Dielectric measurement to monitor the growth and the physiological states of biological cells

Matanguihan, Ricaredo; Konstantinov, Konstantin; Yoshida, Takemi

doi:10.1007/bf00387695

Cited by 52 publications

(14 citation statements)

References 28 publications

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“…The relationship is rather governed by a mechanism in which dying cells loose their polarizability (and hence their permittivity) progressively. 21 Permittivity measurements do also reflect changes in cell physiology which has been demonstrated for a wide variety of cell types. 5,9,13,[22][23][24] Cell physiology is most commonly described by the term ''physiological state.''…”

Section: Introductionmentioning

confidence: 99%

“…Permittivity is a function of cell size, membrane integrity, and properties and also of changes in the cell's interior. 21 One can monitor cell size and follow the cell cycle progression and division of budding yeast by measuring the permittivity at very low frequencies. 27,28 Changes in cellular morphology, the capacitance per membrane area after viral infection 29,30 and viability 18 can also be monitored by permittivity measurements.…”

Section: Introductionmentioning

confidence: 99%

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Multifrequency permittivity measurements enable on‐line monitoring of changes in intracellular conductivity due to nutrient limitations during batch cultivations of CHO cells

Ansorge

Esteban

Schmid

2009

Biotechnology Progress

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Lab and pilot scale batch cultivations of a CHO K1/dhfr À host cell line were conducted to evaluate on-line multifrequency permittivity measurements as a process monitoring tool. The b-dispersion parameters such as the characteristic frequency (f C ) and the permittivity increment (De max ) were calculated on-line from the permittivity spectra. The dual-frequency permittivity signal correlated well with the off-line measured biovolume and the viable cell density. A significant drop in permittivity was monitored at the transition from exponential growth to a phase with reduced growth rate. Although not reflected in off-line biovolume measurements, this decrease coincided with a drop in OUR and was probably caused by the depletion of glutamine and a metabolic shift occurring at the same time. Sudden changes in cell density, cell size, viability, capacitance per membrane area (C M ), and effects caused by medium conductivity (r m ) could be excluded as reasons for the decrease in permittivity. After analysis of the process data, a drop in f C as a result of a fall in intracellular conductivity (r i ) was identified as responsible for the observed changes in the dual-frequency permittivity signal. It is hypothesized that the b-dispersion parameter f C is indicative of changes in nutrient availability that have an impact on intracellular conductivity r i . On-line permittivity measurements consequently not only reflect the biovolume but also the physiological state of mammalian cell cultures. These findings should pave the way for a better understanding of the intracellular state of cells and render permittivity measurements an important tool in process development and control.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multifrequency permittivity measurements enable on‐line monitoring of changes in intracellular conductivity due to nutrient limitations during batch cultivations of CHO cells

Ansorge

Esteban

Schmid

2009

Biotechnology Progress

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“…Another promising approach is the estimation of viable biomass in cell cultures based on the determination of capacitance or its normalized measure, radiofrequency dielectric permittivity (Junker et al, 1994;Kell et al, 1990;Konstantinov et al, 1994;Matanguihan et al, 1994;Siano, 1997). The relative permittivity (Ј) of cell cultures has generally been found to be proportional to the membraneenclosed volume-fraction of the suspension (Harris et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements

Zeiser

Bédard

Voyer

et al. 1999

Biotechnol. Bioeng.

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“…Conversely, permittivity data reflect the polarization of the cell membrane when submitted to low radio frequencies (0.1-20 MHz). Hence, the dielectric permittivity is directly proportional to the viable biomass concentration, since dead cells do not present polarization in their membranes (Matanguihan et al, 1994;Markx and Davey, 1999;Tibayrenc et al, 2011). Similarly, CO 2 production as well as O 2 uptake is also strongly influenced by the physiological state of the cells.…”

Section: On-line Estimation Of Cer Our Rq and μmentioning

confidence: 99%

A supervision and control tool based on artificial intelligence for high cell density cultivations

Horta

Silva

Sargo

et al. 2014

Braz. J. Chem. Eng.

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-High cell density cultivations of recombinant E. coli have been increasingly used for the production of heterologous proteins. However, it is a challenge to maintain these cultivations within the desired conditions, given that some variables such as dissolved oxygen concentration (DOC) and feed flow rate are difficult to control. This paper describes the software SUPERSYS_HCDC, a tool developed to supervise fed-batch cultures of rE. coli with biomass concentrations up to 150 g DCW /L and cell productivities up to 9 g DCW .L -1 .h -1 . The tool includes automatic control of the DOC by integrated action of the stirrer speed as well as of the air and oxygen flow rates; automatic start-up of the feed flow of fresh medium (system based on a neural network committee); and automatic slowdown of feeding when oxygen consumption exceeds the maximum capacity of the oxygen supply.

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Dielectric measurement to monitor the growth and the physiological states of biological cells

Cited by 52 publications

References 28 publications

Multifrequency permittivity measurements enable on‐line monitoring of changes in intracellular conductivity due to nutrient limitations during batch cultivations of CHO cells

Multifrequency permittivity measurements enable on‐line monitoring of changes in intracellular conductivity due to nutrient limitations during batch cultivations of CHO cells

On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements

A supervision and control tool based on artificial intelligence for high cell density cultivations

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