Dielectric Properties of Single Cells Subjected to Heat Shock Using DEP Cytometry

Afshar, Samaneh; Fazelkhah, Azita; Salimi, Elham; Butler, Michael; Thomson, D. J.; Bridges, Greg E.

doi:10.1109/tmtt.2018.2879350

Cited by 13 publications

(7 citation statements)

References 30 publications

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“…It means an increase of 1.6 × 10 −4 S/m approximately, which is closer to the value proposed in this paper than the one shown in [46]. In addition, other authors [56] explain the phenomenon of the decrease in cytoplasm conductivity because of a loss of ions. Such a loss is explained by the cell membrane leakage commented above.…”

Section: Discussionsupporting

confidence: 87%

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Thermal Shock Response of Yeast Cells Characterised by Dielectrophoresis Force Measurement

García-Diego

Rubio-Chavarría

Beltrán

et al. 2019

Sensors

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Dielectrophoresis is an electric force experienced by particles subjected to non-uniform electric fields. Recently, several technologies have been developed focused on the use of dielectrophoretic force (DEP) to manipulate and detect cells. On the other hand, there is no such great development in the field of DEP-based cell discrimination methods. Despite the demand for methods to differentiate biological cell states, most DEP developed methods have been focused on differentiation through geometric parameters. The novelty of the present work relies upon the point that a DEP force cell measurement is used as a discrimination method, capable of detecting heat killed yeast cells from the alive ones. Thermal treatment is used as an example of different biological state of cells. It comes from the fact that biological properties have their reflection in the electric properties of the particle, in this case a yeast cell. To demonstrate such capability of the method, 279 heat-killed cells were measured and compared with alive cells data from the literature. For each cell, six speeds were taken at different points in its trajectory inside a variable non-uniform electric field. The electric parameters in cell wall conductivity, cell membrane conductivity, cell membrane permittivity of the yeast cell from bibliography explains the DEP experimental force measured. Finally, alive and heat-treated cells were distinguished based on that measure. Our results can be explained through the well-known damage of cell structure characteristics of heat-killed cells.

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Section: Discussionsupporting

confidence: 87%

“…Such a loss is explained by the cell membrane leakage commented above. This layer behaviour has been studied not only for yeast cells but for Chinese hamster ovary cells too [56] in a temperature spectrum ranged from 7 °C to 50 °C. Surprisingly, the effect is inverted for low temperatures [57] and cell membrane conductivity is decreased.…”

Section: Discussionmentioning

confidence: 99%

Thermal Shock Response of Yeast Cells Characterised by Dielectrophoresis Force Measurement

García-Diego

Rubio-Chavarría

Beltrán

et al. 2019

Sensors

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“…Recently, methods capable of achieving cellular characterization from the qualitative measurement of the alteration of the cell’s mechanical properties have been developed. Conversely, there are few works focused on the measurement of the dielectric parameters that enable us to obtain the value of the exerted DEP over the cells [16,17,36,37,38]. These research articles, as well as this one, based their theoretical models on the mathematical expression of DEP given by Pohl [39].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Simple and Double Yeast Cells Using Dielectrophoretic Force Measurement

García-Diego

Rubio-Chavarría

Beltrán

et al. 2019

Sensors

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Dielectrophoretic force is an electric force experienced by particles subjected to non-uniform electric fields. In recent years, plenty of dielectrophoretic force (DEP) applications have been developed. Most of these works have been centered on particle positioning and manipulation. DEP particle characterization has been left in the background. Likewise, these characterizations have studied the electric properties of particles from a qualitative point of view. This article focuses on the quantitative measurement of cells’ dielectric force, specifically yeast cells. The measures are obtained as the results of a theoretical model and an instrumental method, both of which are developed and described in the present article, based on a dielectrophoretic chamber made of two V-shaped placed electrodes. In this study, 845 cells were measured. For each one, six speeds were taken at different points in its trajectory. Furthermore, the chamber design is repeatable, and this was the first time that measurements of dielectrophoretic force and cell velocity for double yeast cells were accomplished. To validate the results obtained in the present research, the results have been compared with the dielectric properties of yeast cells collected in the pre-existing literature.

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“…The error bars are associated with the impact of size variation on the estimated Re{ K CM }. The experimental estimate for the K CM and the previously measured Re{ K CM } are within 0.08 [12,62]. Being able to detect changes in K CM of 0.08 is useful as biological changes such as apoptosis produce a change of 0.4 in the K CM at 6 MHz [22].…”

Section: Resultsmentioning

confidence: 78%

“…5A, and these are shown in Fig. 5C along with the experimental estimate of the K CM for CHO cells [62]. The error bars are associated with the impact of size variation on the estimated Re{ K CM }.…”

Section: Resultsmentioning

confidence: 90%

Parallel single‐cell optical transit dielectrophoresis cytometer

et al. 2020

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In this work, we present an optical transit DEP flow cytometer for parallel single-cell analysis. Each cell's dielectric property is inferred from velocity perturbations due to DEP actuation in a microfluidic channel. Dual LED sources facilitate velocity measurement by producing two transit shadows for each cell passing through the channel. These shadows are detected using a 256-pixel linear optical array detector. Massively parallel analysis is possible as each pixel of the detector can independently analyze the passing cells. A wide channel (∼18 mm) was employed to carry many particles simultaneously, and the system was capable of detecting the velocity of over 200 cells simultaneously. We have achieved analysis rates for 10 µm diameter polystyrene spheres response exceeding 250 per second. With appropriate calibration, this DEP cytometer can quantitatively measure the dielectric response. The dielectric response (Clausius-Mossotti factor) of viable CHO cells was measured over the frequency range of 100 kHz to 6 MHz, and the obtained response matches the previously measured values by our group. The DEP cytometer uses simple modular components to achieve high throughput label-free single-cell dielectric analysis and can begin analyzing particles within 10 s after starting to pump the sample into the channel.

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Dielectric Properties of Single Cells Subjected to Heat Shock Using DEP Cytometry

Cited by 13 publications

References 30 publications

Thermal Shock Response of Yeast Cells Characterised by Dielectrophoresis Force Measurement

Thermal Shock Response of Yeast Cells Characterised by Dielectrophoresis Force Measurement

Characterization of Simple and Double Yeast Cells Using Dielectrophoretic Force Measurement

Parallel single‐cell optical transit dielectrophoresis cytometer

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