Die Cytologie der Hepatocarcinogenese

Bannasch, Peter

doi:10.1007/978-3-642-80888-3_3

Cited by 11 publications

(11 citation statements)

References 217 publications

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“…The detailed histomorphology of AHF has been studied extensively by Bannasch and his co-workers (14,30). Their classification of AHF, which extends that of earlier proposals (26,29), is descriptive and includes clearcell, acidophilic-cell, intermediate-cell, tigroid-cell, basophilic-cell, and mixed-cell AHF (30).…”

Section: Morphologic Markersmentioning

confidence: 90%

“…Later, Bannasch and his associates (13,14) described the accumulation or glycogen or "glycogenosis" in focal lesions of livers of rats administered several hepatocarcinogens, including thioacetamide and nitrosomorpholine. Later, Bannasch and his associates (13,14) described the accumulation or glycogen or "glycogenosis" in focal lesions of livers of rats administered several hepatocarcinogens, including thioacetamide and nitrosomorpholine.…”

Section: And Later Kitagawa (12) Described Several Histochemical Altementioning

confidence: 99%

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Biochemical Markers Associated with the Stages of Promotion and Progression during Hepatocarcinogenesis in the Rat

Pitot¹,

Dragan²,

Sargent³

et al. 1991

Environmental Health Perspectives

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. The National Institute of Environmental Health Sciences (NIEHS) and Brogan & Partners are collaborating with JSTOR to digitize, preserve and extend access to Environmental Health Perspectives. Specific biochemical changes occurring during hepatocarcinogenesis have been sought by many investigators. The development of multistage models for hepatocarcinogenesis in the rodent has renewed interest in such marker alterations in preneoplastic as well as neoplastic hepatocytes. Preneoplastic altered hepatic foci (AHF) exhibit specific histomorphologic changes as viewed with tinctorial stains and show a variety of biochemical changes as evidenced by enzyme and immunohistochemistry and by other histochemical markers. During the reversible stage of promotion when AHF are scored by multiple markers, the distribution of markers within these lesions differs with the use of different promoting agents. One interpretation of this finding is that each promoting agent stimulates the replication of a set of initiated cells exhibitingthe phenotypic characteristics of a specific programmed phenotype. The same markers score AHF during the stage of progression, but many AHF in this stage are phenotypically heterogeneous, exhibiting in tissue sections a "focus-in-focus" pattern of marker alteration. These latter changes can be correlated with the appearance of karyotypic alterations in preneoplastic hepatocytes. On the other hand, it has been difficult to demonstrate the activation, either mutational or transcriptional, of proto-oncogenes until this stage of progression in rat hepatocarcinogenesis. Thus, a study of biochemical and molecular markers during the stages of hepatocarcinogenesis may lead to a better understanding of potential mechanisms involved in the development of neoplasia through the stages of initiation, promotion, and progression.

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Section: Morphologic Markersmentioning

confidence: 90%

Section: And Later Kitagawa (12) Described Several Histochemical Altementioning

confidence: 99%

Biochemical Markers Associated with the Stages of Promotion and Progression during Hepatocarcinogenesis in the Rat

Pitot¹,

Dragan²,

Sargent³

et al. 1991

Environmental Health Perspectives

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“…In our study, cystic liver was associated with only large polycystic kidneys. Experimentally, POlycystic liver and bile duct complexes, similar to those in man, have been produced by carcinogens (Bannasch 1975). In addition to liver cysts, renal cysts also become larger and more numerous with worsened serum creatinine in patients undergoing haemodialysis (Mickisch et al 1984).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the etiology of v. Meyenburg's complexes, from which the cysts in PLD originate, also associates with liver injury caused by alcohol or drugs in humans (Henning et al 1982, Karhunen et al 1985. Experimentally, POlycystic liver and bile duct complexes, similar to those in man, have been produced by carcinogens (Bannasch 1975).…”

Section: Discussionmentioning

confidence: 99%

Adult polycystic liver and kidney diseases are separate entities

Karhunen

Tenhu

1986

Clinical Genetics

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In 22 cases of either adult polycystic liver (PLD) or polycystic kidney (APCD) disease, considered as one dominantly inherited entity, both diseases occurred together only once. Early microscopical cystic lesions that are typical of PLD were found in another case of APCD. In this medicolegal autopsy series the incidence of PLD was 0.05% and that of APCD 0.08%.Cerebrdl haemorrhage or cerebral aneurysms wcrc found in 50% of APCD cases but in none of the cases with only PLD (~(0.01). Of the cases with PLD, 50% had associated renal cysts and 10% of the cases with APCD had associated liver cysts. The same medicolegal autopsy material yielded a prospective series of 95 male cases, where, however, kidney cysts were normally present in over 50% and liver cysts in about 20% of the cases of similar age. Thus, a part of the association between cystic disease of the liver and kidney may have been based on the common occurrence of cysts in old age.V. Meyenburg's complex was the microscopic alteration associated with cysts in PLD. It was associated as well with liver cysts in APCD suggesting that an intricate relation between PLD and APCD does occur in part of the cases, in this series characterized by large size APCD kidneys.The results indicate that in adults PLD is an entity of its own, expressed in most of the cases independently of APCD.

