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Schröer, Norbert

doi:10.1007/978-3-322-99357-1_6

Cited by 4 publications

(1 citation statement)

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“…The successful immobilization of a variety of cells in different matrices such as alginate, carrageenan, or polyacrylamide gels has been reported. [9][10][11] Immobilization of whole cells is generally more sensitive compared with isolated enzymes since cell viability is crucial to maintain high activity of the cells' intrinsic enzymes. Recently, the use of calcium alginate as an immobilization matrix for the recombinant E. coli whole cell biocatalyst for the enantioselective reduction of ethyl acetoacetate ͑EAA͒ to R-͑-͒ethyl hydroxybutyrate ͑EHB͒ has been described.…”

Section: Introductionmentioning

confidence: 99%

cBSA-147 for the preparation of bacterial biofilms in a microchannel reactor

Jaenicke

Eisele

et al. 2010

Biointerphases

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Whole cells are attractive biocatalysts, particularly if the reaction requires cofactors or involves multiple transformations. Immobilization of the catalyst is often a prerequisite for continuous processes. The highly cationic chemically modified plasma protein bovine serum albumin ͑cBSA-147͒ has been applied for the electrostatically mediated immobilization of the planktonic bacterium E. coli BL21 star (DE3), and the resulting biofilms were superior to those formed on poly-L-lysine coated surfaces. The biocatalyst was immobilized in a capillary column ͑inside diameter of 530 m and L =30 m͒ and evaluated in the enantioselective reduction of ethyl acetoacetate to R-͑-͒ethyl hydroxybutyrate. In continuous operation in the microreactor format, the productivity of the cells was about 30% higher than that determined in a bench-scale fermentation system. This increase is attributed to the improved mass transfer over short geometrical dimensions. The similarity in the results indicates that studies on a biofilm-coated microreactor can be used for the accelerated collection of data for process optimization.

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Section: Introductionmentioning

confidence: 99%