Die Elimination Rh-positiver fetaler Erythrozyten aus der Blutbahn rh-negativer Frauen durch körpereigene Isoagglutinine

Sieg, Wlodzimierz; Börner, P; Pixberg, H. U.; Deicher, H.

doi:10.1007/bf01632658

Cited by 5 publications

(2 citation statements)

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“…While many plasma proteins are also found in ISF, most of them such as albumin are found at much lower concentration due to capillary membrane and interstitial barriers [51][52] ISF is a rich source of biomarkers but it has been relatively unexplored due to lack of good sampling methods. Current methods like suction blister, microdialysis and open flow microperfusion cause significant skin trauma, are time consuming and require expert personnel and equipment to perform [8][9][10][11] . They also require sample processing steps to prepare samples for analysis.…”

Section: Binding Of R6g To Plasma Proteinsmentioning

confidence: 99%

“…ISF has been relatively unexplored largely due to limitations of sampling techniques such as suction blister 8 , which requires specialized equipment, takes ~1 h to perform and causes lasting skin wound; reverse iontophoresis 9 , which is limited to small molecules and requires frequent calibration; and microdialysis 10 and open flow microperfusion 11 , which requires expert personnel to perform minor surgery.…”

mentioning

confidence: 99%

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Plasmonic Paper Microneedle Patch for On-Patch Detection of Molecules in Dermal Interstitial Fluid

et al. 2019

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Minimally invasive devices to detect molecules in dermal interstitial fluid (ISF) are desirable for point-of-care diagnostic and monitoring applications. In this study, we developed a microneedle (MN) patch that collects ISF for on-patch biomarker analysis by surface-enhanced Raman scattering (SERS). The micron-scale MNs create micropores in the skin surface, through which microliter quantities of ISF are collected onto plasmonic paper on the patch backing. The plasmonic paper was prepared by immobilizing poly (styrene sulfonate) (PSS) coated gold nanorods (AuNRs) on a thin strip of filter paper using plasmonic calligraphy. Negatively charged PSS was used to bind positively charged rhodamine 6G (R6G), which served as a model compound, and thereby localize R6G on AuNR surface. R6G bound on the AuNR surface was detected and quantified by acquiring SERS spectra from the plasmonic paper MN patch. This approach was used to measure pharmacokinetic profiles of R6G in ISF and serum from rats in vivo. This proof-of-concept study indicates that a plasmonic paper MN patch has the potential to enable on-patch measurement of molecules in ISF for research and future medical applications.

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Section: Binding Of R6g To Plasma Proteinsmentioning

confidence: 99%

mentioning

confidence: 99%