An experimental investigation on active flow control by a ns-DBD plasma actuator on a laminar shear layer generated at the verge of a backward facing step is reported. Reynolds number based on the step height was about 3600. Time resolved particle image velocimetry (tr-PIV) was employed at an acquisition frequency of 2kHz. The goal of this work was to gain insight about the frequency effect that determines the control authority reported in literature for this kind of plasma actuator. Therefore, frequency was the only parameter investigated, and the forcing frequency ranged between 20 and 200Hz. Ensemble averaged vector field results shown that the reattachment length after the step decreased by increasing the actuation frequency, reaching a minimum at a frequency of 160Hz. Above this frequency, up to 200Hz, the reattachment length increased again albeit remaining shorter than the non-actuated case. Fast Fourier Transform (FFT) analysis and Linear Stability Theory (LST) revealed a change into the stability of the controlled flow with respect to the stability of the base flow cause by the actuation. Proper Orthogonal Decomposition (POD) shown an effect of energy redistribution among modes induced by ns-DBD plasma actuator indicating a change of flow structures dominating the flow evolution, i.e. affecting laminar to turbulent transition. Because of viscous-inviscid flow interactions, according to the forcing frequency, different modes determine different flow structures that dominate the flow evolution, thus changing the overall stability of the controlled flow. Such phenomenon ultimately affects efficiency of transition from laminar to turbulent i.e. affecting the flow control authority of ns-DBDs, explaining the frequency effect reported in literature.