Dielectric spectroscopy of mammalian cells

Cerckel, I.; Garcia, Amaya; Degouys, V.; Dubois, Dominique; Fabry, L.; Miller, A. O. A.

doi:10.1007/bf00749814

Cited by 28 publications

(4 citation statements)

References 13 publications

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“…Some authors therefore proposed to make use of an adapting frequency which is dependent on several cell-specific factors and can change during the course of a fermentation. In brief, this so-called critical frequency fc shall explain the deviations in a mechanistic way by pricing-in influences ranging from cell size change, conductivity and others [ 7 , 31 , 32 ], thus extending the regular linear model by several terms and parameters for which a mathematical fit in a new model (Cole-Cole) is required. Although the concept is without a doubt plausible, all fitted coefficients require calculation, pre-smoothing steps of the data and might differ under different process conditions, reactor geometries, physico-chemical conditions and so on.…”

Section: Resultsmentioning

confidence: 99%

Universal Capacitance Model for Real-Time Biomass in Cell Culture

Konakovsky

Yagtu

Clemens

et al. 2015

Sensors

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Capacitance probes have the potential to revolutionize bioprocess control due to their safe and robust use and ability to detect even the smallest capacitors in the form of biological cells. Several techniques have evolved to model biomass statistically, however, there are problems with model transfer between cell lines and process conditions. Errors of transferred models in the declining phase of the culture range for linear models around +100% or worse, causing unnecessary delays with test runs during bioprocess development. The goal of this work was to develop one single universal model which can be adapted by considering a potentially mechanistic factor to estimate biomass in yet untested clones and scales. The novelty of this work is a methodology to select sensitive frequencies to build a statistical model which can be shared among fermentations with an error between 9% and 38% (mean error around 20%) for the whole process, including the declining phase. A simple linear factor was found to be responsible for the transferability of biomass models between cell lines, indicating a link to their phenotype or physiology.

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Section: Resultsmentioning

confidence: 99%

Universal Capacitance Model for Real-Time Biomass in Cell Culture

Konakovsky

Yagtu

Clemens

et al. 2015

Sensors

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“…is measured with a biomass sensor placed inside the fixed-bed (in ) (Cerckel et al 1993;Fogale nanotech 2010). The size of the biomass sensor does not enable to use it in the bioreactor FBB 2 .…”

Section: Bioreactors and Cell Culturesmentioning

confidence: 99%

Segregated Model of Adherent Cell Culture in a Fixed-Bed Bioreactor

Gelbgras¹,

Wylock²,

Drugmand³

et al. 2011

Chemical Product and Process Modeling

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In this work, a mathematical model of a fixed-bed bioreactor for the animal cell culture is developed to study the optimization and the scale-up of this bioreactor. Several cell populations are considered: the cells in suspension in the medium at the beginning of the process and the adhering cells to the fixed-bed. The model includes a capture rate kinetic of the cells in suspension by the fixed-bed and a spatial distribution of the nutrient and by-product concentrations in the fixed-bed. Therefore, the model reports the potential gradients of the cell concentrations in the fixed-bed. Some model parameters are experimentally identified and the model is validated using experimental data obtained with two pilot bioreactors. The model is used as a simulation tool to study the influence of the bioreactor design or the velocity field of the culture medium on the cell concentration gradients in the fixed-bed bioreactor and to optimize the operating conditions, the design, and the scale-up of this bioreactor.

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“…The time evolutions of the flow rate of the feeding medium for the different cultures are presented in Figure 6. The cell concentration is measured with a biomass sensor placed at the top of the fixed-bed (Cerckel et al 1993) or on a sample of BioNOCII TM by the crystal violet method (Table 8) (Freshney 2005). The concentrations of glucose, glutamine, glutamate, lactate and ammonia in the medium can be measured in the pump by a BioProfile analyser (Nova Biomedical 2010).…”

Section: Animal Cell Culture In Fixed-bed Bioreactormentioning

confidence: 99%

Structured Model of VERO Cells Metabolism in a Fixed-Bed Bioreactor

Gelbgras¹,

Wylock²,

Drugmand³

et al. 2011

Chemical Product and Process Modeling

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In this work, a structured mathematical model of the VERvet Origin (VERO) cell metabolism in a fixed-bed bioreactor is developed. Mass balance equations for the extra-and intracellular species are written considering the main pathways of the animal cell metabolism. From the model equations, an undetermined set of equations relating the consumption or production velocities of the extracellular species and the velocities of the metabolic reactions can be developed. The monitoring of a reference cell culture enables to transform this undetermined set of equations in a determined one. The resolution of this determined set of equations enables to analyze and characterize the cell metabolism. From the model and the cell metabolism characterization, a monitoring tool is developed to simulate the time evolution of the species concentrations when the time evolution of the cell concentration is known. This simulation tool is used to simulate five experimental cell cultures. The comparison of the experimentally determined and computed time evolutions of the species concentrations enables to validate the developed monitoring tool. The validated monitoring tool can be used to optimize the operating conditions of the culture, as the medium feeding rate, the range of the species concentrations.

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Dielectric spectroscopy of mammalian cells

Cited by 28 publications

References 13 publications

Universal Capacitance Model for Real-Time Biomass in Cell Culture

Universal Capacitance Model for Real-Time Biomass in Cell Culture

Segregated Model of Adherent Cell Culture in a Fixed-Bed Bioreactor

Structured Model of VERO Cells Metabolism in a Fixed-Bed Bioreactor

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