Die Belastung von Wässern mit gentoxischen Substanzen

Helma, Christoph; Knasmüller, Siegfried; Schulte‐Hermann, Rolf

doi:10.1007/bf02940361

Cited by 11 publications

(3 citation statements)

References 91 publications

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“…The most intensively applied assay for genotoxicity testing of water samples (>80% of all applications) ( , ) is the Salmonella/ microsome assay developed by Ames et al. ().…”

Section: Introductionmentioning

confidence: 99%

“…Although more than 50 genotoxicity assays can be used for water testing (14), the question of which short-term tests are most useful for the routine evaluation of water samples has not been fully adressed. Our aim was to study the perfomance of bacterial genotoxicity assays that are suited for the routine screening of large sample numbers.…”

Section: Introductionmentioning

confidence: 99%

“…The most intensively applied assay for genotoxicity testing of water samples (>80% of all applications) (14,15) is the Salmonella/microsome assay developed by Ames et al (11). This test is based on the detection of histidineindependent (his + ) revertants in selected Salmonella strains after exposure to mutagens.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Evaluation of Four Bacterial Assays for the Detection of Genotoxic Effects in the Dissolved Water Phases of Aqueous Matrices

Helma

Mersch-Sundermann

Houk

et al. 1996

Environ. Sci. Technol.

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The aim of this study was to evaluate the perfomance of four bacterial short-term genotoxicity assays (Salmonella/microsome assay, SOS Chromotest, Microscreen phage-induction assay, differential DNA repair test) that are widely used and/or have a promising potential for the genotoxicity testing of water samples. Twenty-three samples of different origins (drinking and bathing water, surface water, municipal and industrial wastewater, pulp mill effluents, groundwater, and landfill leachates) were tested in these assays. In total, 20 samples were genotoxic: 13 in the Salmonella/microsome assay, 13 in the SOS Chromotest, 8 in the Microscreen phage-induction assay, and 19 in the differential DNA repair test. Although the differential DNA repair test was the most sensitive system, positive results were obtained also with some of the negative control samples, and it had the least power to detect different genotoxic potencies. The Microscreen assay was the least sensitive system due to nonlinear results and sample toxicity. The Salmonella/microsome assay and the SOS Chromotest were of equal sensitivity, but the variance of the results was higher in the Salmonella/microsome assay. As the Salmonella/microsome assay also lacks toxicity correction for routine applications and ordinarily utilizes two strains, the SOS Chromotest appears to be the most promising test system for routine screening of water samples. Based on the present experiments, the investigated water samples were ranked according to their genotoxic potency as follows: landfill leachates > effluents from pulp production > wastewater > surface water > contaminated groundwater ≈ drinking and bathing water > control samples. The rankings obtained with the individual test systems were generally in good agreement. In addition, we present data on the impact of water treatment methods (activated sludge treatment, UV disinfection) and of alternative technologies (ozone vs ClO2 pulp bleaching) on the genotoxicity of water samples.

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“…The most intensively applied assay for genotoxicity testing of water samples (>80% of all applications) ( , ) is the Salmonella/ microsome assay developed by Ames et al. ().…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Evaluation of Four Bacterial Assays for the Detection of Genotoxic Effects in the Dissolved Water Phases of Aqueous Matrices

Helma

Mersch-Sundermann

Houk

et al. 1996

Environ. Sci. Technol.

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Unerwünschte Wirkungen im Gewässer: Gentoxizität als prioritärer Messparameter

Grummt

Hansen²,

Rettberg³

et al. 2004

Chemie in nserer Zeit

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Gentoxisch belastete aquatische Systeme sind eine potenzielle Gefahr für Mensch und Umwelt. Im Hinblick auf die weitreichenden Konsequenzen positiver Befunde für den regulatorischen Bereich, dessen Ziel es ist, Expositionen gegenüber gentoxischen Verbindungen zu verhindern oder soweit realisierbar gänzlich zu vermeiden, ist ein ausgereiftes experimentelles Konzept zwingend erforderlich. Die Biotests umu‐Test, Ames‐Test, Comet‐Assay, der DNA‐Aufwindungstest, die Alkalische Filterelution, der UDS‐Test und der Mikrokerntest sind ausreichend sensitiv, um gentoxische Effekte in komplexen Gemischen im Niedrig‐Dosis‐Bereich zu detektieren. Diese Tests lassen sich routinemäßig einsetzen und sollten daher Bestandteil der ersten Stufe einer Testbatterie zur Anzeige gentoxischen Gefährdungspotenzials in aquatischen Systemen sein.

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Tail Moment versus Tail Length—Application of an In Vitro Version of the Comet Assay in Biomonitoring for Genotoxicity in Native Surface Waters Using Primary Hepatocytes and Gill Cells from Zebrafish (Danio rerio)

Schnurstein

Braunbeck

2001

Ecotoxicology and Environmental Safety

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Die Belastung von Wässern mit gentoxischen Substanzen

Cited by 11 publications

References 91 publications

Comparative Evaluation of Four Bacterial Assays for the Detection of Genotoxic Effects in the Dissolved Water Phases of Aqueous Matrices

Comparative Evaluation of Four Bacterial Assays for the Detection of Genotoxic Effects in the Dissolved Water Phases of Aqueous Matrices

Unerwünschte Wirkungen im Gewässer: Gentoxizität als prioritärer Messparameter

Tail Moment versus Tail Length—Application of an In Vitro Version of the Comet Assay in Biomonitoring for Genotoxicity in Native Surface Waters Using Primary Hepatocytes and Gill Cells from Zebrafish (Danio rerio)

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