Diel, seasonal and vertical changes in the pelagic amphipod communities in the subarctic Pacific: insights from imaging analysis

Abstract

Vertically stratified day and night samples were collected from a depth of up to 1000 m during four seasons at a single station in the western subarctic Pacific. Subsequently, the abundance, biovolume, community structure and population structure of the pelagic amphipods were evaluated from the imaging analysis using ZooScan. The stable isotope ratio (δ15N) was also measured for each species. In total 10 amphipod species were identified in total, and their community structures were separated into four groups. The four communities were characterized by the upper-layer community dominated by Themisto pacifica, and the deep-water community that was characterized by the absence of T. pacifica. The other two groups were observed for the intermediate depths at night only, which was characterized by the low abundance of T. pacifica performing diel vertical migration from those depths. The deep-dwelling species displayed higher δ15N and positive relationships were detected with the habitat depth of each species. For the predominant T. pacifica, population structure (cohort) analysis was possible based on equivalent spherical diameter data from ZooScan. This study demonstrates that imaging analysis by ZooScan is applicable for analyses of minor taxonomic groups (amphipods) in zooplankton communities.