Die Biosynthese von Nucleins�uren und Proteinen in isolierten menschlichen Tonsillenzellen und ihre Abh�ngigkeit vom Lebensalter

Abstract

The aim of our investigations was to discriminate unspecific age-depending function of the tonsil. In an in-vitro system of tonsillar lymphocytes we could show, that there are differences in the DNA-, RNA- and Proteinbiosynthese between children and adults. The children organ is more active. It could be shown that the observed differences are only age depending and not influenced by the clinical state of the organ.

Description of Age-Depending Cellular Changes in the Human Tonsil

“…Takeuchi et al (1976) succeeded in identifying germinal centre cells to be B cells and Holowiecki et al (1975) found proliferating B cells in suspensions of tonsillar cells. The agedependence of thymidine incorporation was likewise proved (Siegel and Wilke, 1975a). From all these data it may be concluded that B cells proliferate in the germinal centres of the tonsil and that these processes decrease with advancing years.…”

“…An increase in cells is possible by proliferation and/or by accumulation of recirculating T cells. Proliferation of cells in the tonsil was found by numerous authors using different methods (Bömberg et al, 1968;Donovan and Soothill, 1973;Geha andMerler, 1974;Holowiecki et al, 1975;Mayer zum Gcttesberge and Koburg, 1967;Mitrou et al, 1969;Siegel and Wilke, 1975a, Sordat et al, 1969.Sprekelsen, 1975Staub et al, 1976;Stein, 1975;Suzuki and Ino, 1973). Therefore, proliferation processes may be considered to be one of the causes of the increase in cell quantity.…”

“…Age-dependent variations occur in the tissue concentrations of pyruvate and a-ketoglutarate, in the RNA and DNA concen trations as well as in the concentrations of IgG, IgA, IgM, and IgE (Wilke and Siegel, 1974; Wilke, 1975b, 1976). The incorporation of thymidine and uridine into the corresponding nucleic acids is reduced (Siegel and Wilke, 1975a). All these variations point to a non-specific decrease in the functional capacity of the organ.…”

“…Extraction of the tonsillar tissue according to the method used by Ogur and Rosen (1950) was already described (Siegel and Wilke, 1975a). The tonsils were frozen with liquid nitrogen immediately after surgery and used for analysing at once.…”