Dielectric Properties of Water in Myoglobin Solution

Grant, E H; Nightingale, N R V; Sheppard, R J; Gough, S. R.

doi:10.1021/bk-1981-0157.ch003

Cited by 6 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16,19,87 Protein reorientation occurs on a nanosecond time scale. 88 We can see in these results that over 97% of the equilibration is obtained within the time scales that are equivalent to interaction with bulk water, indicating that slower nanosecond protein reorientation does not contribute significantly.…”

Section: The Journal Of Physical Chemistry Lettersmentioning

confidence: 75%

Broad-Band Pump–Probe Spectroscopy Quantifies Ultrafast Solvation Dynamics of Proteins and Molecules

Jumper

Arpin

Turner

et al. 2016

J. Phys. Chem. Lett.

View full text Add to dashboard Cite

In this work, we demonstrate the use of broad-band pump-probe spectroscopy to measure femtosecond solvation dynamics. We report studies of a rhodamine dye in methanol and cryptophyte algae light-harvesting proteins in aqueous suspension. Broad-band impulsive excitation generates a vibrational wavepacket that oscillates on the excited-state potential energy surface, destructively interfering with itself at the minimum of the surface. This destructive interference gives rise to a node at a certain probe wavelength that varies with time. This reveals the Gibbs free-energy changes of the excited-state potential energy surface, which equates to the solvation time correlation function. This method captures the inertial solvent response of water (∼40 fs) and the bimodal inertial response of methanol (∼40 and ∼150 fs) and reveals how protein-buried chromophores are sensitive to the solvent dynamics inside and outside of the protein environment.

show abstract

Section: The Journal Of Physical Chemistry Lettersmentioning

confidence: 75%

Broad-Band Pump–Probe Spectroscopy Quantifies Ultrafast Solvation Dynamics of Proteins and Molecules

Jumper

Arpin

Turner

et al. 2016

J. Phys. Chem. Lett.

View full text Add to dashboard Cite

show abstract

“…A cell made of glass whose electrodes made of platinized platinum is used for the dielectric measurements. The value of cell constant (K) could be determined by using methanol solution to be 0.0265 m −1 which is kept constant during the process of curve fitting for the measured data of C and R. The setup of measuring electrode is made appropriate so as to minimize the impedance of the electrode polarization 6 , 13 , 14 . The residual of electrode polarization impedance is regarded in the iterations carried out to fit the experimental data.…”

Section: Methodsmentioning

confidence: 99%

“…However, studies toward the global structure of PM fragments may clarify previous unresolved behaviours rather than just studying the local fine structure. As a non-optical technique, the dielectric spectroscopy 6 in its time-independent measurements has enabled one to monitor reorientations in PM and to have an action spectrum of PM chromatic responses.…”

Section: Introductionmentioning

confidence: 99%

Action spectrum for reorientations in bacteriorhodopsin of purple membrane in suspension

Mostafa

2023

Sci Rep

View full text Add to dashboard Cite

In the present study, the dependency of purple membrane (PM) dielectric responses on the wavelength of light in the range 380–750 nm has showed meaningful changes about the rotation of PM in suspension and about the rotation of bacteriorhodopsin (bR) trimer inside PM, as well. The action spectrum of PM random walk substantiates the existence of two states of bR. One of them (blue edge-state) lies at the blue edge and the other (red edge-state) at the red edge of the visible absorption of bR. The results might bear on correlation of these bands to some bR photocycle intermediates or bR photoproducts. The results implicate the protein–chromophore interactions that eventually underlie protein–lipid interactions. Disrupting the protein–lipid contact during the illumination with light of wavelength in ranges of (410–470 nm) and (610–720 nm) has resulted in emergence of distinct dielectric dispersion at 0.06–0.08 MHz which is comparable to the size of bR trimer or monomer.The work reports on the chromatic adaptation of bR in view of the dielectric spectral parameters of PM. It aimed to explore a correlation seemingly found between the light wavelength and the relaxations of bR trimer inside PM. Changes in rotational diffusion of bR trimer upon blue and red light illumination can influence the three dimensional data storage based on bR, which may implicate bR in bioelectronics.

show abstract

Electromagnetic Energy: A Historical Therapeutic Perspective; its Future

Bentall¹

1990

Emerging Electromagnetic Medicine

View full text Add to dashboard Cite

Dielectric Properties of Water in Myoglobin Solution

Cited by 6 publications

References 0 publications

Broad-Band Pump–Probe Spectroscopy Quantifies Ultrafast Solvation Dynamics of Proteins and Molecules

Broad-Band Pump–Probe Spectroscopy Quantifies Ultrafast Solvation Dynamics of Proteins and Molecules

Action spectrum for reorientations in bacteriorhodopsin of purple membrane in suspension

Electromagnetic Energy: A Historical Therapeutic Perspective; its Future

Contact Info

Product

Resources

About