Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs

Kumar, Narendra; Wang, Wenjian; Ortiz‐Marquez, Juan C.; Catalano, Matthew; Gray, Mason; Biglari, Nadia; Kitadai, Hikari; Ling, Xi; Gao, Jianmin; Opijnen, Tim van; Burch, Kenneth S.

doi:10.1016/j.bios.2020.112123

Cited by 70 publications

(59 citation statements)

References 52 publications

(2 reference statements)

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“…Further, to prevent false positives that could occur due to nonspecific adsorption of CA1, it is crucial to obtain uniform functionalization of aptamers on the graphene surface. As such, the attachment process was optimized using atomic force microscopy (AFM) to determine attachment and coverage of the aptamers (Kumar et al, 2020). Patterned graphene chips were incubated for 30 minutes with high concentration (10 m M ) PBASE linker dissolved in DMF (Wang et al, 2015; Xu et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Graphene Field Effect Transistors (G‐FETs) have shown great promise in the detection of DNA, proteins and cells due to their high sensitivity, scalability, biocompatibility, ease of functionalization via a non‐covalent attachment process and compatibility with various substrates (Fu, et al, 2017; Fu et al, 2017; Gao et al, 2016; Kumar et al, 2020; Ohno et al, 2009, 2010; Ping et al, 2016; Wang et al, 2015; Xu et al, 2017). To determine the possibility of POC detection of CA, it is crucial to investigate the mechanisms of interaction between G‐FETs and CA1, their detection limit, sensitivity, selectivity and utility in a clinically relevant environment (Kostarelos & Novoselov, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…To achieve high sensitivity, we produced clean and reproducible G‐FETs by fabricating them in our cleanroom in a glovebox using the same process that produced devices for selective detection of antibiotic‐resistant bacteria (Kumar et al, 2020). Our facility allows rapid prototyping with minimal reduction in graphene quality from unwanted impurities that subsequently reduce the accuracy and sensitivity (Macedo et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Detection of a multi‐disease biomarker in saliva with graphene field effect transistors

et al. 2020

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of a multi‐disease biomarker in saliva with graphene field effect transistors

et al. 2020

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“…28,30,34,35,36,37 In our recent work, we demonstrated a highly sensitive G-FET for the detection of biomarkers such as CA-I (oral diseases biomarker) in saliva, 38 and antibiotic resistant bacteria, both at clinically relevant concentrations. 39 However, the G-FET design in our prior work was limited to detection of a single target, with each chip functionalized with a single probe, provided minimal passivation, and required a platinum (Pt) wire as a separate reference electrode.…”

Section: Introductionmentioning

confidence: 99%

Rapid, multianalyte detection of opioid metabolites in wastewater

Kumar

Rana

Geiwitz

et al. 2021

Preprint

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By monitoring opioid metabolites, wastewater-based epidemiology (WBE) could be an excellent tool for real-time information on consumption of illicit drugs. A key limitation of WBE is the reliance on costly laboratory-based techniques that require substantial infrastructure and trained personnel, resulting in long turnaround times. Here, we present an aptamer-based graphene field effect transistor (AptG-FET) platform for simultaneous detection of three different opioid metabolites. This platform provides a reliable, rapid, and inexpensive method for quantitative analysis of opioid metabolites in wastewater (WW). The platform delivers a limit of detection (LOD) 2-3 orders of magnitude lower than previous reports, but in line with the concentrations range (pg/ml to ng/ml) of these opioid metabolites present in real samples. To enable multianalyte detection we developed a facile, reproducible, and high yield fabrication process producing twenty G-FETs with integrated side gate platinum (Pt) electrodes on a single chip. Our devices achieved the simultaneous and selective multianalyte detection of three different metabolites: Noroxycodone (NX), 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and Norfentanyl (NF) in wastewater.

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“…In our previous work, we utilized the DEP technique to place SrTiO 3 nanoparticles between two electrodes and measure their electrical properties [17]. In the biological science field, DEP has also been utilized for trapping microorganisms [18,19], cell separation and characterization [1,[20][21][22][23], preventing membrane fouling in bioreactors [24], and bacterial isolation [25]. Even though the technique is useful, and many studies have been reported, the conventional DEP technique has a major constraint: it requires attaching wires directly to the electrodes, which may limit some applications.…”

Section: Introductionmentioning

confidence: 99%

Wirelessly powered dielectrophoresis of metal oxide particles using spark‐gap Tesla coil

et al. 2020

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Wirelessly powered dielectrophoresis (DEP) of metal oxide particles was performed using a spark‐gap Tesla coil (TC). The main contribution of this work is the simplification of the conventional DEP setup that requires attaching wires directly to the electrodes. Wireless power from the TC generates a high output frequency and voltage, which corresponds to that used for the DEP. Therefore, a spark‐gap TC was built and utilized to conduct the DEP process. Metal oxides (ZnO and Fe2O3) were used as targets for the assembly. The results showed that the wirelessly powered DEP technique via a TC was successful in assembling the metal oxide particles. Positive and negative DEP phenomena were observed. Positive DEP occurred during ZnO assembly, making particles chain grow 0.92 mm toward the sparks within 60 s. Negative DEP was observed during Fe2O3 assembly, where the repulsion of particles formed a void around the sparks with a 1.45 mm radius. The mechanism of this wireless DEP system is discussed.

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Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs

Cited by 70 publications

References 52 publications

Detection of a multi‐disease biomarker in saliva with graphene field effect transistors

Detection of a multi‐disease biomarker in saliva with graphene field effect transistors

Rapid, multianalyte detection of opioid metabolites in wastewater

Wirelessly powered dielectrophoresis of metal oxide particles using spark‐gap Tesla coil

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