Die Isolierung von Poliovirus in verschiedenen Gewebekulturen

Wigand, R.; Lang, F.; Adam, W.; Bauer, Heinz

doi:10.1007/bf02162308

Zeitschr. f. Hygiene.

1964

DOI: 10.1007/bf02162308

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Die Isolierung von Poliovirus in verschiedenen Gewebekulturen

R. Wigand

F. Lang

W. Adam

et al.

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1965

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Cited by 2 publications

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“…The significance level of the infectivity test would be increased if larger quantities could be examined. With respect to poliomyelitis and some other viruses pathogenic for man, the sizes of inoculum do not seem to be significantly different from those employed in techniques with FMDV (Van Hoosier et al, 1961;Howe, 1962;Kelly and Sanderson, 1962;Wigand et al, 1964). A noteworthy exception is a tissue culture method used in safety tests of poliomyelitis vaccines.…”

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Detection of Minimal Quantities of Foot-and-Mouth Disease Virus with Bovine Kidney Tissue Cultures

Gailiunas¹

1965

Applied Microbiology

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GAILIUNAS, PETER (Plum Island Animal Disease Laboratory, Greenport, N.Y.). Detection of minimal quantities of foot-and-mouth disease virus with bovine kidney tissue cultures. Appl. Microbiol. 13:872-875. 1965.-Minute quantities of foot-andmouth disease virus (FMDV) were suspended in 500-, 1,000-and 2,000-ml volumes, of virus growth medium and inoculated into primary bovine kidney cell cultures grown as monolayers in 5-liter Povitsky bottles. After 48 hr of incubation, the low concentrations of FMDV, containing from 10-3 to 10-'17 plaque-forming units (PFU) per milliliter of inoculum, multiplied to approximately 106 PFU per milliliter of fluid. The presence of extracellular virus after multiplication was verified by assaying the fluid in 4-oz prescription-bottle cultures. Minimal quantities of FMDV were able to infect the cells and replicate despite the large volume of the suspending fluid. This technique may be applicable for the detection of trace amounts of FMDV in clinical samples and vaccines. Skin samples of unknown infectivity in limited tests gave identical positive or negative results in both steers and Povitsky cultures, but were consistently negative when tested in suckling mice and in prescription-bottle tissue cultures.

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confidence: 99%

Detection of Minimal Quantities of Foot-and-Mouth Disease Virus with Bovine Kidney Tissue Cultures

Gailiunas¹

1965

Applied Microbiology

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Detection of Minimal Quantities of Foot-and-Mouth Disease Virus with Bovine Kidney Tissue Cultures

Gailiunas

1965

Appl Microbiol

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Minute quantities of foot-and-mouth disease virus (FMDV) were suspended in 500-, 1,000- and 2,000-ml volumes of virus growth medium and inoculated into primary bovine kidney cell cultures grown as monolayers in 5-liter Povitsky bottles. After 48 hr of incubation, the low concentrations of FMDV, containing from 10 -3 to 10 -1.7 plaque-forming units (PFU) per milliliter of inoculum, multiplied to approximately 10 6 PFU per milliliter of fluid. The presence of extracellular virus after multiplication was verified by assaying the fluid in 4- oz prescription-bottle cultures. Minimal quantities of FMDV were able to infect the cells and replicate despite the large volume of the suspending fluid. This technique may be applicable for the detection of trace amounts of FMDV in clinical samples and vaccines. Skin samples of unknown infectivity in limited tests gave identical positive or negative results in both steers and Povitsky cultures, but were consistently negative when tested in suckling mice and in prescription-bottle tissue cultures.

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Die Isolierung von Poliovirus in verschiedenen Gewebekulturen

Cited by 2 publications

References 7 publications

Detection of Minimal Quantities of Foot-and-Mouth Disease Virus with Bovine Kidney Tissue Cultures

Detection of Minimal Quantities of Foot-and-Mouth Disease Virus with Bovine Kidney Tissue Cultures

Detection of Minimal Quantities of Foot-and-Mouth Disease Virus with Bovine Kidney Tissue Cultures

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