Dielectrophoresis based cell switching in continuous flow microfluidic devices

Mathew, Bobby; Alazzam, Anas; Destgeer, Ghulam; Sung, Hyung Jin

doi:10.1016/j.elstat.2016.09.003

Cited by 34 publications

(20 citation statements)

References 26 publications

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“…In biomedical microfluidic devices, the ability to switch the trajectory of micro particles and cells to a desired location can be used to perform operations such as such as washing, functionalization and medium exchange. Previously DEP has been used to both focus and subsequently manipulate a stream of focused micro-particles to achieve cell washing [66]. For example, Tarn et al [67] showed how force can be used to manipulate the trajectory of micro-particles into different liquid streams within a microchannel, and thereby consecutively pass particles through two reagents and a washing solution.…”

Section: Rbc Trajectory Switchingmentioning

confidence: 99%

Versatile microfluidic platform embedded with sidewall three-dimensional electrodes for cell manipulation

Puttaswamy¹,

Fishlock²,

Steele³

et al. 2019

Biomed. Phys. Eng. Express

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The trapping and manipulation of single and small numbers of cells is becoming increasingly important for the development and understanding of cell biology, disease predication and disease diagnostics. In the present work, we developed two dielectrophoresis (DEP) based microfluidic devices, both embedded with three-dimensional (3D) microelectrodes. The first microfluidic device is used for the trajectory switching of cells. The second is a single microfluidic platform used for cell concentration, trapping of single, two cells (doublets) and three cell clusters (triplet). Red blood cell (RBC) trajectory switching to different outlets was achieved by applying 20 Vpp at 1kHz to the 3D microelectrodes. RBC pre-concentration and trapping was realized by applying 10 Vpp at 5 MHz. During RBC trapping at 5 % hematocrit, a trapping efficiency of up to 84 % was achieved for doublets and triplets, and at 1 % hematocrit, a 67 % single cell trapping efficiency was obtained. RBC trajectory switching takes place in ~2 to 4 seconds and cell trapping in ~8 to 10 seconds following the application of electric field. We performed simulations on comparable 2D planar and 3D microelectrodes which confirmed that 3D microelectrodes support more uniform particle manipulation throughout the channel height direction.

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Section: Rbc Trajectory Switchingmentioning

confidence: 99%

Versatile microfluidic platform embedded with sidewall three-dimensional electrodes for cell manipulation

Puttaswamy¹,

Fishlock²,

Steele³

et al. 2019

Biomed. Phys. Eng. Express

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show abstract

“…Piezoelectric transducers have effectively been used to generate acoustic forces for medium exchange of polystyrene (PS) particles [ 6 ]. Other techniques for manipulation of single cells include optical [ 7 ], electric [ 8 ], and mechanical [ 9 ] methods. Among them, the electrical-based technique, called dielectrophoresis (DEP) [ 10 ], to perform medium exchange and cell dipping, is introduced in this work.…”

Section: Introductionmentioning

confidence: 99%

Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis

et al. 2018

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Medium exchange is the process of changing the suspension medium of cells/particles, and has applications in washing, surface modifications, nutrient replenishment, or simply changing the environment of the target entities. Dipping involves diverting the path of target cells in the carrying fluid to immerse them in another fluid for a short duration, and pushing them again into the original medium. In this paper, a simple microfluidic platform is introduced that employs dielectrophoresis to achieve medium exchange and dipping of micro-objects in a continuous manner. The essential feature of the platform is a microchannel that includes two arrays of microelectrodes that partly enter the bottom surface from both sides. In the first step, numerous finite element-based parametric studies are carried out to obtain the optimized geometrical and operational parameters ensuring successful dipping and medium exchange processes. The results of those studies are utilized to fabricate the platform using standard photolithography techniques. The electrodes are patterned on a glass substrate, while the channel, made out of polydimethylsiloxane, is bonded on top of the glass. Trajectories of blood cells from numerical studies and experimentations are reported, and both results exhibited close agreement.

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“…In this work, a DEP‐based microfluidic device for separation of cells is developed. DEP is a noninvasive separation method with slight or no aggression to the biophysical integrity of cells . DEP as a phenomenon is the motion of neutral, polarizable particles under the effect of nonuniform electric field .…”

Section: Introductionmentioning

confidence: 99%

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

2017

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We describe the design, microfabrication, and testing of a microfluidic device for the separation of cancer cells based on dielectrophoresis. Cancer cells, specifically green fluorescent protein-labeled MDA-MB-231, are successfully separated from a heterogeneous mixture of the same and normal blood cells. MDA-MB-231 cancer cells are separated with an accuracy that enables precise detection and counting of circulating tumor cells present among normal blood cells. The separation is performed using a set of planar interdigitated transducer electrodes that are deposited on the surface of a glass wafer and slightly protrude into the separation microchannel at one side. The device includes two parts, namely, a glass wafer and polydimethylsiloxane element. The device is fabricated using standard microfabrication techniques. All experiments are conducted with low conductivity sucrose-dextrose isotonic medium. The variation in response between MDA-MB-231 cancer cells and normal cells to a certain band of alternating-current frequencies is used for continuous separation of cells. The fabrication of the microfluidic device, preparation of cells and medium, and flow conditions are detailed. The proposed microdevice can be used to detect and separate malignant cells from heterogeneous mixture of cells for the purpose of early screening for cancer.

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Dielectrophoresis based cell switching in continuous flow microfluidic devices

Cited by 34 publications

References 26 publications

Versatile microfluidic platform embedded with sidewall three-dimensional electrodes for cell manipulation

Versatile microfluidic platform embedded with sidewall three-dimensional electrodes for cell manipulation

Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

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