Die papierchromatographische Trennung und Bestimmung der Uronsäuren

Fischer, Franz; Dörfel, Helmut

doi:10.1515/bchm2.1955.301.1-2.224

Cited by 132 publications

(6 citation statements)

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“…Descending chromatography was performed in: (a) 1-butanol-ethanol-water (5:1:4) (Partridge, 1948), (b) 1-butanol-pyridine-water (6:4:3) (French et al, 1950), and (c) these reagents in the proportion 10:3:3 (Hough et al, 1950); (d) 90% aqueous phenol (v/w) (Partridge, 1948) containing 0.08% 8-hydroxyquinoline in an atmosphere saturated with NHS and NaCN (Block, 1950); (e) ethyl acetate-pyridine-water (8:2:1) (White and Secor, 1953); (f) the same components in the proportion 10:4:3 (Whistler and Hickson, 1955); (g) 1-butanol-acetic acid-water (2:1:1) (Hough et al, 1950); (h) benzene-l-butanol-pyridine-water (1:5:3:3) . Methyl ethers of hexoses were separated in (i) 2-butanone-water (88.7:11.3) saturated with Borax (Dutton and Tanaka, 1962); (k) pyridine-ethyl acetate-glacial acetic acid-water (5:5:1:3) was used in chromatography of the uronic acids after the tank had been saturated with pyridine-ethyl acetate-water (11:40:6) (Fischer and Dorfel, 1955). The developed chromatograms were stained for carbohydrates with aniline oxalate (Horrocks, 1949) or silver nitrate according to Trevelyan et al (1950) andBenson et al (1952) or with triphenyltetrazolium chloride (Trevelyan et al, 1950; Wallenfels, 1950), which was employed as by Wallenfels, except that the spraying reagent was a freshly prepared mixture of 1 part of 0.5% triphenyltetrazolium chloride in absolute ethanol and 1 part of 1 n NaOH in 75% ethanol; /;-anisidine hydrochloride was used to detect uronic acids (Hough et al, 1949) and ninhydrin to check for amino acids (Toennies and Kolb, 1951).…”

Section: Methodsmentioning

confidence: 99%

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten^*

Springer¹,

Takahashi²,

Desai³

et al. 1965

Biochemistry

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Section: Methodsmentioning

confidence: 99%

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten^*

Springer¹,

Takahashi²,

Desai³

et al. 1965

Biochemistry

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“…Pyridine:ethyl acetate:acetic acid:water, (5:5:1:3 vlv, FISCHER and DORFEL, 1955). Saturation of the tank with the pyridine-ethyl acetatewater mixture was omitted.…”

Section: Paper Chromatographymentioning

confidence: 99%

Morphogenesis and Biochemical Processes in Schizophyllum Commune Fr.

Wessels

1965

Wentia

142

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The investigation concerns a first attempt to unravel biochemical patterns of significance for morphogenesis in S. commune. The results derive from two different stocks: one with wild morphology, the other exhibiting an impairment of pileus formation. Some morphological and genetical features of the stocks are discussed. A special culture technique allowed fair synchronization and control of development via the medium. This technique is based on the fact that differentiation can be prevented by cultivation in a thiamine‐free medium. Measured changes in protein, RNA, DNA, and soluble cell constituents during thiamine‐induced primordia formation yielded some information concerning the first steps of differentiation. Primordial growth was found to depend upon transport of nitrogenous compounds from the mycelium and the presence of a carbon source in the medium. Most of the assimilated glucose is deposited as polysaccharides in the cell walls of the primordia. Chemical analysis of the cell walls demonstrated that they are mainly composed of two different glucans, called here S‐ and R‐glucan, some properties of which were investigated. Pileus formation was shown to occur without the uptake of nutrients from the medium and to depend upon the withdrawal of substances from stunted fructifications and stroma in particular. Comparison of the two investigated stocks provided strong support for a causal relationship between degradation of the cell‐wall glucans and pileus formation. An analysis was made of respiration and fermentation during development; some findings probably bearing upon the problem of morphogenesis are discussed.

