Die Inkorporation von markiertem Sauerstoff aus Wasser in die ATP-, Kreatinphosphat- und Orthophosphat-Fraktion intakter Muskeln bei Ruhe, tetanischer Reizung und Erholung

Fleckenstein, A.; Gerlach, E.; Janke, J.; Marmier, P.

doi:10.1007/bf00362304

Cited by 39 publications

(6 citation statements)

References 20 publications

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“…This suggests that in resting fibres the exchange of the y phosphorus of the ATP is fairly rapid and similar to that of the arginine phosphate whereas the exchange of the a and , phosphorus atoms is comparatively slow. This is consistent both with work by Fleckenstein et al (1960) on the incorporation, via the intracellular orthophosphate, of 180 applied externally to frog muscle as H2180 into the a, fi and y phosphorus atoms of ATP and with work on the injection of 32P-labelled orthophosphate into squid giant axons (Caldwell et al 1964). Rough estimates of the probable specific activity of the y phosphorus atom of ATP in resting fibres can be made by multiplication of the over-all specific activity values for ATP given in Figs.…”

Section: Resultssupporting

confidence: 93%

“…This makes it very difficult to study the turnover of intracellular phosphorus compounds in intact cells if isotopically labelled compounds are applied extracellularly. In the case of frog muscle 180-labelled water has been used in an attempt to overcome this difficulty (Fleckenstein, Gerlach, Janke & Marmier, 1960;Janke, Marmier & Fleckenstein, 1965; Janke, Fleckenstein, Marmier & Koenig, 1966). The labelled water penetrates rapidly from the Ringer solution into the fibres where the 180 is incorporated first into the orthophosphate and subsequently into the ATP and creatine phosphate.…”

Section: Introductionmentioning

confidence: 99%

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The turnover of phosphorus compounds in crab muscle fibres.

Caldwell¹,

Walster²

1975

The Journal of Physiology

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SUMMARY1. The exchange of 32p injected into single crab muscle fibres as either orthophosphate or ATP has been studied. The results have been analysed in terms of a simple kinetic model.2. The exchange of 32p between ATP and arginine phosphate is considerably faster than that between ATP and orthophosphate during rest and during contraction in caffeine.3. The rate constants obtained at rest after the injection of either orthophosphate or ATP labelled with 32p were of the same order. They were also of the same order as those which have been obtained previously for squid axons.4. The exchange during caffeine contraction of 32p injected as orthophosphate gave rate constants which were consistent with an increased rate of splitting of ATP and with the rate constants needed to account for the observed net formation of orthophosphate. The results obtained after the injection of 32P-labelled ATP during caffeine contraction were less clear cut.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

The turnover of phosphorus compounds in crab muscle fibres.

Caldwell¹,

Walster²

1975

The Journal of Physiology

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“…If diffusion of PCr in vertebrate muscles is also the rate limiting step, our results are in contradiction with previous results in which the transference of phosphate from PCr to ADP was measured and in which the incorporation of the 32P into the intracellular high-energy phosphate compounds was attempted from the extracellular space, across the sarcolemma [Sacks, 1940;Sacks and Cleland, 1960;Fleckenstein and Janke, 1957;Fleckenstein, Gerlach Janke and Marmier, 1960]. If the rate of Lohman's ieaction in vertebrates is as large as in the case of the equivalent reaction in crustacea, then those previous experiments were carried out when the specific activities had reached equilibrium and, therefore, the phosphate exchange via the Lohman's reaction could not be detected.…”

Section: Discussioncontrasting

confidence: 99%

Phosphate Exchange Between High‐Energy Phosphate Compounds in Resting Crustacean Muscle

Alvarez¹,

Luxoro²,

Nassar-Gentina³

et al. 1980

Exp Physiol

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Single giant muscle fibres from Megabalanuiiis psittactis were microinjected with ATP-32P or internally perfused with a solution containing 32P-PArg in an attempt to demonstrate, in the absence of inhibitors, ATP utilization during mechanical activity with negative results. The rate constant of transfer of 32p from microinjected 32P-PArg to endogenous ATP was studied at two different temperatures, obtaining a Q1o of 108+0 12. It is concluded that the rate limiting step in the process of transference of phosphate in the resting muscle from balanus is the intracellular diffusion of PArg and not the rate at which the equivalent of Lohman's reaction in invertebrates proceeds.It is generally accepted that adenosine triphosphate (ATP) and phosphocreatine (PCr) are split during one of the steps of the contraction-recovery cycle in muscle from vertebrates [Wilkie, 1968a, b;Mommaerts, 1969;Mommaerts and Wallner, 1967;Woledge, 1971]. It is also thought that the ADP generated is rephosphorylated from PCr by the Lohman reaction so that no net change in concentration of ATP can be detected except when the Lohman reaction is blocked by 1-fluoro-2, 4-dinitrobenzene (FDNB;) [Cain and Davies, 1962]. In general ATP and PCr splitting reactions are thought to take place during the initial part of the mechanical events. In the measurement of the changes in ATP and PCr level in muscle one encounters some difficulties; chief among them are (1) arresting the chemical changes in the muscle by sufficiently rapid freezing and (2) making an extract of the frozen muscle containing all the substances of interest and analysing it without producing further chemical changes. Furthermore, the results are obtained by comparison of the substitute level in two muscles which, as a rule, are initially different. Although the method presented in the preceding paper resolves this last difficulty [Nassar-Gentina and Rojas, 1980], the rates of phosphate transfer under resting conditions have to be determined accurately before attempting to measure the effects of mechanical activation of the fibres.

