Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz

Chung, Colin; Waterfall, Martin; Pells, Steve; Menachery, Anoop; Smith, S.; Pethig, Ronald

doi:10.5617/jeb.196

Cited by 34 publications

(24 citation statements)

References 16 publications

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“…Detailed experimental measurements of f xo2 for mammalian cells are, to our knowledge, restricted to an earlier report of our own for murine myeloma cells . Values for f xo2 were observed in the region of 200 MHz, in agreement with theory based on known cell dielectric parameters.…”

Section: Introductionsupporting

confidence: 78%

“…In previous work we described how the value of f xo2 for cells suspended in DEP medium gradually decreased over time. The initial decrease of f xo2 , at 19 MHz/h at 21°C for the first 2 h, limited the time that the cells could be suspended.…”

Section: Methodsmentioning

confidence: 99%

“…The device used for DEP characterisation is described fully elsewhere . In brief, an array of sixteen 100 nm thick, 20 μm wide, platinum interdigitated electrodes spaced 40 μm apart were vacuum deposited onto a glass substrate.…”

Section: Methodsmentioning

confidence: 99%

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Intracellular potassium under osmotic stress determines the dielectrophoresis cross‐over frequency of murine myeloma cells in the MHz range

et al. 2018

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Dielectrophoresis (DEP) has been widely studied for its potential as a biomarker-free method of sorting and characterizing cells based upon their dielectric properties. Most studies have employed voltage signals from ∼1 kHz to no higher than ∼30 MHz. Within this range a transition from negative to positive DEP can be observed at the cross-over frequency f . The value of f is determined by the conductivity of the suspending medium, as well as the size and shape of the cell and the dielectric properties (capacitance, conductivity) of its plasma membrane. In this work DEP measurements were performed up to 400 MHz, where the transition from positive to negative DEP can be observed at a higher cross-over frequency f . SP2/O murine myeloma cells were suspended in buffer media of different osmolarities and measurements taken of cell volume, f and f . Potassium-binding benzofuran isophthalate (PBFI), a potassium-sensitive fluorophore, and flow cytometry was employed to monitor relative changes in intracellular potassium concentration. In agreement with theory, it was found that f is independent of the cell parameters that control f and is predominantly determined by intracellular conductivity. In particular, the value of f is highly correlated to that of the intracellular potassium concentration.

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Section: Introductionsupporting

confidence: 78%

Section: Methodsmentioning

confidence: 99%

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Intracellular potassium under osmotic stress determines the dielectrophoresis cross‐over frequency of murine myeloma cells in the MHz range

et al. 2018

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“…The estimation of low frequency is useful in the case of measurement inside a less conductive medium and where the peak frequency is adequately low. Furthermore, in the experiments with a higher peak frequency like 100 MHz these estimations are invalid for attaining the dielectric characteristics of cells [10]. Thus, this theory is in most situations not suitable for cell dielectric characterization from measured data.…”

Section: Introductionmentioning

confidence: 96%

Application of genetic algorithm in extracting cell dielectric characteristics with electrorotation

Alizadeh-Haghighi

Jafarmadar

Khalilarya

2017

Journal of Electrical Bioimpedance

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In this study the transformed theory is applied to derive the dielectric characteristics of cells, considering the electrorotation (ER) peak frequency. In current studies, estimations of low frequency, which are credible for the values less than 1 mS/m for medium conductivity, are used to obtain the corresponding permittivity and conductivity of cells. Unlike the presented works, the transformed theory applies the comprehensive statement for corresponding permittivity and conductivity of cells. In the transformed theory, the membrane and interior characteristics could be obtained from the high and the low frequencies of peak ER, for all values of conductivity of medium. Characteristics of cells are obtained via optimization of an equation for the conductivity of medium regarding the peak ER frequency. The optimization process is performed applying genetic algorithm due to its swift adaptation to the problem and faster convergence.

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“…Temperature also affects the upper crossover frequency, e.g. temperature of 37°C drops the second crossover frequency of cell samples from 200 MHz to 90 MHz in two hours . The results show that the proposed iDEP creek‐gap device could evaluate the value of Re( β ) for a wide range of field frequencies.…”

mentioning

confidence: 93%

Insulator‐based dielectrophoresis combined with the isomotive AC electric field and applied to single cell analysis

2019

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We developed an insulator‐based dielectrophoretic (iDEP) creek‐gap device that enables the isomotive movement of cells and that is suitable for determining their DEP properties. In the iDEP creek‐gap device, a pair of planar insulators forming a single fan‐shaped channel allows the induction of the isomotive iDEP force on cells. Hence, the cells’ behavior is characterized by straight motion at constant velocity in the longitudinal direction of the channel. Operation of the device was demonstrated using human breast epithelial cells (MCF10A) by applying an AC voltage of Vpp = 34 V peak‐to‐peak and frequencies of 200 kHz and 50 MHz to the device. Subsequently, the magnitude of DEP forces and the real part of the ClausiusMossotti (CM) factor, Re(β), were deduced from the measured cell velocity. The values of Re(β) were 0.14 ± 0.01 for the frequency of 200 kHz and −0.12 ± 0.01 for 50 MHz. These results demonstrated that the DEP properties of the cells could be extracted over a wide field frequency range. Therefore, the proposed iDEP creek‐gap device was found to be applicable to cell analysis.

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Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz

Cited by 34 publications

References 16 publications

Intracellular potassium under osmotic stress determines the dielectrophoresis cross‐over frequency of murine myeloma cells in the MHz range

Intracellular potassium under osmotic stress determines the dielectrophoresis cross‐over frequency of murine myeloma cells in the MHz range

Application of genetic algorithm in extracting cell dielectric characteristics with electrorotation

Insulator‐based dielectrophoresis combined with the isomotive AC electric field and applied to single cell analysis

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