Dielectric spectroscopy of Tobacco Mosaic Virus

Ermolina, I.; Morgan, Hywel; Green, Nicolas G.; Milner, Joel J.; Feldman, Yuri

doi:10.1016/s0304-4165(03)00118-1

Cited by 44 publications

(36 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Image analysis of 100 virus particles revealed an average size for TMV of 280nm in length and 17nm in width (Fig 2). The ultrastructure in TMV-infected cells and the size of virions observed in this study were found to be in accordance with TMV-induced ultrastructural properties in tobacco and the size range of TMV particles previously reported in the literature [9][10][11][12][13][14][15] . …”

Section: Representative Resultssupporting

confidence: 91%

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Zechmann

Graggaber

Zellnig

2011

JoVE

View full text Add to dashboard Cite

Investigations of ultrastructural changes induced by viruses are often necessary to clearly identify viral diseases in plants. With conventional sample preparation for transmission electron microscopy (TEM) such investigations can take several days 1,2 and are therefore not suited for a rapid diagnosis of plant virus diseases. Microwave fixation can be used to drastically reduce sample preparation time for TEM investigations with similar ultrastructural results as observed after conventionally sample preparation [3][4][5]

show abstract

Section: Representative Resultssupporting

confidence: 91%

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Zechmann

Graggaber

Zellnig

2011

JoVE

View full text Add to dashboard Cite

show abstract

“…These studies provide the ability to trap and manipulate nanoparticles with a real-time detection method desirable in molecular analysis, virology, clinical, and environmental fields. Concerning environmental applications, Morgan and coworkers have extensively studied two nonenveloped viruses: cowpea mosaic virus (CPMV) [58] and tobacco mosaic virus (TMV) [59][60][61]. Previously, in the late 1990s, Morgan and Green used mathematical modeling to predict the dielectrophoretic response and crossover frequency of TMV, finding that at frequencies below the crossover TMV exhibited positive DEP, and above the crossover negative DEP [59].…”

Section: Virus Monitoringmentioning

confidence: 99%

Dielectrophoretic monitoring of microorganisms in environmental applications

Jesús‐Pérez¹,

Lapizco‐Encinas²

2011

Electrophoresis

View full text Add to dashboard Cite

Dielectrophoresis (DEP) is the motion of polarizable particles in the presence of nonuniform electric fields. This novel electrokinetic technique has successfully been employed in many miniaturized systems for the manipulation and detection of microbes. This review article depicts the application of dielectrophoresis for the monitoring of microorganisms in microfluidic devices for environmental applications. The research studies described here are mainly conceived for water- and air-monitoring assessments, and are classified considering the target aimed to detect, concentrate, and/or separate, including chemical and toxicant agents, and microorganisms ranging from virus to protozoa. Dielectrophoresis has also played an important role in biofilm formation studies. This review article comprises mainly studies published from 2000 to present. Even in this relatively short time frame, there have been many significant contributions of this powerful and nascent technique related to environmental monitoring; thus, unveiling its great potential for future research directions.

show abstract

“…Assuming that the protein undergoes a reversible two-state transition from native ( ) to unfolded ( ) states, the equilibrium constant can be defined as (1) Here, is the fraction of protein in the native state, is the gas constant, is the temperature (in degrees Kelvin), and is the midpoint temperature (defined as the temperature at which is equal to 0.50 and is equal to 1). The signal ( is any observable, in this case, either the UV/VIS absorbance or the peak frequency) was equal to (2) where and are, respectively, the native and unfolded baselines, defined as linear functions of temperature. An alternative baseline-free approach [28] was also used in which…”

Section: Spectroscopy and Thermal Denaturationmentioning

confidence: 99%

“…The technique has been successfully applied to biological specimens using various methods of application including time-domain reflectometry [2], [3], transmission lines [4], coaxial probes [5], and microfabricated waveguides [6]. While these studies have yielded potentially useful results, many of them suffer from one or more of the following disadvantages.…”

mentioning

confidence: 99%

Ultra-sensitive detection of protein thermal unfolding and refolding using near-zone microwaves

Taylor

Weide

2005

IEEE Trans. Microwave Theory Techn.

View full text Add to dashboard Cite

Abstract-We use planar slot antennas proximal to proteins in solution to detect changes in conformation. The antennas are attached to fused-quartz or glass sample holders and the cuvette/antenna assembly is placed in the sample holder of an optical spectrophotomer (either UV/VIS or fluorescence polarization), allowing simultaneous dielectric and optical measurements. Return loss is recorded using a vector network analyzer. This system was used to study the equilibrium thermal unfolding and refolding of a small globular protein, as well as the binding of small hormones to a receptor. Good agreement between optical and microwave measurements was obtained for all systems studied. We show that microwave measurements can be made at protein concentrations as low as 0.3 ng/mL (19 pM), several orders of magnitude lower than that required for optical spectroscopy. The results from these experiments demonstrate that resonant slot antennas can be used to detect changes in protein conformation and the presence of microwave radiation does not perturb the system under study.Index Terms-Biomedical applications of electromagnetic radiation, biophysics, proteins, slot antennas.

show abstract

Dielectric spectroscopy of Tobacco Mosaic Virus

Cited by 44 publications

References 35 publications

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Dielectrophoretic monitoring of microorganisms in environmental applications

Ultra-sensitive detection of protein thermal unfolding and refolding using near-zone microwaves

Contact Info

Product

Resources

About