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“…All recent chemical evidence is against the possibility that at the end of a short contraction in a normal muscle any net break-down of adenosin triphosphate (ATP) has occurred (Fleckenstein, Janke, Davies & Krebs, 1954;Mommaerts, 1954Mommaerts, , 1955Fleckenstein & Janke, 1957); while the experiments of Carlson & Siger (1960), with frog sartorii poisoned with 0 5 mM (1/11,000) IAA at 1-2°C, gave convincing evidence that no reduction of ATP had occurred when the muscles were frozen about 30 sec after the end of a short series of twitches and then analysed. Moreover, the experiments of Fleckenstein, Gerlach, Janke & Marmier (1960) suggested that 'the increased 018 incorporation, at least into ATP and creatine phosphate, was more correlated with metabolic reactions in recovery... than with the contraction process itself'. The possibility also that creatine phosphate continues to be split after a contraction is eliminated by such experiments as those of Nachmansohn (1928), ofMeyerhof & Nachmansohn (1930 and of Lehnartz (1931), who showed, on the contrary, that a considerable resynthesis occurs in 20 or 30 sec at room temperature.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the experiments of Fleckenstein, Gerlach, Janke & Marmier (1960) suggested that 'the increased 018 incorporation, at least into ATP and creatine phosphate, was more correlated with metabolic reactions in recovery... than with the contraction process itself'. The possibility also that creatine phosphate continues to be split after a contraction is eliminated by such experiments as those of Nachmansohn (1928), ofMeyerhof & Nachmansohn (1930 and of Lehnartz (1931), who showed, on the contrary, that a considerable resynthesis occurs in 20 or 30 sec at room temperature.…”

Section: Discussionmentioning

confidence: 99%

The negative delayed heat production in stimulated muscle

Hill¹,

Woledge²

1961

The Journal of Physiology

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In a paper on the delayed ('recovery') heat production of frog sartorius muscles, Hartree (1932 a) described experiments at O C in nitrogen which appeared to show an absorption of heat lasting for about 40 sec after tetanic contractions of 1-5 sec duration. This 'negative' heat production amounted, on the average, to 3 % of the initial heat, varying from 1 to 5 %.Six years earlier Furusawa & Hartree (1926), working with frog muscles in nitrogen at room temperature, had reported the frequent observation of early negative delayed heat after a short stimulus (0.05 sec or less); it amounted to 2-4 % of the initial heat. After a longer stimulus (0 3 sec) it was absent. They were inclined to regard it as an error due to irregular distribution of the heat production, though they had taken precautions to avoid such effects by using a special thermopile making contact with both faces of the muscle. In view of the results of the present paper it seems likely that the effect they observed was genuine: but their doubt about its reality is shown by the fact that they did not mention it in the summary of their paper. Two years later Hartree & Hill (1928, p. 210) reported 'in our experience there is never any negative heat'. Its possibility was in their minds, because Nachmansohn (1928) had just discovered that up to 30 % of the phosphagen split during a tetanus was resynthesized in about 20 sec at room temperature in the absence of oxygen: and he claimed that no lactic acid was formed at the same time, a result shown 3 years later, by the work of Lundsgaard (1931) and of Lehnartz (1931), to be wrong. Had his result about lactic acid been correct there might have been a large negative heat: though it is not evident then where the free energy could have come from to drive the resynthesis of the phosphagen.Because of the special interest of his result, and because of previous doubts and failures with negative heat, Hartree examined the conditions of his experiments very critically. The negative heat was small and could possibly have been due to several errors that might have affected his measurements. He was able to discard any possible errors which could be

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Die Inkorporation von markiertem Sauerstoff aus Wasser in die ATP-, Kreatinphosphat- und Orthophosphat-Fraktion intakter Muskeln bei Ruhe, tetanischer Reizung und Erholung

Cited by 39 publications

References 20 publications

The turnover of phosphorus compounds in crab muscle fibres.

The turnover of phosphorus compounds in crab muscle fibres.

Phosphate Exchange Between High‐Energy Phosphate Compounds in Resting Crustacean Muscle

The negative delayed heat production in stimulated muscle

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