Die wachstumsfördernde Wirkung von Lipiden in Kulturen von Reiterspirochäten

Roemer, G. B.; Baltzersen, Elisabeth

doi:10.1007/bf02153167

Cited by 1 publication

(1 citation statement)

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“…The requirement by leptospires for free fatty acids is satisfied by enzymes present in the organisms which hydrolyze triglycerides and phospholipids (19,20). Members in the genus Treponema may be able to cleave fatty acids from monoglycerides (33), but evidence of phospholipid catabolism is lacking. Borreliae hydrolyze fatty acids from lysolecithin but cannot metabolize other major phospholipids or triglycerides.…”

Section: Discussionmentioning

confidence: 99%

Lipid Catabolism of Relapsing Fever Borreliae

Pickett

Kelly

1974

Infect Immun

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Relapsing fever borreliae require lipid compounds for growth in vitro. In this study, the major pathways of lipid catabolism in three species of tick-borne relapsing fever borreliae were investigated. Thin-layer chromatography was used to compare chloroform-methanol extracts of fresh culture media with extracts of exhausted culture media after organisms were removed by centrifugation. The chromatographic data demonstrated that lysolecithin was removed from the culture media during growth of the spirochetes, whereas lecithin, sphingomyelin, triglycerides, and cholesterol esters were not affected by growth of the organisms. Sonic extracts of the organism were tested for the presence of specific enzymes of lipid catabolism. Lysolecithinase, glycerophosphorylcholine diesterase, and acid phosphatase activities were demonstrated. Thus, these organisms can sequentially dissimilate lysolecithin to fatty acids, choline, inorganic phosphate, and glycerol. Assays for phospholipases A, C, and D, a-glycerophosphate dehydrogenase, alkaline phosphatase, and lipase were negative.

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Section: Discussionmentioning

confidence: 99%