1972
DOI: 10.1098/rspa.1972.0083
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Dielectric dispersion and dipole moment of myoglobin in water

Abstract: The parameters of dielectric dispersion at radio frequencies in aqueous solutions of horse and sperm whale myoglobin have been measured as functions of protein concentration, solution conductivity and temperature. From these dependences it is shown that, of the likely interpretations, the mechanism of molecular rotation is best able to account for the observed dispersion. The results are consistent with a dipole moment of around 150D for the myoglobin molecule and a hydration shell of about two water molecules… Show more

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Cited by 86 publications
(18 citation statements)
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“…Consider now the significance of relaxation peaks in TSC of hydrated proteins. Among the polarization modes are (1) polarization of the protein molecule or parts of it, due to dipolar interactions with the applied field, an effect observed for instance with myoglobin (South & Grant, 1972), apatites (Hitmi et al 1986) and collagen (Lamure et al 1985) and (2) polarizations due to molecules of water surrounding the protein as shown by Jacobson (1955) with a possible distinction between molecules of water having different degrees of binding. The comparative TSC study of samples with different levels of hydration should allow discrimination between both polarization -100…”
Section: Thermally Stinudated Currents Measurementsmentioning
confidence: 95%
“…Consider now the significance of relaxation peaks in TSC of hydrated proteins. Among the polarization modes are (1) polarization of the protein molecule or parts of it, due to dipolar interactions with the applied field, an effect observed for instance with myoglobin (South & Grant, 1972), apatites (Hitmi et al 1986) and collagen (Lamure et al 1985) and (2) polarizations due to molecules of water surrounding the protein as shown by Jacobson (1955) with a possible distinction between molecules of water having different degrees of binding. The comparative TSC study of samples with different levels of hydration should allow discrimination between both polarization -100…”
Section: Thermally Stinudated Currents Measurementsmentioning
confidence: 95%
“…This comparative lack of asymmetry means that the dielectric dispersion due to the myoglobin molecule should be able to be represented to a good approximation by a single relaxation time, ie, by the Debye dispersion relationship. Such was shown by South and Grant [1972], who made extensive measurements of permittivity and conductivity of myoglobin solution in the frequency range 0.2-10 MHz, at seven concentrations and over a wide range of temperature, pH, and ionic strength. In addition to characterizing the main myoglobin dispersion, this work also established the existence of an additional small Debye dispersion at frequencies a little higher than 10 MHz.…”
mentioning
confidence: 91%
“…The description of the polar response in terms of the molecular charge distribution is more accurate for these solutes [7]. Given the length-scale of the external field variation in a typical dielectric or light-absorption experiment, the overall charge and the dipole moment are the two main multipoles to consider [8].…”
Section: Introductionmentioning
confidence: 99%