Die Qualität diagnostischer Proben

Guder, W. G.; Ehret, W.; Fonseca-Wollheim, F. da; Heil, W.; Müller‐Plathe, O.; Schmitt, Y.; Töpfer, G.; Wisser, H.; Zawta, B.

doi:10.1046/j.1439-0477.2002.02036.x

Cited by 9 publications

(15 citation statements)

References 5 publications

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“…Anti-coagulants, thus, exert preservative action. This is nowhere explicitly reported, however, it is known that EDTA acts as powerful antioxidant and Guder et al (2002) state that anticoagulants have the purpose of preventing clotting and also ensuring maintenance of the original state. Wardrop (1995) also points out that anticoagulants are effective in preventing changes associated with storage.…”

Section: Resultsmentioning

confidence: 99%

Pre-Analytic Sources of Error with the Serum PAG-Pregnancy Test for Cows

Stahmann¹,

Gauly²,

Borstel³

et al. 2015

JAS

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The pregnancy associated glycoprotein (PAG) test for pregnancy detection in cows necessitates transportation of blood samples to the laboratory. This investigation addresses preanalytic sources of error that might compromise its reliability. During shipping blood samples undergo substantial temperature fluctuations (Experiment 1). Temperatures of whole blood beyond 0°C had no effect, whereas freezing reduced measurements by 22% at -10 °C and by 25% at -20 °C (Experiment 2). Freezing of blood with low PAG content (Experiment 3) caused an increase from 2.4 to 3.7 ng/ml (P < 0.01). Cryopreservation of serum with various PAG concentrations (Experiment 4) brought about increases to varying degrees. The presence of heparin and EDTA in collecting tubes had no effect on PAG measurements, whereas citrate caused an initial reduction, but remained stable thereafter (Experiment 5). In blood stored six months at chilling temperature no change in PAG values occurred as long as samples contained heparin or EDTA (Experiment 6). In Experiment 7 vortexing of whole blood showed no effect, whereas freezing and dilution with water seriously compromised results. In summary, to obtain reliable PAG measurements, contamination with water must be avoided; freezing of whole blood or serum and the use of collecting tubes containing citrate will result in inaccuracies without altogether distorting results. High ambient temperature, physical agitation and long term storage at chilling temperature in the presence of heparin or EDTA will have no impact. PAG determination in blood may thus be considered a reliable pregnancy test for cows in most situations.

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Section: Resultsmentioning

confidence: 99%

Pre-Analytic Sources of Error with the Serum PAG-Pregnancy Test for Cows

Stahmann¹,

Gauly²,

Borstel³

et al. 2015

JAS

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show abstract

“…Ein Transport auf Eis oder die Aufbewahrung im Kühlschrank können zu einer Kälteaktivierung von Gerinnungsfaktoren und damit zu einer Verkürzung der Gerinnungszeiten führen.Wenn eine zeitnahe Analyse nicht gewährleistet werden kann, sollte thrombozytenfreies Zitratplasma gewonnen und bei -20°C bzw. -70°C eingefroren werden [4,5,15].…”

Section: Transport Und Aufbewahrung Der Blutprobenunclassified

“…7; [15]). Im Vergleich zu Serum hat die Verwendung von Plasma aber den Vorteil, dass der Gerinnungsprozess nicht abgewartet werden muss.Plasmaproben können somit unmittelbar nach der Probennahme zentrifugiert werden.…”

Section: Serum = Vollblut Nach Abschluss Des Gerinnungsprozessesunclassified

“…So beeinträchtig freies Hämoglobin die Messung der Absorptionsphotometrie,die Pseudoperoxidaseaktivität des freien Hämoglobins interferiert mit verschiedenen Methoden der Bilirubinbestimmung und die von Blutzellen freigesetzten Proteasen beeinträchtigen die Aktivität von Gerinnungsfaktoren und damit die hämostaseo-logischen Laboranalysen. Zudem können aus Erythrozyten freigesetzte Adenylatkinasen die CK-und CK-MB-Bestimmung beeinflussen und zu falsch hohen CK-Werten führen [15].…”

Section: In-vivo-hämolyseunclassified

“…Daher sollte zur Vermeidung einer Lipoproteininterferenz unbedingt eine 12-stündige Nahrungskarenz bzw.nach parenteraler Gabe von Fettemulsionen ein 8-stündiger Abstand bis zur Probenentnahme eingehalten werden [10,15].…”

Section: In-vivo-hämolyseunclassified

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Der ?fehlerhafte? Laborbefund

Fiedler

Thiery²

2004

Der Internist

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Laboratory results play a key role in the diagnostic procedure, the decision of treatment and the follow up of diseases. A high validity of the laboratory result is an important precondition for the efficacy in clinical medicine. Analytical standards have been developed under strong quality control criteria, however, there are no sufficiently defined standards for the pre- and postanalytical phase in laboratory diagnostics. Thus, most of laboratory errors are caused by pre- and postanalytical mistakes. The competent knowledge of possible sources for laboratory errors is a critical precondition for their avoidance. Diagnostic sensitivity and specificity play an important role for the choice of a laboratory test, whereas the predictive value should be considered for the medical relevance of the test result. In addition, many interference factors, which may influence the results of the laboratory tests have to be considered as age, sex, race, lifestyle, drugs, pregnancy, specimen collection, quality and handling as well as special factors, which may influence the complex of immunological and molecular diagnostics.

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Aussage und Nutzen von Laborergebnissen

Bertsch¹,

Wisser²

2009

Das Laborbuch

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Die Qualität diagnostischer Proben

Cited by 9 publications

References 5 publications

Pre-Analytic Sources of Error with the Serum PAG-Pregnancy Test for Cows

Pre-Analytic Sources of Error with the Serum PAG-Pregnancy Test for Cows

Der ?fehlerhafte? Laborbefund

Aussage und Nutzen von Laborergebnissen

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