Die Bedeutung der �bermikroskopie f�r die Virusforschung

Rüska, H.; Borries, Bodo von; Руска, Э.

doi:10.1007/bf01243399

Cited by 75 publications

(23 citation statements)

References 11 publications

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“…This microscope had a much higher resolution than any of the light microscopes available at the time and promised to revolutionize many aspects of science, including cell biology and virology. Ernst Ruska was a physicist (1986 Nobel prize winner in physics), but his younger brother, Helmut Ruska, who had trained in medicine, rapidly recognized the potential of this microscope for investigating the nature of viruses . In the early 1940s, viruses were classified according to their hosts and the clinical symptoms they caused.…”

Section: Historymentioning

confidence: 99%

Virus detection by transmission electron microscopy: Still useful for diagnosis and a plus for biosafety

Roingeard

Raynal

Eymieux

et al. 2018

Reviews in Medical Virology

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Summary Transmission electron microscopy (TEM) is the only imaging technique allowing the direct visualization of viruses, due to its nanometer‐scale resolution. Between the 1960s and 1990s, TEM contributed to the discovery of many types of viruses and served as a diagnostic tool for identifying viruses directly in biological samples, either in suspension or in sections of tissues or mammalian cells grown in vitro in contact with clinical samples. The diagnosis of viral infections improved considerably during the 1990s, with the advent of highly sensitive techniques, such as enzyme‐linked immunosorbent assay (ELISA) and PCR, rendering TEM obsolete for this purpose. However, the last 20 years have demonstrated the utility of this technique in particular situations, due to its “catch‐all” nature, making diagnosis possible through visualization of the virus, without the need of prior assumptions about the infectious agent sought. Thus, in several major outbreaks in which molecular techniques failed to identify the infectious agent, TEM provided the answer. TEM is also still occasionally used in routine diagnosis to characterize infections not diagnosed by molecular assays. It is also used to check the microbiological safety of biological products. Many biopharmaceuticals are produced in animal cells that might contain little‐known, difficult‐to‐detect viruses. In this context, the “catch‐all” properties of TEM make it possible to document the presence of viruses or virus‐like particles in these products.

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Section: Historymentioning

confidence: 99%

Virus detection by transmission electron microscopy: Still useful for diagnosis and a plus for biosafety

Roingeard

Raynal

Eymieux

et al. 2018

Reviews in Medical Virology

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show abstract

“…The idea was based on Louis De Broglie's thesis of wave theory for particles, with the electron's short wavelength giving far higher resolution than was possible with light. Although the first EM pictures of eukaryotic cells are attributed to Keith Porter (Porter et al, 1945), it was Ernst Ruska's brother Helmut whose pioneering work in the field of infectology produced the first transmission electron microscopy (TEM) pictures of bacteria as well as viruses (Ruska et al, 1939). Imaging in these early days was limited to samples thin enough to allow the electrons through to the photographic plates located below; however, the technique was quickly adopted by biologists wanting to see beyond the limits of light microscopy.…”

Section: Where It All Beganmentioning

confidence: 99%

Is EM dead?

Knott

Genoud

2013

Journal of Cell Science

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SummarySince electron microscopy (EM) first appeared in the 1930s, it has held centre stage as the primary tool for the exploration of biological structure. Yet, with the recent developments of light microscopy techniques that overcome the limitations imposed by the diffraction boundary, the question arises as to whether the importance of EM in on the wane. This Commentary describes some of the pioneering studies that have shaped our understanding of cell structure. These include the development of cryo-EM techniques that have given researchers the ability to capture images of native structures and at the molecular level. It also describes how a number of recent developments significantly increase the ability of EM to visualise biological systems across a range of length scales, and in 3D, ensuring that EM will remain at the forefront of biology research for the foreseeable future.

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“…#18; #172; #172; Ruska et al 4. #19}, heralded a new era in analysing the complex geometric arrangement of biological cells and tissues at the nanometre scale.…”

mentioning

confidence: 99%

3-D EM exploration of the hepatic microarchitecture – lessons learned from large-volume in situ serial sectioning

Shami

Cheng

Huynh

et al. 2016

Sci Rep

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To-date serial block-face scanning electron microscopy (SBF-SEM) dominates as the premier technique for generating three-dimensional (3-D) data of resin-embedded biological samples at an unprecedented depth volume. Given the infancy of the technique, limited literature is currently available regarding the applicability of SBF-SEM for the ultrastructural investigation of tissues. Herein, we provide a comprehensive and rigorous appraisal of five different SBF-SEM sample preparation protocols for the large-volume exploration of the hepatic microarchitecture at an unparalleled X, Y and Z resolution. In so doing, we qualitatively and quantitatively validate the use of a comprehensive SBF-SEM sample preparation protocol, based on the application of heavy metal fixatives, stains and mordanting agents. Employing the best-tested SBF-SEM approach, enabled us to assess large-volume morphometric data on murine parenchymal cells, sinusoids and bile canaliculi. Finally, we integrated the validated SBF-SEM protocol with a correlative light and electron microscopy (CLEM) approach. The combination of confocal scanning laser microscopy and SBF-SEM provided a novel way to picture subcellular detail. We appreciate that this multidimensional approach will aid the subsequent research of liver tissue under relevant experimental and disease conditions.

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Die Bedeutung der �bermikroskopie f�r die Virusforschung

Cited by 75 publications

References 11 publications

Virus detection by transmission electron microscopy: Still useful for diagnosis and a plus for biosafety

Virus detection by transmission electron microscopy: Still useful for diagnosis and a plus for biosafety

Is EM dead?

3-D EM exploration of the hepatic microarchitecture – lessons learned from large-volume in situ serial sectioning

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