Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array

Gel, Murat; Kimura, Yuji; Kurosawa, Osamu; Oana, Hidehiro; Kotera, Hidetoshi; Washizu, Masao

doi:10.1063/1.3422544

Cited by 83 publications

(90 citation statements)

References 9 publications

(7 reference statements)

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“…Recently, micro-orifice structure was reported to assure one-to-one cell pairs and follow-up fusion procedure. 23,24 A meniscus-based fabrication approach was adopted to create microorifice with a diameter of 2-10 lm on the vertical walls in a microfluidic channel. Only seven cells from each side of the wall were attracted to the microorifice to form a cell pair (95-100%) under a 1 MHz alternating current.…”

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confidence: 99%

A microfluidic chip for highly efficient cell capturing and pairing

et al. 2011

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This paper examined the feasibility of a microfluidics chip for cell capturing and pairing with a high efficiency. The chip was fabricated by the polydimethylsiloxanebased soft-lithography technique and contained two suction duct arrays set in parallel on both sides of a main microchannel. Cells were captured and paired by activating two sets of suction ducts one by one with the help of syringe pumps along with switching the cell suspensions inside the main microchannel correspondingly. The effects of suction flow rate and the dimensions of suction channels on the cell capturing and pairing efficiency were characterized. The present chip was capable of creating 1024 pairs of two different cell populations in parallel. The preliminary experimental results showed that the cell capturing efficiency was 100% and the pairing one was 88% with an optimal suction rate of 5 ll/min in the chip in the 2 lm-sized suction duct chip. The cell viability after capture inside the microfluidic device was 90.0 6 5.3%. With this cell capturing and pairing chip, interaction between cells in a single pair mode can be studied. The ability to create cell pairs has a number of biological applications for cell fusion, cell-cell interaction studies, and cell toxicity screening.

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confidence: 99%

A microfluidic chip for highly efficient cell capturing and pairing

et al. 2011

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“…13 Recently, microfluidics-based cell electrofusion is obtaining more and more attention due to its low voltage, low Joule-heating effect, and precise controllability. 11,[13][14][15][16][17][18][19][20][21][22][23][24][25][26] Due to the short distance between the patterned microelectrodes, a low voltage is sufficient to generate high electric field required for electrofusion. Therefore, it eliminates the requirement for the expensive high-voltage power generator.…”

Section: Introductionmentioning

confidence: 99%

“…The existing studies on microfluidics-based cell electrofusion mainly focused on improving cell pairing and fusion efficiencies. Typically, positive DEP under AC electric field is used to control cell pairing, [14][15][16][17][18][19][20][21] and DC pulses are used for reversible electroporation and electrofusion in the microfluidic devices. Compared with the conventional electrofusion methods, the microfluidic devices have advantages of low voltage and high efficiency in cell pairing.…”

Section: Introductionmentioning

confidence: 99%

“…13 However, this method requires modification of cells. Gel et al 18,19 recently developed a novel micro-orifice based cell electrofusion microfluidic device without using 3D microelectrodes. The micro-orifice, whose diameter is smaller than that of the cells, creates field constriction resulting in membrane breakdown occurring nowhere but in the micro-orifice.…”

Section: Introductionmentioning

confidence: 99%

“…However, it still uses parallel plate electrodes with a large distance to induce reversible electroporation and fusion and thus has inherent disadvantages of the convectional electrofusion method. In the existing studies, both planar thin-film microelectrode [18][19][20][21] and bulk microelectrode 11,13,14,16 have been widely used. The planar thin film microelectrodes are deposited at the bottom edge of the side walls of the microchannel and do not cover the entire side walls.…”

Section: Introductionmentioning

confidence: 99%

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A cell electrofusion microfluidic device integrated with 3D thin-film microelectrode arrays

Yang

Qian

et al. 2011

Biomicrofluidics

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A microfluidic device integrated with 3D thin film microelectrode arrays wrapped around serpentine-shaped microchannel walls has been designed, fabricated and tested for cell electrofusion. Each microelectrode array has 1015 discrete microelectrodes patterned on each side wall, and the adjacent microelectrodes are separated by coplanar dielectric channel wall. The device was tested to electrofuse K562 cells under a relatively low voltage. Under an AC electric field applied between the pair of the microelectrode arrays, cells are paired at the edge of each discrete microelectrode due to the induced positive dielectrophoresis. Subsequently, electric pulse signals are sequentially applied between the microelectrode arrays to induce electroporation and electrofusion. Compared to the design with thin film microelectrode arrays deposited at the bottom of the side walls, the 3D thin film microelectrode array could induce electroporation and electrofusion under a lower voltage. The staggered electrode arrays on opposing side walls induce inhomogeneous electric field distribution, which could avoid multi-cell fusion. The alignment and pairing efficiencies of K562 cells in this device were 99% and 70.7%, respectively. The electric pulse of low voltage ($9 V) could induce electrofusion of these cells, and the fusion efficiency was about 43.1% of total cells loaded into the device, which is much higher than that of the convectional and most existing microfluidics-based electrofusion devices.

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High‐Density Microfluidic Particle‐Cluster‐Array Device for Parallel and Dynamic Study of Interaction between Engineered Particles

Kim

Lee

et al. 2017

Advanced Materials

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A high-density and high-performance microfluidic particle-cluster-array device utilizing a novel hydrodynamically tunable pneumatic valve (HTPV) is reported for parallel and dynamic monitoring of the interactions taking place in particle clusters. The key concept involves passive operation of the HTPV through elastic deformation of a thin membrane using only the hydrodynamic force inherent in microchannel flows. This unique feature allows the discrete and high-density (≈30 HTPVs mm ) arrangement of numerous HTPVs in a microfluidic channel without any pneumatic connection. In addition, the HTPV achieves high-performance clustering (≈92%) of three different particles in an array format through the optimization of key design and operating parameters. Finally, a contamination-free, parallel, and dynamic biochemical analysis strategy is proposed, which employs a simple one-inlet-one-outlet device operated by the effective combination of several techniques, including particle clustering, the interactions between engineered particles, two-phase partitioning and dehydration control of aqueous plugs, and shape/color-based particle identification.

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Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array

Cited by 83 publications

References 9 publications

A microfluidic chip for highly efficient cell capturing and pairing

A microfluidic chip for highly efficient cell capturing and pairing

A cell electrofusion microfluidic device integrated with 3D thin-film microelectrode arrays

High‐Density Microfluidic Particle‐Cluster‐Array Device for Parallel and Dynamic Study of Interaction between Engineered Particles

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