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“…50 filter paper, and developed with the solvent I (pyridine-ethyl acetate-acetic acid-water = 5:5:1:3, v/v) in a chamber saturated with the sovent II (pyridineethyl acetate-water = 11:40:6, v/v) for 49 hr (Fischer and Dörfel, 1955). The Spots were detected by TBA analysis (Warten, 1960), in which the paper strip was sprayed successively with 0.02 M NalCXj, with a mixed reagent (ethylen glycol-acetone-H 2 SO 4 = 50:50:0.3 v/v), and with 6 % TBA solution, then was heated at 100°C for 5 min.…”

Section: Paper Chromatographymentioning

confidence: 99%

Alginate Lyase Activities in the Extracts from Several Brown Algae.

Shiraiwa¹,

Abe²,

S³

et al. 1975

Botanica Marina

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Crudc enzymc extracts were prepared from several brown algae, and incubated with M C-alginatos, non-radioactivc alginates, short polymannuronide (SM) and -guluronide (SG), short mixed residue-polyuronide (SMG). The reuction products positive to TBA analysis werc fractionated by gcl-filtration. 1t was then found that unsaturated monomcr and dimer are the major products from SM whiJe mono-, di-and trimer consist mainly of the products from SG, suggosting the cxistcnce of two differcnt kinds of alginate lyases m the alga] extracts, The lyase activitics were hightr in old tissucs of the frond and lower in young ones, and in addition higher in st-asons when alga] fronds cmbedcd zoosporangia than in othcr seasons, Carbazolc analysis for reaction products showed thai absorbancc incrcased rcmurkably at carlicr stages of incubation while absorbance in TBA analysis incrcased almost in parallel with incubution time.(n a previous paper (Abe et aL, 1973), it was reported that mannuronic acid residue(M)-rich alginates are synthesized and metabolized in the frond more rapidly than guluronic acid residue(G)-rich alginates and MG-alginates, on the basis of results from 14 C-photoassimilation experiments. It seemed, therefore, to be of interest for us to elucidate these facts on an enzymic level.In regard to the enzymes which degrade alginates, at least two kinds of lyases have been known to exist in a mollusc, Dolabella auricula Solander and a few pseudomonads isolated from decayed fronds of brown algae and sea sand; poly-mannuronide (Nisizawa et #/., 1968) and ^guluronide (Kashiwabara et aL, 1969) lyases.On the other hand, it was recently reported that M-residues of the alginate chain are converted into G-residues without Splitting their glycosidic linkages by the action of an enzyme from Azotobacter vinelandi^ and äs a result alginates containing both uronic acid residues are formed (Haug and Larsen, 1972). It may then be postulated that there exist such kinds of epimerase in brown algae to play a part of the alginate metabolism. The present authors, therefore, attempted to obtain any enzyme preparation which participate in the alginate metabolism.Thus> they first prepared crude alginate lyase extracts from several brown algae and carried out some investigations at an enzymic level. This paper deals mainly with the results obtained for alginate lyases.Algae for experiments were collected at several coves in the vicinity of Shimoda Bay, and quickly transported to the laboratory to prepare crude enzyme solution. Algal species used were Spatoglosmm pacificum Yendo, Colpomenia sinuosa (Roth) Derbes et Solier, Endarachne binghamiae L Agarth,£/OT7M bicyclls (Kjellman) Setchell, Vndaria pinnatifida (Harvey) Suringes, Sargassum sagamianttrn Yendo, hhige okamurai Yendo, and hhige foliacea Okamura. Preparation of Crude Enzyme SolutionsFresh algal fronds (200 g) were made free from all macroscopic epiphytes by forceps in running sea water, and then shortly washed with tap water. They were mechanically ground on the folding surface of an ...

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Die papierchromatographische Trennung und Bestimmung der Uronsäuren

Cited by 132 publications

References 0 publications

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten^*

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten^*

Morphogenesis and Biochemical Processes in Schizophyllum Commune Fr.

Alginate Lyase Activities in the Extracts from Several Brown Algae.

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Die papierchromatographische Trennung und Bestimmung der Uronsäuren

Cited by 132 publications

References 0 publications

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten*

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten*

Morphogenesis and Biochemical Processes in Schizophyllum Commune Fr.

Alginate Lyase Activities in the Extracts from Several Brown Algae.

Contact Info

Product

Resources

About

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten^*

Cross Reactive Human Blood-Group H(O) Specific Polysaccharide from Sassafras albidum and Characterization of Its Hapten^*