Cooking-related changes in residues of [14C]sulfamethazine (SMZ) and its metabolites were measured in pork products obtained from an animal given a single dose of the drug. In addition, the gastrointestinal absorption of residues after ingestion of cooked pork by dogs was assessed by analysis of blood and urine for SMZ and its metabolites. No loss of SMZ could be detected in pork loin, liver, and cured ham as a result of cooking by various methods. Levels of the IV4-acetyl metabolite of SMZ were not altered by cooking but the Ñ4-glucoside metabolite exhibited cooking-related declines of up to 70%. Liver, which contained high concentrations of the glucoside, exhibited an increase in SMZ as a result of cooking, and this could be explained by heat-related hydrolysis of SMZ-glucoside. Analysis of dog serum indicated slow but complete absorption of SMZ present in meat. Urine analysis showed the N-acetyl metabolite was also absorbed. The results indicate that cooking does not destroy and may increase SMZ in pork products and that ingestion of cooked pork can result in the absorption of drug and metabolite residues.