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“…Numerous papers concerning hepatoma cells have been published and will not be referred to here (for references see Bannasch. 1975).…”

Section: Detection Of Afp At the Ultrastructural Levelmentioning

confidence: 99%

Ultrastructural detection of alpha₁‐fetoprotein in hepatomas by use of peroxidase‐labelled antibodies

Kuhlmann

1978

Intl Journal of Cancer

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Hepatomas were induced i n rats by feeding Nnitrosomorpholine. Nodules from an alpha,-fetoprotein (AF P)-producing hepatoma were taken and subcutaneously injected into syngeneic hosts. From the f i r s t generation, the hepatoma producing most AFP was selected for further transfers. Four weeks after transplantation, the AFP concentration i n the host rats was in the range of 0. 8 to 1.5 mg AFP/ml serum. Transplanted hepatomas of the 14th to 19th generations were used for ultrastructural localization of AFP. Various methods of tissue-processing were examined under the light and electron microscopes for intracellular detection of AFP by use of specific anti-AFP antibodies conjugated with peroxidase. AFP was localized i n the perinuclear space, the rough-surfaced endoplasmic reticulum and the Golgi apparatus. Non-membranebound ribosomes did not stain for AFP. Fixation with 6% formaldehyde for 5 h followed by 6% formaldehyde plus 0.25-0.5% glutaraldehyde for 60-90 min proved best both for satisfactory ultrastructural conservation of the organs and for immunocytochemical localization of AFP. Concentrations of glutaraldehyde which exceeded 0.5% led t o a significant decrease i n immunocytochemical AFP reactions. O n the other hand, weak aldehyde fixation poorly preserved ultrastructural detail and leakage of insufficiently fixed material caused staining artifacts. Under our conditions, thick frozen sections from well-fixed hepatomas gave consistently reproducible results i n that intracellular penetration of antibody-peroxidase conjugates was superior t o hand-cut small tissue fragments. Tissue fixation and sampling were most important above all for other experimental steps.Synthesis of embryo-characteristic molecules by human and animal tumors is an oncologic phenomenon, and reexpression of fetal antigens has been reported for a number of tissues and tumor types (Abelev, 1968;Gold, 1971; Alexander, 1972 MATERIAL AND METHODS Induction and maintenance of hepatomasInbred male rats (strain BD X) were used throughout. Hepatomas were induced in 12-week-old animals by feeding the hepatotropic carcinogen N-nitrosomorpholine (Druckrey et al., 1967; Kuhlmann, 1978).Nodules from an AFP-producing hepatoma of the primary host rat were taken and subcutaneously injected into syngeneic 12-week-old hosts. When the inocula reached 1.5 cm in diameter, these were transferred: transplanted tumors were freed of necrotic areas, minced with razor blades into blocks of 2-3 mm and subcutaneously inoculated into hosts. From the first generation, the hepatoma producing most AFP (from rat No. 9 and designated as hepatoma H 9) was selected for further transfers. Immunological reagents and proceduresMonospecific rabbit anti-rat AFP immune sera were prepared (Kuhlmann, 1975). Pure rabbit anti-AFP antibodies were isolated from whole immune sera by use of immunoadsorbents, then IgG molecules were conjugated with horseradish peroxidase (HRP, RZ 3 ; Boehringer-Mannheim, Germany) and purified on a Sephadex G 200 column as described (Kuhlmann, 1975(Kuhlm...

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Die Cytologie der Hepatocarcinogenese

Cited by 11 publications

References 217 publications

Biochemical Markers Associated with the Stages of Promotion and Progression during Hepatocarcinogenesis in the Rat

Biochemical Markers Associated with the Stages of Promotion and Progression during Hepatocarcinogenesis in the Rat

Adult polycystic liver and kidney diseases are separate entities

Ultrastructural detection of alpha₁‐fetoprotein in hepatomas by use of peroxidase‐labelled antibodies

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Die Cytologie der Hepatocarcinogenese

Cited by 11 publications

References 217 publications

Biochemical Markers Associated with the Stages of Promotion and Progression during Hepatocarcinogenesis in the Rat

Biochemical Markers Associated with the Stages of Promotion and Progression during Hepatocarcinogenesis in the Rat

Adult polycystic liver and kidney diseases are separate entities

Ultrastructural detection of alpha1‐fetoprotein in hepatomas by use of peroxidase‐labelled antibodies

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Product

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Ultrastructural detection of alpha₁‐fetoprotein in hepatomas by use of peroxidase‐labelled antibodies