles. A chromatographic separation of different particle sizes revealed a voltage dependency of this method. Additionally, we showed the frequency bandwidth influence on separation using one example. The DPC method developed was tested with model particles but offers possibilities to separate a broad range of plastic and metal microparticles or cells and to overcome currently existing limitations in selectivity.

show abstract

Dielectrophoretic chromatography with cross-flow injection

Cited by 10 publications

References 1 publication

Dielectrophoresis for the manipulation of nanobioparticles

Dielectrophoresis for the manipulation of nanobioparticles

Polarizability-Dependent Sorting of Microparticles Using Continuous-Flow Dielectrophoretic Chromatography with a Frequency Modulation Method

Polarizability-dependent Sorting of Microparticles Using Continuous-flow Dielectrophoretic Chromatography with a Frequency Modulation Method

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